Evaluation of the PREVI® Isola automated seeder system compared to reference manual inoculation for antibiotic susceptibility testing by the disk diffusion method

2015 ◽  
Vol 34 (9) ◽  
pp. 1859-1869 ◽  
Author(s):  
S. Le Page ◽  
A. van Belkum ◽  
C. Fulchiron ◽  
R. Huguet ◽  
D. Raoult ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Antibiotic Susceptibility Testing ◽  
Disk Diffusion Method ◽  
Reference Manual

scholarly journals Standardization of Operator-Dependent Variables Affecting Precision and Accuracy of the Disk Diffusion Method for Antibiotic Susceptibility Testing

2015 ◽  
Vol 53 (12) ◽  
pp. 3864-3869 ◽  
Author(s):  
Michael Hombach ◽  
Florian P. Maurer ◽  
Tamara Pfiffner ◽  
Erik C. Böttger ◽  
Reinhard Furrer
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Antibiotic Susceptibility Testing ◽  
Content Type ◽  
E Coli ◽  
The Mean ◽  
Precision And Accuracy ◽  
Standard Deviations

Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not been systematically investigated yet. This study analyzed whether photometrically controlled inoculum preparation and/or automated inoculation could further improve the standardization of disk diffusion. Suspensions ofEscherichia coliATCC 25922 andStaphylococcus aureusATCC 29213 of 0.5 McFarland standard were prepared by 10 operators using both visual comparison to turbidity standards and a Densichek photometer (bioMérieux), and the resulting CFU counts were determined. Furthermore, eight experienced operators each inoculated 10 Mueller-Hinton agar plates using a single 0.5 McFarland standard bacterial suspension ofE. coliATCC 25922 using regular cotton swabs, dry flocked swabs (Copan, Brescia, Italy), or an automated streaking device (BD-Kiestra, Drachten, Netherlands). The mean CFU counts obtained from 0.5 McFarland standardE. coliATCC 25922 suspensions were significantly different for suspensions prepared by eye and by Densichek (P< 0.001). Preparation by eye resulted in counts that were closer to the CLSI/EUCAST target of 108CFU/ml than those resulting from Densichek preparation. No significant differences in the standard deviations of the CFU counts were observed. The interoperator differences in standard deviations when dry flocked swabs were used decreased significantly compared to the differences when regular cotton swabs were used, whereas the mean of the standard deviations of all operators together was not significantly altered. In contrast, automated streaking significantly reduced both interoperator differences, i.e., the individual standard deviations, compared to the standard deviations for the manual method, and the mean of the standard deviations of all operators together, i.e., total methodological variation.

scholarly journals Inaccuracy of the Disk Diffusion Method Compared with the Agar Dilution Method for Susceptibility Testing of Campylobacter spp.

2011 ◽  
Vol 50 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Mirva Lehtopolku ◽  
Pirkko Kotilainen ◽  
Pauli Puukka ◽  
Ulla-Maija Nakari ◽  
Anja Siitonen ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Disk Diffusion Method ◽  
Agar Dilution ◽  
Campylobacter Spp

Clarithromycin and Amoxicillin Susceptibility Testing ofHelicobacter pyloriby Disk Diffusion Method

2009 ◽  
Vol 12 (1) ◽  
pp. 30 ◽  
Author(s):  
Heungsup Sung ◽  
Jung-Oak Kang ◽  
Mi Ae Lee ◽  
Jongwook Lee ◽  
Hae Kyung Lee ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method
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