scholarly journals Diagnostic methods for Helicobacter pylori infection: ideals, options, and limitations

2018 ◽  
Vol 38 (1) ◽  
pp. 55-66 ◽  
Author(s):  
Parisa Sabbagh ◽  
Mousa Mohammadnia-Afrouzi ◽  
Mostafa Javanian ◽  
Arefeh Babazadeh ◽  
Veerendra Koppolu ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection

Evaluation Study of Three Diagnostic Methods for Helicobacter pylori Infection

2017 ◽  
pp. 128-138
Author(s):  
Kameran Ali ◽  
◽  
Hassan tawfeeq ◽  
Ayad Ali
Keyword(s):  
Helicobacter Pylori ◽  
Evaluation Study ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection

scholarly journals The role and diagnostic value of the most common methods for diagnosing Helicobacter pylori infection

2020 ◽  
Vol 11 (4) ◽  
pp. 59-66
Author(s):  
Alexander V. Tryapitsyn ◽  
Vladimir A. Mal’kov
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
False Negative ◽  
High Sensitivity ◽  
Diagnostic Value ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Unexpected Result ◽  
Optimal Method ◽  
Histochemical Test

The purpose of the study was to identify and evaluate the practical value of the most common diagnostic methods for Helicobacter pylori infection in patients with chronic inflammation of the gastric mucosa. Materials and methods. The study involved 104 people. All the patients examined underwent esophagogastroduodenoscopy with a rapid urease histochemical test for Helicobacter pylori and a standard five-point biopsy of the gastric mucosa for morphological evaluation and bacterioscopy. If the result of the quick urease histochemical test coincided with the data of the biopsy study on Helicobacter pylori, no further examination was carried out. In case of discrepancy the patients additionally underwent the 13C-urease breath test. Results. It was found that the sensitivity of the rapid urease histochemical test in the area of the proposed model, according to our study, was 89.74% and the specificity was 46.15% when validating it using the additional methods. Similar calculations for the diagnostic method of staining by Giemsa showed sensitivity of 100% and specificity of 97.4%. The total number of the infected in the survey was 78 out of 104 people, which amounted to 75%. When analysing the severity of activity, inflammation and atrophic changes in the gastric mucosa, it was found that in the patients with two positive tests, the severity of the processes was significantly higher than in those with one positive test or all the tests were negative (p 0.05). When comparing the same processes in cases where only a quick urease histochemical test was positive and no signs of Helicobacteriosis were detected in any test, no significant differences were detected. Discussion. The rapid urease histochemical test showed good sensitivity of 89.74%, but unsatisfactory specificity of 46.15%, which severely limits its use. An unexpected result for this methodology was a large number of false positive tests, while the European recommendations indicate a more frequent occurrence of false negative results, which can be explained by differences in the production technology of the test systems. High sensitivity and specificity of the Helicobacter pylori test using Giemsa staining strongly depends on observing the methodology and experience of a specialist and cannot be recommended as a standard in the routine medical use. The study confirmed the recommendations for using at least two diagnostic tests to diagnose the infection, while the most common rapid urease histochemical test always needs confirmation. As a result of the analysis, it can be said that esophagastroduodenoscopy with a rapid urease histochemical test for Helicobacter pylori and standard diagnostic biopsy is the optimal method for diagnosing the pathology of the upper sections of the gastrointestinal tract. Moreover, 13C-UDT seems to be almost ideal as a screening technique and for evaluating treatment in cases where endoscopic monitoring is not necessary.

Helicobacter pylori infection in children: an overview of diagnostic methods

2019 ◽  
Vol 38 (6) ◽  
pp. 1035-1045 ◽  
Author(s):  
Parisa Sabbagh ◽  
Mostafa Javanian ◽  
Veerendra Koppolu ◽  
VeneelaKrishna Rekha Vasigala ◽  
Soheil Ebrahimpour
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection

scholarly journals P-161 The diagnostic methods for detection of Helicobacter pylori infection: A comparative approach to invasive methods

2021 ◽  
Vol 32 ◽  
pp. S154-S155
Author(s):  
F. Castro-Valencia ◽  
A. Guevara-Tique ◽  
J. Suarez Olaya ◽  
A. Herrera Medina ◽  
P. Bedoya Trujillo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Comparative Approach

scholarly journals Evaluation of invasive and non-invasive methods for the diagnosis of Helicobacter pylori infection in symptomatic children and adolescents

2001 ◽  
Vol 119 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Silvio Kazuo Ogata ◽  
Elisabete Kawakami ◽  
Francy Reis Silva Patrício ◽  
Margareth Zabeu Pedroso ◽  
Antonio Mario Santos
Keyword(s):  
Helicobacter Pylori ◽  
Children And Adolescents ◽  
Breath Test ◽  
Urea Breath Test ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Predictive Values ◽  
Non Invasive ◽  
Urease Test

CONTEXT: Multiple diagnostic methods are available for the detection of Helicobacter pylori infection, but at present no single one can be used as the gold standard. OBJECTIVE: The aim of this study was to evaluate the diagnostic accuracy of 3 invasive and 2 non-invasive methods for detection of Helicobacter pylori infection in symptomatic children and adolescents. DESIGN: Prospective cohort study SETTING: Peptic Disease outpatients service, Discipline of Pediatric Gastroenterology, Universidade Federal de São Paulo / Escola Paulista de Medicina. PATIENTS: Forty-seven patients who underwent endoscopy because of dyspeptic symptoms. DIAGNOSTIC METHODS: Endoscopy with gastric biopsies for 3 invasive (rapid urease test, histology and culture) and 2 non-invasive methods (a commercial ELISA serology and 13carbon urea breath test - isotope ratio mass spectrometry) for detection of Helicobacter pylori infection. MAIN MEASUREMENTS: Sensitivity, specificity, positive and negative predictive values of each method and agreement and disagreement rates between the methods. RESULTS: Forty-seven patients [mean age, 11y9mo (SD 2y10mo), 27 female and 20 male]; 62% of them were Helicobacter pylori-positive. All methods agreed in 61%, and were negative in 21% and positive in 40%. The greatest concordance between 2 methods occurred between the invasive methods: histology and rapid urease test (89.6%) and histology and culture (87.5%). The greatest sensitivity, considering Helicobacter pylori-positive cases, for any combination of 3 or more tests, was achieved by the rapid urease test (S=100%), followed by histology, serology and 13carbon-urea breath test (S=93.1%) and lastly by culture (S=79.3%). The highest specificity was obtained by histology (100%) and culture (100%), followed by the rapid urease test (84.2%), serology (78.9%) and 13carbon-urea breath test (78.9%). CONCLUSIONS: Our results suggest that among invasive methods, an association between the rapid urease test and histology constituted the best choice for the detection of Helicobacter pylori infection. If results of histology and the rapid urease test are different, serology may be recommended.

scholarly journals European registry Helicobacter pylori (Hp-EuReg): how has clinical practice changed in Russia from 2013 to 2018 years

2019 ◽  
Vol 91 (2) ◽  
pp. 16-24 ◽  
Author(s):  
D S Bordin ◽  
Yu V Embutnieks ◽  
L G Vologzhanina ◽  
T A Ilchishina ◽  
I N Voynovan ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Practice ◽  
Serological Test ◽  
Eradication Therapy ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori ◽  
European Registry

The multicenter prospective observational study initiated by the European Helicobacter and Microbiota Study Group (EHMSG) is conducted in 27 countries in Europe. The data from the Russian part of the European registry for the management of Helicobacter pylori infection (European Registry on the management of Helicobacter pylori infection, protocol: “Hp-EuReg”) allows us to analyze the real clinical practice of diagnosis and treatment of H. pylori and compare it with international recommendations. Materials and methods. A comparative analysis of the data entered in the register by the Russian research centers “Hp-EuReg”, in the period from 2013 to 2018, was conducted. Results and discussion. Invasive diagnostic methods prevail for the primary diagnosis of H. pylori [histology - 20.3% (in 2013 year) - 43.9% (in 2018 year), rapid urease test - 31.7% and 47.8% respectively]. The most popular mode of eradication therapy is a 10-day triple therapy (62.8-76.2%), the effectiveness of which does not exceed 79% (per protocol). Invasive tests (histology) are the leading method for control the effectiveness of therapy, however, there is a tendency towards a wider use of non-invasive methods (H. pylori stool antigen - from 17% in 2013 to 29.3% in 2018 and urea breath test from 6.9 to 18.3%, respectively). Serological test to control the effectiveness of eradication is still used from 8.2% (2013) to 6.1% (2018). Eradication therapy was not performed in 28% of patients throughout the entire observation period. Conclusion. In Russia, despite approved domestic and international recommendations, deviations in clinical practice persist, both during eradication therapy and in monitoring the effectiveness of eradication therapy.

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