scholarly journals Method Comparison of the ImmuView L. pneumophila and L. longbeachae Urinary Antigen Test with the BinaxNOW Legionella Urinary Antigen Card for Detection of Legionella pneumophila Serogroup 1 Antigen in Urine

2019 ◽  
Vol 58 (3) ◽  
Author(s):  
Paul Badoux ◽  
Lianne Kracht-Kosten ◽  
Bjorn Herpers ◽  
Sjoerd Euser
Keyword(s):  
Sensitivity And Specificity ◽  
Legionella Pneumophila ◽  
Limit Of Detection ◽  
Method Comparison ◽  
Urine Samples ◽  
Analytical Sensitivity ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Content Type

ABSTRACT We compared the clinical performance of the ImmuView L. pneumophila and L. longbeachae urinary antigen test (SSI Diagnostica A/S, Hillerød, Denmark) to that of the BinaxNOW Legionella urinary antigen card (Binax; Abbott, Lake Buff, IL) using urine specimens from patients suspected of having pneumonia. In total, 100 frozen urine samples (derived from 50 Legionella cases and 50 noncases) were analyzed with both tests, as were 200 nonfrozen prospectively collected samples. For urine samples from five Legionella cases and two non-Legionella cases, analytical sensitivity (limit of detection) and repeatability were examined. The urine samples from the five Legionella cases were diluted with urine samples that tested Legionella urinary antigen negative with both tests. The analyses of the 100 frozen samples resulted in a sensitivity and specificity of both ImmuView and the BinaxNOW of 96.0% (48/50) and 100% (50/50), respectively. Of the 200 nonfrozen samples, there were three samples that showed a positive result for L. pneumophila by both tests. The analyses of reproducibility showed that for the 34 (diluted) samples that were tested at two consecutive times, 33 samples showed a consistent result for both the ImmuView and the BinaxNOW tests (Cohen’s kappa values of 0.916 and 0.928). In addition, the ImmuView test may have detected two L. longbeachae-positive urine samples, although other diagnostic tests could not confirm this. Both ImmuView and BinaxNOW showed high sensitivity and specificity for the detection of L. pneumophila serogroup 1 antigen in urine samples from clinical patients with a suspected lower respiratory tract infection.

scholarly journals Evaluation of the bioNexia Legionella Test, Including Impact of Incubation Time Extension, for Detection of Legionella pneumophila Serogroup 1 Antigen in Urine

2017 ◽  
Vol 55 (6) ◽  
pp. 1733-1737 ◽  
Author(s):  
Paul Badoux ◽  
Sjoerd M. Euser ◽  
Jacob P. Bruin ◽  
Patrick P. G. Mulder ◽  
Ed P. F. Yzerman
Keyword(s):  
Legionella Pneumophila ◽  
Incubation Time ◽  
Urine Samples ◽  
Lower Sensitivity ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Prolonged Incubation ◽  
Content Type ◽  
Time Extension

ABSTRACTIn this study, we compared the bioNexia test (bioMérieux, Marcy-l'Étoile, France), a new immunochromatographic assay for the detection ofLegionella pneumophilaserogroup 1 in urine, with the BinaxNOW urinary antigen test (Alere, Waltham, Massachusetts, USA). After 15 min of incubation (in accordance with the manufacturers' instructions), the sensitivities and specificities were, respectively, 76.5% and 97.2% for the bioNexia test and 87.1% and 100% for the BinaxNOW test. After a prolonged incubation time of 60 min, the sensitivities and specificities increased to, respectively, 89.4% and 97.2% for the bioNexia test and 91.8% and 100% for the BinaxNOW test. When the tests were read after 15 min, the concentration of discrepant urine samples increased the sensitivities to 94.1% for both tests. In conclusion, we found that although the bioNexia test showed lower sensitivity for the detection ofL. pneumophilaantigen in nonconcentrated urine compared to the BinaxNOW test, a prolonged incubation time as well as the use of concentrated samples showed comparable sensitivities for both tests.

scholarly journals Urinary antigene and PCR can both be used to detect Legionella pneumophila in children’s hospital-acquired pneumonia

2019 ◽  
Vol 29 (2) ◽  
Author(s):  
Sayed-Yousef Mojtahedi ◽  
Aliakbar Rahbarimanesh ◽  
Samileh Noorbakhsh ◽  
Hossein Shokri ◽  
Saeedreza Jamali-Moghadam-Siyahkali ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Pediatric Intensive Care ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Legionella Pneumonia ◽  
Hospital Acquired Pneumonia ◽  
The Difference ◽  
Underlying Diseases ◽  
Hospital Acquired

Legionella pneumophila is the causative agent of more than 95% cases of severe Legionella pneumonia. Nosocomial pneumonias in different hospital wards is an important medical and pharmaceutical concern. This study aimed to detect Legionella with two methods: polymerase chain reaction (PCR) and detection of urine antigenic test (UAT) in patients suffering from nosocomial pneumonia admitted to pediatric intensive care unit (PICU) of children hospitals. This study was conducted in PICU wards of Rasool Akram and Bahrami children hospitals, Tehran, Iran during 2013 - 2014. In patients diagnosed with hospital-acquired pneumonia, intratracheal secretion samples for PCR and urine sample for UAT were taken. Simultaneously, PCR and urinary antigen test were conducted using commercial kits. The results of urinary antigen test and PCR were analyzed by SPSS v.19 for statistical comparison. In this study, 96 patients aging 2.77 years on average with two age peaks of less than 1 year and 7-8 year were enrolled. More than half of the patients were under 1 year old. The most common underlying diseases were seizure, Acute Lymphoblastic Lymphoma, Down syndrome and metabolic syndromes. The positivity rate of Legionella urinary antigen test was 16.7% and positivity rate of PCR test was 19.8%. There were no significant associations between the results obtained by both assays with age, gender or underlying diseases. In conclusion, PCR is a better detection method for Legionella infection than urinary antigen test, but the difference between the two methods was not significant.

scholarly journals Unusual clinical picture in an overweight patient with Legionella pneumophila: A case report

2017 ◽  
Vol 4 (3) ◽  
pp. 31
Author(s):  
Leonidas Grigorakos ◽  
Daria Lazarescu ◽  
Anthi Georgiadou ◽  
Maria Bikou ◽  
Magda Gkouni ◽  
...  
Keyword(s):  
Case Report ◽  
Legionella Pneumophila ◽  
Clinical Picture ◽  
Clinical Condition ◽  
Antigen Test ◽  
Overweight Patient ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Legionnaires Disease ◽  
Continue Therapy

This case report describes a case of a patient with Legionnaires’ disease (LD) manifested three days upon his return from a medical conference, which took place in a hotel close to the seaside. Our patient presented to the hospital febrile, weak, confused and with mild difficulty in breathing. After being subjected to several tests, he was diagnosed with LD. Even though his initial urinary antigen test (UAT) was negative, subsequent immunofluorescent assays (IFA) were positive for Legionella pneumophila (LP). The patient was immediately initiated specific antibiotics therapy and supportive measures. After 11 days he was released from the hospital with considerable melioration of his clinical condition and with specific instructions to continue therapy at home.

scholarly journals Comparison of the Novel Immunocatch Legionella Test with Sofia Legionella FIA Assay and with BinaxNOW Legionella Card Assay for Detection ofLegionella pneumophila(Serogroup 1) Antigen in Urine Samples

2019 ◽  
Vol 57 (8) ◽  
Author(s):  
F. Congestrì ◽  
M. Morotti ◽  
R. Vicari ◽  
M. F. Pedna ◽  
M. Sparacino ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Signs And Symptoms ◽  
Laboratory Diagnosis ◽  
Antigen Detection ◽  
Urine Samples ◽  
Urinary Antigen ◽  
Content Type ◽  
High Clinical Suspicion ◽  
Legionnaires Disease ◽  
Urinary Antigen Detection

ABSTRACTLegionnaires’ disease (LD) refers to a serious form of acute pneumonia caused byLegionellaspecies. LD can be difficult to diagnose because the signs and symptoms are nonspecific, and therefore a rapid laboratory diagnosis is of paramount importance. In this study, a recently introduced immunochromatographic test (Immunocatch Legionella; Eiken Chemical Co., Ltd.) forLegionella pneumophila(serogroup 1) urinary antigen detection was compared with the Sofia Legionella fluorescent immunoassay (FIA) (Quidel) (routinely used in our laboratory) and with the widely used BinaxNOW Legionella assay (Alere). A total of 248 urine samples (60 frozen and 188 fresh) were evaluated. All of the samples were collected from patients with high clinical suspicion of Legionnaires’ disease. The three assays were performed simultaneously according to the manufacturers’ instructions. A total of 180 concordant negative and 66 concordant positive results were obtained. Only 2 discrepant results were registered. The sensitivity and specificity of Immunocatch compared with Sofia were, respectively, 98.5% and 99.4%. Cohen's kappa coefficient and overall percent agreement between Immunocatch and Sofia were also calculated and resulted in, respectively, 0.97 and 99.2%. These performances suggest that the Immunocatch test is a useful tool forLegionella pneumophila(serogroup 1) urinary antigen detection.

Clinical utility of urinary antigen test and molecular method for detection of Legionella pneumophila.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Thana’a R AbdulRahman
Keyword(s):  
Legionella Pneumophila ◽  
Point Of View ◽  
P Value ◽  
Antigen Test ◽  
Rt Pcr ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Serum Samples ◽  
High Prevalence ◽  
Legionnaires Disease

Legionella pneumophila is gram-negative bacterium which causes Legionnaires’ disease and Pontiac fever.To determine the frequency of serogroup 1 and other serogroup of Legionellapneumophila in pneumonic patients and the clinical utilityofLegionellapneumoniaurinary antigen test (LPUAT)in terms of sensitivity and specificity andcomparethe results with q Real Time PCR using serum samples. A total of 100 pneumonic patients were enrolled in this studyduring a period between October 2016 to April 2017; all patients under therapy with antibiotics.Serum and urine specimens were obtained from all patients; urine samples were processed for urinary antigen test. Serum samples were collected and submitted to DNA extraction for detection of L. pneumophila mip gene by q RT PCRassay. The percentage of L. pneumophila in two hospitals In Baghdad was 30%. Of these 26% was serogroup 1 detected by UAT. In the other hand, 23 % of samples were positive by q RT- PCR based mip gene,of these 19 % were serogroup 1 and 4% were other serogroups. The sensitivity of UAT is high (P value< 0.001), which means statistically highlysignificance than q RT PCR. Legionellapneumophila urinary antigen test is a rapid tool for early diagnosis of Legionella infection which highlights the need of using this test in hospitals and health institutions and there is a high prevalence of L.pneumophila in Iraqthat refer to the necessity of considering this microorganism point of view in future studies for detection and treatment in pneumonic patients.

Detection of Streptococcus pneumoniae in Urine by Loop-Mediated Isothermal Amplification

2020 ◽  
Author(s):  
María Dolores Cima-Cabal ◽  
Emma Vázquez-Espinosa ◽  
Fernando Vazquez ◽  
María del Mar García-Suárez
Keyword(s):  
Streptococcus Pneumoniae ◽  
Predictive Value ◽  
Pneumococcal Pneumonia ◽  
Lamp Assay ◽  
Urine Samples ◽  
Healthy Children ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Free Dna

Abstract Objective To assess the loop-mediated isothermal amplification (LAMP) to detect cell-free DNA from Streptococcus pneumoniae in urine samples from children with pneumococcal pneumonia. Methods LAMP reactions using four primers (backward inner primer, forward inner primer, B3, and F3) targeting conserved regions of the S. pneumoniae ply gene and DNA from the recombinant plasmid pTrc99A-ply were optimized for temperature (65°C) and MgSO4 concentration (8 mM) conditions. Urine samples from 71 patients with symptoms of pneumonia and from 17 healthy children were tested side by side using the isothermal methodology LAMP and the commercial urinary antigen test, BinaxNOW S. pneumoniae assay. Percentages of sensitivity, specificity, positive predictive value (PPV), negative predictive value, and positive (LR) were calculated to compare both tests. Results The specificity of the LAMP reaction was confirmed against several species of bacteria and yeast that can cause pneumonia or urine infections. The suitability of the LAMP assay was evaluated in urine samples from 71 patients and 17 healthy children. All patients (100%) with confirmed pneumococcal pneumonia were positive for the LAMP assay. Among patients with possible/probable pneumonia, 74.1% were identified as positive using the LAMP test. Notably, a higher specificity (95.4%), PPV (94.1%) and positive LR (21.7) were found compared with the urinary antigen test. Conclusion The presence of S. pneumoniae cell-free DNA in urine samples of pediatric patients can be used as a specific diagnostic biomarker for community-acquired pneumonia by using the LAMP methodology.

scholarly journals The impact of diagnosis by legionella urinary antigen test on the epidemiology and outcomes of Legionnaires' disease

2001 ◽  
Vol 127 (2) ◽  
pp. 275-280 ◽  
Author(s):  
N. FORMICA ◽  
M. YATES ◽  
M. BEERS ◽  
J. CARNIE ◽  
G. HOGG ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Community Acquired Pneumonia ◽  
Antigen Test ◽  
Urinary Antigen ◽  
Urinary Antigen Test ◽  
Disease Epidemiology ◽  
The Public ◽  
Life Threatening ◽  
Legionnaires Disease ◽  
The Impact

Legionnaires' disease is an uncommon but important cause of life-threatening community-acquired or nosocomial pneumonia. The urinary antigen enzyme immunoassay test, used in Victoria since 1995, now accounts for the majority of initial laboratory notifications (81% in 1999). We review the impact of the test on the disease epidemiology and the public health investigative process. We focus on the major subgroup of cases due to Legionella pneumophila serogroup 1, comparing delays until notification and mortality for urinary antigen detected cases with culture detected cases. The urinary antigen test facilitates a 5-day reduction for the delay between onset of illness and notification. We observed that there was minimal clinical heterogeneity of urinary antigen detected cases whether they were subsequently culture confirmed or not. We encourage clinician use of the urinary antigen test in cases of community-acquired pneumonia where Legionnaires' disease is a possible diagnosis, in conjunction with culture of clinical specimens.

scholarly journals Comparative Evaluation of the Novel bioNexia Legionella Test with the BinaxNOW Legionella Card Assay and the Sofia Legionella FIA Assay for Detection of Legionella pneumophila (Serogroup 1) Antigen in Urine Samples

2016 ◽  
Vol 54 (4) ◽  
pp. 1164-1166 ◽  
Author(s):  
Francesco Congestrì ◽  
Elisabetta Crepaldi ◽  
Marina Gagliardi ◽  
Maria Federica Pedna ◽  
Vittorio Sambri
Keyword(s):  
Legionella Pneumophila ◽  
Comparative Evaluation ◽  
Urine Samples ◽  
The Novel ◽  
Immunochromatographic Test ◽  
Urinary Antigen ◽  
Content Type

A new immunochromatographic test (bioNexiaLegionella; bioMérieux) for the detection ofLegionella pneumophilaurinary antigen was evaluated in 255 urine samples. The results were compared with those obtained by the BinaxNOW and SofiaLegionellatests. The novel test compared well with those currently in use.

Sign in / Sign up

Export Citation Format

Share Document