Species-Specific Detection and Quantification of Toxic Marine Dinoflagellates Alexandrium tamarense and A. catenella byReal-Time PCR Assay

2005 ◽  
Vol 7 (5) ◽  
pp. 506-514 ◽  
Author(s):  
Shoko Hosoi-Tanabe ◽  
Yoshihiko Sako
Keyword(s):  
Alexandrium Tamarense ◽  
Specific Detection ◽  
Pcr Assay ◽  
Marine Dinoflagellates ◽  
Species Specific ◽  
Detection And Quantification

Development of a TaqMan PCR assay for specific detection and quantification of Pectobacterium brasiliense in potato tubers and soil

2020 ◽  
Vol 158 (2) ◽  
pp. 521-532 ◽  
Author(s):  
N. Muzhinji ◽  
J. P. Dube ◽  
E. G. de Haan ◽  
J. W. Woodhall ◽  
J. E. van der Waals
Keyword(s):  
Potato Tubers ◽  
Specific Detection ◽  
Pcr Assay ◽  
Taqman Pcr ◽  
Detection And Quantification

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

2020 ◽  
Author(s):  
Carlos Aguirre ◽  
Evelyn Sánchez ◽  
Natalia Olivares ◽  
Patricio Hinrichsen
Keyword(s):  
Real Time ◽  
Citrus Fruit ◽  
Sensitive Detection ◽  
Morphological Identification ◽  
Specific Detection ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
Cost Efficient ◽  
Species Specific ◽  
Template Dna

Abstract Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

scholarly journals Erratum to: Species-Specific Detection of Mycosphaerella polygoni-cuspidati as a Biological Control Agent for Fallopia japonica by PCR Assay

2017 ◽  
Vol 59 (9-10) ◽  
pp. 458-458
Author(s):  
Daisuke Kurose ◽  
Naruto Furuya ◽  
Tetsuya Saeki ◽  
Kenichi Tsuchiya ◽  
Seiya Tsushima ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Control Agent ◽  
Specific Detection ◽  
Pcr Assay ◽  
Fallopia Japonica ◽  
Species Specific
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