scholarly journals Early development of the self-fertilizing mangrove killifish Rivulus marmoratus reared in the laboratory

2004 ◽  
Vol 51 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Maria Vivian Camacho Grageda ◽  
Yoshitaka Sakakura ◽  
Atsushi Hagiwara
Keyword(s):  
Early Development ◽  
The Self ◽  
Rivulus Marmoratus ◽  
Mangrove Killifish

scholarly journals Genetic and growth differences in the outcrossings between two clonal strains of the self-fertilizing mangrove killifish

2008 ◽  
Vol 86 (9) ◽  
pp. 976-982 ◽  
Author(s):  
Yukino Nakamura ◽  
Koushirou Suga ◽  
Yoshitaka Sakakura ◽  
Takashi Sakamoto ◽  
Atsushi Hagiwara
Keyword(s):  
Artificial Insemination ◽  
Genetic Architecture ◽  
Life History Traits ◽  
Fragment Length Polymorphism ◽  
The Self ◽  
Aflp Analysis ◽  
Rivulus Marmoratus ◽  
Kryptolebias Marmoratus ◽  
Growth Differences ◽  
Mangrove Killifish

The populations of the only known self-fertilizing vertebrate Kryptolebias marmoratus (Poey, 1880) (formerly known as Rivulus marmoratus Poey, 1880; Cyprinodontiformes: Aplocheilidae) usually consist of different homozygous lineages; however, heterozygous individuals are found occasionally and ratios of homozygosity and heterozygosity in populations are dependent on the proportion of males. However, it is still unclear what impact male-mediated genetic diversity has on the phenotype of K. marmoratus. To clarify this, we attempted outcrossing between male and hermaphrodite of two different clonal strains with different life-history traits using artificial insemination, and examined the genotypes and growth of the hybrid F2 generation. We detected genetic differences between the two clonal strains using amplified fragment length polymorphism (AFLP) analysis with 3 primer combinations, and then obtained 11 AFLP markers. From a total of 31 artificial inseminations with two clonal strains, 1 of 13 hatched fish clearly indicated heterozygosity. The hybrid F2 generations were also heterozygous. Moreover, the growths of the hybrid F2 generation were intermediate of the parental strains from days 0 to 30. Therefore, outcrossing changes genetic architecture and the new genotypes potentially result in new phenotypes of the subsequent generations of K. marmoratus. It may also play a role in adaptation to new environments and the facilitation of local adaptation.

Extreme vertebral variation induced by temperature in a homozygous clone of the self-fertilizing cyprinodontid fish Rivulus marmoratus

1972 ◽  
Vol 50 (6) ◽  
pp. 733-744 ◽  
Author(s):  
C. C. Lindsey ◽  
Robert W. Harrington Jr.
Keyword(s):  
Early Development ◽  
Gene Pool ◽  
The Self ◽  
Selective Mortality ◽  
Genetic Uniformity ◽  
Rivulus Marmoratus ◽  
Meristic Variation ◽  
Retinal Pigmentation ◽  
High Degree ◽  
Hatching Temperature

Embryos from one clone of Rivulus marmoratus were reared at various constant temperatures ranging from 19.5 to 31.2C, sustained throughout early development, or else were transferred from 26 to 20C after various periods of development. Resultant vertebral counts were progressively lower at higher sustained temperatures (ranging from 34.94 to 32.57). Vertebral differences expressed as a percentage of the total count were greater in this (7.0%) than in previously reported comparable experiments on 14 other fish species (4.1% or less). In most previous experiments meristic variation may have been due, at least in part, to selective mortality acting on a varied gene pool. In R. marmoratus the high degree of genetic uniformity eliminates the likelihood of selective mortality as a cause of vertebral variation, which must therefore have been environmentally induced. Pectoral ray counts also were lower at higher sustained temperatures; other meristic series did not show sharp responses. Temperature transfer experiments showed that vertebral counts are determined within 4 days at 26C (by first appearance of retinal pigmentation). Pectoral ray counts are not fully determined until about 8 days, shortly before hatching. Temperature breaks produced "shock effect" in pectoral rays but not in vertebrae. The bearing of homozygosity on amplitude of temperature-induced vertebral variation is discussed.

Introduction to Volume 8

2016 ◽  
pp. 3-30
Author(s):  
Ann Horne
Keyword(s):  
Early Development ◽  
Scientific Method ◽  
The Self ◽  
Real Object ◽  
Patient Relationship ◽  
Key Concepts ◽  
Use Of An Object

The Introduction to Volume 8 elicits the key concepts Winnicott was developing in 1967-68, beginning in January 1967 with his talk on his own theories of development at the ‘52 Club, citing those who influenced him and those from whom he acquired ideas. The author proceeds to examine ‘The Use of an Object’, viewed by Claire Winnicott as the culmination of his thinking, a talk given at the NYPSI in November 1968. There is comment on Winnicott’s approach to observation and the scientific method, on culture and playing, and a fuller picture of friends and interests from the 1967 IPA conference. Failures in early development are explored but the main focus is the emergence of the self as real and the recognition of a real object that can be used (from ‘Mirror-role of mother and family’ to ‘The use of an object’) and the parallel between the early mother-infant relationship and the analyst-patient relationship. Thoughts on technique conclude the paper.

Influence of reproductive history of parents on meristic variation in offspring in the cyprinodont fish Rivulus marmoratus

1986 ◽  
Vol 64 (7) ◽  
pp. 1456-1459 ◽  
Author(s):  
D. P. Swain ◽  
C. C. Lindsey
Keyword(s):  
Reproductive History ◽  
The Self ◽  
Caudal Fin ◽  
Rivulus Marmoratus ◽  
Fin Rays ◽  
History Of ◽  
Meristic Variation

Parent fish from one clone of the self-fertilizing cyprinodont fish Rivulus marmoratus were held under constant conditions, and their offspring were examined for meristic variation associated with their position in the oviposition sequence of parents. In all meristic series counted (vertebrae, and anal, dorsal, pectoral, and caudal fin rays) those offspring produced soon after their parents had begun to lay eggs tended to have fewer parts than did those produced longer after the onset of oviposition. Most meristic differences were due to the low counts produced in embryos laid within 8 days of first oviposition; counts differed little among embryos laid at averages of 22, 86, and 158 days after first oviposition. Differences were most significant for dorsal and anal rays and not significant for caudal rays.

scholarly journals Microsatellite Documentation of Male-Mediated Outcrossing between Inbred Laboratory Strains of the Self-Fertilizing Mangrove Killifish (Kryptolebias Marmoratus)

2006 ◽  
Vol 97 (5) ◽  
pp. 508-513 ◽  
Author(s):  
Mark Mackiewicz ◽  
Andrey Tatarenkov ◽  
Andrew Perry ◽  
J. Ryce Martin ◽  
John F. Elder ◽  
...  
Keyword(s):  
The Self ◽  
Kryptolebias Marmoratus ◽  
Mangrove Killifish

scholarly journals Affects, Attunements and the Intersubjective Self: Perspectives from Early Development

2020 ◽  
Vol 31 ((1)) ◽  
Author(s):  
Stephen Seligman
Keyword(s):  
Early Development ◽  
Projective Identification ◽  
The Self ◽  
Intersubjective Self ◽  
Affect Attunement ◽  
Point Of Departure ◽  
Infant Observation

This paper conceptualizes the self and related concepts so as to emphasize interrelatedness rather than autonomy. From this view of a subject embedded in relationships as a point of departure, it then critiques and restates certain analyticallyoriented concepts so as to render them in a more fully intersubjective frame: “affect attunement” (Stern, 1985), “mirroring” (Kohut, 1977), empathy, and projective identification. This approach draws on drawing on the infant observation research that has emerged in recent decades.

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