Influence of Magnetic Fields with Induction of 7 T on Physical and Chemical Properties of Aqueous NaCl Solutions

The physicochemical properties of NaCl aqueous solutions in a wide range of concentrations were studied. We determined that constant magnetic fields with an induction of up to 7 T had a significant effect on the physicochemical properties of these solutions. First, we detected a decrease in pH that was dependent on the magnetic field strength both in water and in NaCl solutions. This effect was not associated with the presence of sodium cations or chlorine anions in water. Secondly, with an increase in magnetic field induction the redox potential of aqueous solutions also increased. Magnetic fields did not cause any changes in the concentration of dissolved molecular oxygen in deionized water. In this case, in aqueous solutions of NaCl under the action of a magnetic field, a concentration-dependent tendency to a decrease in the concentration of dissolved molecular oxygen is observed. Third, it was shown that under the action of a magnetic field on a NaCl solution, the rate of hydrogen peroxide generation increased with increasing NaCl concentration. Fourth, the essential role of the primary state of aqueous solutions in relation to the gas composition and gas equilibrium under magnetic influence was established. The work also evaluated the contribution of flow-mixing during sample preparation on the physicochemical properties of the solutions.

Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness

Hypoxic microenvironment is common in solid tumors, particularly in pancreatic ductal adenocarcinoma (PDAC). The Warburg effect is known to facilitate cancer aggressiveness and has long been linked to hypoxia, yet the underlying mechanism remains largely unknown. In this study, we identify that lysyl oxidase-like 2 (LOXL2) is a hypoxia-responsive gene and is essential for the Warburg effect in PDAC. LOXL2 stabilizes hypoxia-inducible factor 1α (HIF1α) from prolyl hydroxylase (PHD)-dependent hydroxylation via hydrogen peroxide generation, thereby facilitating the transcription of multiple glycolytic genes. Therefore, a positive feedback loop exists between LOXL2 and HIF1α that facilitates glycolytic metabolism under hypoxia. Moreover, LOXL2 couples the Warburg effect to tumor growth and metastasis in PDAC. Hijacking glycolysis largely compromises LOXL2-induced oncogenic activities. Collectively, our results identify a hitherto unknown hypoxia-LOXL2-HIF1α axis in regulating the Warburg effect and provide an intriguing drug target for PDAC therapy.