scholarly journals Implementation of multiplex PCR diagnostics for gastrointestinal pathogens linked to increase of notified Shiga toxin-producing Escherichia coli cases in Norway, 2007–2017

Author(s):  
Gaute Reier Jenssen ◽  
Lamprini Veneti ◽  
Heidi Lange ◽  
Line Vold ◽  
Umaer Naseer ◽  
...  
2016 ◽  
Vol 31 (2) ◽  
pp. 40-44
Author(s):  
Jehan Daoud ◽  
Karmi Mohamed ◽  
Soad Nasef ◽  
Reham Ahmed

2021 ◽  
pp. 103926
Author(s):  
Omar Hernández Hernández ◽  
Ana L. Gutiérrez-Escolano ◽  
Cleo Cancio-Lonches ◽  
Montserrat H. Iturriaga ◽  
Juan Ramiro Pacheco-Aguilar ◽  
...  

2012 ◽  
Vol 50 (5) ◽  
pp. 1752-1754 ◽  
Author(s):  
W. Zhang ◽  
M. Bielaszewska ◽  
A. Bauwens ◽  
A. Fruth ◽  
A. Mellmann ◽  
...  

2018 ◽  
Vol 9 ◽  
Author(s):  
Matthias Kiel ◽  
Pierre Sagory-Zalkind ◽  
Céline Miganeh ◽  
Christoph Stork ◽  
Andreas Leimbach ◽  
...  

2017 ◽  
Vol 63 ◽  
pp. 228-238 ◽  
Author(s):  
Kaushi S.T. Kanankege ◽  
Kelly S. Anklam ◽  
Catherine M. Fick ◽  
Megan J. Kulow ◽  
Charles W. Kaspar ◽  
...  

2011 ◽  
Vol 74 (2) ◽  
pp. 228-239 ◽  
Author(s):  
ANGELA M. VALADEZ ◽  
CHITRITA DEBROY ◽  
EDWARD DUDLEY ◽  
CATHERINE N. CUTTER

Numerous foodborne outbreaks are attributed to Shiga toxin–producing Escherichia coli (STEC) and have been recognized for causing gastrointestinal disease in humans. Beef products have been considered the principal source of STEC. A multiplex PCR assay enabling simultaneous detection of STEC O103, O91, O113, O145, O111, O157, and O26 was developed and evaluated in artificially contaminated beef carcass swabs, beef trim, and ground beef after overnight enrichment. Individual serogroups were experimentally inoculated at low (1 to 10 CFU/ml) and high (11 to 100 CFU/ml) levels, and with a cocktail of strains belonging to two, four, and six serogroups. There was no significant difference in detecting single STEC strains under the different conditions. Only when strains were combined were there significant differences in detection of all cocktail isolates in some of the beef products. To address this issue, four serogroups were experimentally inoculated together at three different estimated levels (10, 102, and 103 CFU/ml) in all three beef products. Results yielded no significant difference in detecting STEC at the three inoculation levels (10, 102, and 103 CFU/ml) in trim and carcass swabs, but there was a significant difference in detecting STEC at the lowest levels (10 and 102 CFU/ml) in the 80:20 nonirradiated ground beef, and in the detection of STECin irradiated ground beef. The findings from this study could provide industry and government agencies with a tool to evaluate the prevalence and incidence of STEC in beef products and their processing environments.


2014 ◽  
Vol 40 ◽  
pp. 31-40 ◽  
Author(s):  
Insook Son ◽  
Rachel Binet ◽  
Anna Maounounen-Laasri ◽  
Andrew Lin ◽  
Thomas S. Hammack ◽  
...  

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