Prolonged preservation of rat peritoneal mesothelial cells (RPMC) and rat peritoneal fibroblasts (RPFB) with neutral pH peritoneal dialysis solution depleted of glucose degradation products (GDPs)

2001 ◽  
Vol 5 (4) ◽  
pp. 257-264 ◽  
Author(s):  
T. Horiuchi ◽  
T. Inoue ◽  
K. Nagasawa
Keyword(s):  
Peritoneal Dialysis ◽  
Degradation Products ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Neutral Ph ◽  
Glucose Degradation Products ◽  
Dialysis Solution ◽  
Peritoneal Dialysis Solution

scholarly journals PPAR-γ agonist rosiglitazone protects rat peritoneal mesothelial cells against peritoneal dialysis solution-induced damage

2017 ◽  
Vol 15 (4) ◽  
pp. 1786-1792 ◽  
Author(s):  
Yun-Fang Zhang ◽  
Qi Wang ◽  
Yan-Yan Su ◽  
Jie-Lin Wang ◽  
Bao-Jun Hua ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Ppar Γ ◽  
Dialysis Solution ◽  
Peritoneal Dialysis Solution

scholarly journals Glucose degradation products in neutralized peritoneal dialysis solution

2004 ◽  
Vol 37 (12) ◽  
pp. 2069-2077 ◽  
Author(s):  
Tadashi Yamamoto ◽  
Tsuyoshi Izumotani ◽  
Senji Qkuno ◽  
Tomoyuki Yamakawa
Keyword(s):  
Peritoneal Dialysis ◽  
Degradation Products ◽  
Glucose Degradation Products ◽  
Dialysis Solution ◽  
Peritoneal Dialysis Solution

scholarly journals Prolonged Exposure to Glucose Degradation Products Impairs Viability and Function of Human Peritoneal Mesothelial Cells

2001 ◽  
Vol 12 (11) ◽  
pp. 2434-2441 ◽  
Author(s):  
JANUSZ WITOWSKI ◽  
JUSTYNA WISNIEWSKA ◽  
KATARZYNA KORYBALSKA ◽  
THORSTEN O. BENDER ◽  
ANDRZEJ BREBOROWICZ ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Cell Viability ◽  
Degradation Products ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Glucose Degradation Products ◽  
Human Peritoneal Mesothelial Cells ◽  
And Function ◽  
The Impact

Abstract. Bioincompatibility of peritoneal dialysis fluids (PDF) has been linked to the presence of glucose degradation products (GDP). Previous experiments have shown that short-term exposure to several GDP at concentrations found in commercially available PDF had no significant effect on human peritoneal mesothelial cells (HPMC). During continuous ambulatory peritoneal dialysis, however, cells are continually exposed to GDP for extended periods of time. Thus, the impact of GDP on HPMC during long-term exposure was assessed. HPMC were cultured for up to 36 d in the presence of 6 identified GDP (acetaldehyde, formaldehyde, furaldehyde, glyoxal, methylglyoxal, and 5-HMF) at doses that reflect their concentrations in conventional PDF. At regular time intervals, the ability of HPMC to secrete cytokines (interleukin-6 [IL-6]) and extracellular matrix molecules (fibronectin) was evaluated. In addition, cell viability, morphology, and proliferative potential were assessed. Exposure to GDP resulted in a significant reduction in mesothelial IL-6 and fibronectin release. Approximately 80% of this decrease occurred during the first 12 d of the exposure and was paralleled by a gradual loss of cell viability and development of morphologic alterations. After 36 d of exposure, the number of cells in GDP-treated cultures was reduced by nearly 60%. However, GDP-treated cells were able to resume normal proliferation when transferred to a normal GDP-free medium. HPMC viability and function may be impaired during long-term exposure to clinically relevant concentrations of GDP, which suggests a potential role of GDP in the pathogenesis of peritoneal membrane dysfunction during chronic peritoneal dialysis.

scholarly journals Cultured rat mesothelial cells generate hydrogen peroxide: a new player in peritoneal defense?

1996 ◽  
Vol 7 (11) ◽  
pp. 2371-2378
Author(s):  
A Shostak ◽  
E Pivnik ◽  
L Gotloib
Keyword(s):  
Hydrogen Peroxide ◽  
Peritoneal Dialysis ◽  
Phorbol Myristate Acetate ◽  
Mesothelial Cells ◽  
Spontaneous Release ◽  
Myristate Acetate ◽  
Peritoneal Mesothelial Cells ◽  
Dialysis Solution ◽  
Peritoneal Dialysis Solution ◽  
Hydrogen Peroxide Generation

This study was designed to examine whether rat peritoneal mesothelial cells in culture could generate hydrogen peroxide in different experimental conditions. Mesothelial cells, incubated in M-199, spontaneously released hydrogen peroxide. This process was significantly increased by addition of phorbol myristate acetate, as well as of superoxide dismutase to the medium, whereas it was substantially inhibited by catalase. Exposure of mesothelial cells to modified M-199 medium with 1.5% glucose concentration-lactated peritoneal dialysis solution did not seem to interfere either with the spontaneous release of hydrogen peroxide, or with that induced by phorbol myristate acetate. Furthermore, exposure of mesothelial cells to the glucose (4.25%) peritoneal dialysis solution in Medium M-199, was coincident with increased hydrogen peroxide generation, which was significantly higher than the spontaneous release, and not far from that observed with phorbol myristate acetate and superoxide dismutase. So far, it can be inferred from this evidence that peritoneal mesothelial cells in culture are not only endowed with the capability of producing hydrogen peroxide, but they can also be activated to do so in a way comparable to that observed in neutrophils and macrophages. This attribute is one more indication that mesothelial cells play a relevant role in the peritoneal mechanism of defense against infection. On the other hand, continuous exposure of mesothelial cells to glucose-enriched fluids, as occurs in clinical continuous ambulatory peritoneal dialysis, may well also be at the origin of a process of continuous injury, resulting from an increased hydrogen peroxide generation.

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