Multiplex PCR methods for the species-specific detection and quantification of Prymnesium parvum (Haptophyta)

2010 ◽  
Vol 22 (5) ◽  
pp. 587-597 ◽  
Author(s):  
Schonna R. Manning ◽  
John W. La Claire
Keyword(s):  
Multiplex Pcr ◽  
Prymnesium Parvum ◽  
Specific Detection ◽  
Species Specific ◽  
Detection And Quantification

Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea

2019 ◽  
Vol 510 ◽  
pp. 31-45 ◽  
Author(s):  
Steen Wilhelm Knudsen ◽  
Rasmus Bach Ebert ◽  
Martin Hesselsøe ◽  
Franziska Kuntke ◽  
Jakob Hassingboe ◽  
...  
Keyword(s):  
Baltic Sea ◽  
Environmental Dna ◽  
Marine Fishes ◽  
Specific Detection ◽  
The Baltic Sea ◽  
The Baltic ◽  
Species Specific ◽  
Detection And Quantification

Species-specific detection and quantification of common barnacle larvae from the Japanese coast using quantitative real-time PCR

Biofouling ◽  
2010 ◽  
Vol 26 (8) ◽  
pp. 901-911 ◽  
Author(s):  
Noriyuki Endo ◽  
Kana Sato ◽  
Kiyotaka Matsumura ◽  
Erina Yoshimura ◽  
Yukiko Odaka ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Specific Detection ◽  
Japanese Coast ◽  
Species Specific ◽  
Detection And Quantification

scholarly journals Detection and Differentiation ofListeria spp. by a Single Reaction Based on Multiplex PCR

1999 ◽  
Vol 65 (10) ◽  
pp. 4688-4692 ◽  
Author(s):  
Andreas Bubert ◽  
Inge Hein ◽  
Marcus Rauch ◽  
Angelika Lehner ◽  
ByoungSu Yoon ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Sequences ◽  
Simultaneous Detection ◽  
Specific Gene ◽  
Food Hygiene ◽  
Specific Detection ◽  
Pcr Method ◽  
Species Specific ◽  
Different Sources ◽  
Single Reaction

ABSTRACT The iap gene encodes the protein p60, which is common to all Listeria species. A previous comparison of the DNA sequences indicated conserved and species-specific gene portions. Based on these comparisons, a combination consisting of only five different primers that allows the specific detection and differentiation ofListeria species with a single multiplex PCR and subsequent gel analysis was selected. One primer was derived from the conserved 3′ end and is specific for all Listeria species; the other four primers are specific for Listeria monocytogenes,L. innocua, L. grayi, or the three grouped species L. ivanovii, L. seeligeri, and L. welshimeri, respectively. The PCR method, which also enables the simultaneous detection of L. monocytogenes and L. innocua, was evaluated against conventional biotyping with 200 food hygiene-relevant Listeria strains. The results indicated the superiority of this technique. Thus, this novel type of multiplex PCR may be useful for rapid Listeria species confirmation and for identification of Listeria species for strains isolated from different sources.

64: Rapid, Species-Specific Detection of Uropathogens from Clinical Urine Specimens Using an Electrochemical Microsensor Array

2005 ◽  
Vol 173 (4S) ◽  
pp. 18-18
Author(s):  
Joseph C. Liao ◽  
Mitra Mastali ◽  
David A. Haake ◽  
Bernard M. Churchill
Keyword(s):  
Specific Detection ◽  
Species Specific ◽  
Urine Specimens
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