The Uni-Gold™ Streptococcus pneumoniae urinary antigen test: an interassay comparison with the BinaxNOW® Streptococcus pneumoniae test on consecutive urine samples and evaluation on patients with bacteremia

2015 ◽  
Vol 34 (8) ◽  
pp. 1583-1588 ◽  
Author(s):  
S. Athlin ◽  
O. Altun ◽  
H. B. Eriksen ◽  
V. Özenci ◽  
K. Strålin
Keyword(s):  
Streptococcus Pneumoniae ◽  
Urine Samples ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test

Detection of Streptococcus pneumoniae in Urine by Loop-Mediated Isothermal Amplification

2020 ◽  
Author(s):  
María Dolores Cima-Cabal ◽  
Emma Vázquez-Espinosa ◽  
Fernando Vazquez ◽  
María del Mar García-Suárez
Keyword(s):  
Streptococcus Pneumoniae ◽  
Predictive Value ◽  
Pneumococcal Pneumonia ◽  
Lamp Assay ◽  
Urine Samples ◽  
Healthy Children ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Free Dna

Abstract Objective To assess the loop-mediated isothermal amplification (LAMP) to detect cell-free DNA from Streptococcus pneumoniae in urine samples from children with pneumococcal pneumonia. Methods LAMP reactions using four primers (backward inner primer, forward inner primer, B3, and F3) targeting conserved regions of the S. pneumoniae ply gene and DNA from the recombinant plasmid pTrc99A-ply were optimized for temperature (65°C) and MgSO4 concentration (8 mM) conditions. Urine samples from 71 patients with symptoms of pneumonia and from 17 healthy children were tested side by side using the isothermal methodology LAMP and the commercial urinary antigen test, BinaxNOW S. pneumoniae assay. Percentages of sensitivity, specificity, positive predictive value (PPV), negative predictive value, and positive (LR) were calculated to compare both tests. Results The specificity of the LAMP reaction was confirmed against several species of bacteria and yeast that can cause pneumonia or urine infections. The suitability of the LAMP assay was evaluated in urine samples from 71 patients and 17 healthy children. All patients (100%) with confirmed pneumococcal pneumonia were positive for the LAMP assay. Among patients with possible/probable pneumonia, 74.1% were identified as positive using the LAMP test. Notably, a higher specificity (95.4%), PPV (94.1%) and positive LR (21.7) were found compared with the urinary antigen test. Conclusion The presence of S. pneumoniae cell-free DNA in urine samples of pediatric patients can be used as a specific diagnostic biomarker for community-acquired pneumonia by using the LAMP methodology.

Usefulness of Streptococcus pneumoniae urinary antigen test recommended in the management of community-acquired pneumonia in cancer patients.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e20679-e20679
Author(s):  
Paul Louis Woerther ◽  
Sami Antoun ◽  
Arif Alibay ◽  
Sylvie Khan ◽  
Bertrand Gachot ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Cancer Patients ◽  
Community Acquired Pneumonia ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test

scholarly journals Method Comparison of the ImmuView L. pneumophila and L. longbeachae Urinary Antigen Test with the BinaxNOW Legionella Urinary Antigen Card for Detection of Legionella pneumophila Serogroup 1 Antigen in Urine

2019 ◽  
Vol 58 (3) ◽  
Author(s):  
Paul Badoux ◽  
Lianne Kracht-Kosten ◽  
Bjorn Herpers ◽  
Sjoerd Euser
Keyword(s):  
Sensitivity And Specificity ◽  
Legionella Pneumophila ◽  
Limit Of Detection ◽  
Method Comparison ◽  
Urine Samples ◽  
Analytical Sensitivity ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Content Type

ABSTRACT We compared the clinical performance of the ImmuView L. pneumophila and L. longbeachae urinary antigen test (SSI Diagnostica A/S, Hillerød, Denmark) to that of the BinaxNOW Legionella urinary antigen card (Binax; Abbott, Lake Buff, IL) using urine specimens from patients suspected of having pneumonia. In total, 100 frozen urine samples (derived from 50 Legionella cases and 50 noncases) were analyzed with both tests, as were 200 nonfrozen prospectively collected samples. For urine samples from five Legionella cases and two non-Legionella cases, analytical sensitivity (limit of detection) and repeatability were examined. The urine samples from the five Legionella cases were diluted with urine samples that tested Legionella urinary antigen negative with both tests. The analyses of the 100 frozen samples resulted in a sensitivity and specificity of both ImmuView and the BinaxNOW of 96.0% (48/50) and 100% (50/50), respectively. Of the 200 nonfrozen samples, there were three samples that showed a positive result for L. pneumophila by both tests. The analyses of reproducibility showed that for the 34 (diluted) samples that were tested at two consecutive times, 33 samples showed a consistent result for both the ImmuView and the BinaxNOW tests (Cohen’s kappa values of 0.916 and 0.928). In addition, the ImmuView test may have detected two L. longbeachae-positive urine samples, although other diagnostic tests could not confirm this. Both ImmuView and BinaxNOW showed high sensitivity and specificity for the detection of L. pneumophila serogroup 1 antigen in urine samples from clinical patients with a suspected lower respiratory tract infection.

scholarly journals Evaluation of the bioNexia Legionella Test, Including Impact of Incubation Time Extension, for Detection of Legionella pneumophila Serogroup 1 Antigen in Urine

2017 ◽  
Vol 55 (6) ◽  
pp. 1733-1737 ◽  
Author(s):  
Paul Badoux ◽  
Sjoerd M. Euser ◽  
Jacob P. Bruin ◽  
Patrick P. G. Mulder ◽  
Ed P. F. Yzerman
Keyword(s):  
Legionella Pneumophila ◽  
Incubation Time ◽  
Urine Samples ◽  
Lower Sensitivity ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Prolonged Incubation ◽  
Content Type ◽  
Time Extension

ABSTRACTIn this study, we compared the bioNexia test (bioMérieux, Marcy-l'Étoile, France), a new immunochromatographic assay for the detection ofLegionella pneumophilaserogroup 1 in urine, with the BinaxNOW urinary antigen test (Alere, Waltham, Massachusetts, USA). After 15 min of incubation (in accordance with the manufacturers' instructions), the sensitivities and specificities were, respectively, 76.5% and 97.2% for the bioNexia test and 87.1% and 100% for the BinaxNOW test. After a prolonged incubation time of 60 min, the sensitivities and specificities increased to, respectively, 89.4% and 97.2% for the bioNexia test and 91.8% and 100% for the BinaxNOW test. When the tests were read after 15 min, the concentration of discrepant urine samples increased the sensitivities to 94.1% for both tests. In conclusion, we found that although the bioNexia test showed lower sensitivity for the detection ofL. pneumophilaantigen in nonconcentrated urine compared to the BinaxNOW test, a prolonged incubation time as well as the use of concentrated samples showed comparable sensitivities for both tests.

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