Phylogenetic analysis of Rhizoctonia solani subgroups associated with web blight symptoms on common bean based on ITS-5.8S rDNA

Variability of 45 isolates of Rhizoctonia solani (teleomorph Thanatephorus cucumeris) causing web blight (WB) of common bean, Phaseolus vulgaris, was examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer regions (ITS1 and ITS2) and the 5.8S subunit (5.8S) of the nuclear ribosomal DNA repeat (ITS-5.8S-rDNA). Isolates were collected from diseased bean leaves from Argentina, Costa Rica, Cuba, Dominican Republic, Honduras, Panama, and Puerto Rico. These WB isolates belong to AG-1 and AG-2 based on anastomosis reaction. Isolates of AG-1 that cause WB were separated into three distinct groups of RFLP patterns from enzymatic digestion of a 740-bp PCR fragment. Microsclerotia-producing isolates (<1 mm) were differentiated from macrosclerotia-producing isolates (5 to 20 mm) based on PCR-RFLP patterns even though they are placed in the same AG1-1B subgroup by anastomosis reaction. WB isolates of AG-2 were separated into two distinct PCR-RFLP groups as previously reported. AG-1 macrosclerotial-producing isolates were the most virulent, whereas isolates of AG-2 were the least virulent. Genetic variability of the WB pathogen may have influenced the failure or success of management practices implemented in the past in Latin America.

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Isolates of Rhizoctonia solani Can Produce both Web Blight and Root Rot Symptoms in Common Bean (Phaseolus vulgaris L.)

Plant Disease ◽

10.1094/pdis-11-15-1270-re ◽

2016 ◽

Vol 100 (7) ◽

pp. 1351-1357 ◽

Cited By ~ 8

Author(s):

Suheidy Valentín Torres ◽

María M. Vargas ◽

Graciela Godoy-Lutz ◽

Timothy G. Porch ◽

James S. Beaver

Keyword(s):

Phaseolus Vulgaris ◽

Rhizoctonia Solani ◽

Common Bean ◽

Root Rot ◽

Control Strategies ◽

Limited Information ◽

Phaseolus Vulgaris L ◽

Planting Dates ◽

Significant Line ◽

Web Blight

In common bean (Phaseolus vulgaris L.), Rhizoctonia solani Kühn is an important pathogen causing web blight (WB) in the tropics, and it is also a soilborne pathogen causing root rot (RR) worldwide. This pathogen is a species complex classified into 14 anastomosis groups (AG). AG 1-IA, AG 1-IB, AG 1-IE, AG 1-IF, AG 2-2, and AG 4 have been reported to cause WB of the aboveground structures of the plant, while AG 4 and AG 2-2 have been associated with RR. There is limited information, however, concerning the ability of particular isolates of specific AG to cause both diseases in common bean. Nine R. solani isolates, including three AG 1 and three AG 4 WB isolates and three AG 4 RR isolates collected from both leaves and roots, respectively, of common bean in Puerto Rico, were used to evaluate the response of 12 common bean genotypes to WB inoculated using a detached-leaf method and to RR inoculated using a solution suspension of R. solani mycelia in the greenhouse. All R. solani isolates were able to induce both RR and WB symptoms. RR readings were generally more severe than the WB readings. The RR isolate RR1 (AG 4) produced the most severe RR scores. A few bean lines had mean RR scores ≤4.4 for specific R. solani isolates on a scale of 1 to 9, with 1 representing resistant and 9 highly susceptible. However, all of the bean lines had mean RR scores ≥5.0 when inoculated with the isolates RR1, RR2, and RR3, which were determined to be AG 4 in this study. Significant line–isolate interactions were observed for the WB and RR inoculations for the three planting dates, suggesting a differential response of the common bean lines to the pathogen. This genotypic interaction may require bean breeders and pathologists to monitor the virulence patterns of R. solani in specific growing environments, while the compatibility of specific R. solani isolates to both aerial and root tissue needs to be considered for disease control strategies.

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Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina

Journal of Phytopathology ◽

10.1111/jph.12526 ◽

2016 ◽

Vol 164 (11-12) ◽

pp. 1054-1063 ◽

Cited By ~ 2

Author(s):

Yamila Spedaletti ◽

Mónica Aparicio ◽

Guadalupe Mercado Cárdenas ◽

Marcela Rodriguero ◽

Gisel Taboada ◽

...

Keyword(s):

Rhizoctonia Solani ◽

Common Bean ◽

Genetic Characterization ◽

Web Blight

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VIRULENCE OF RHIZOCTONIA SOLANI ISOLATES ASSOCIATED WITH WEB BLIGHT OF COMMON BEAN

The Journal of Agriculture of the University of Puerto Rico ◽

10.46429/jaupr.v84i1-2.3906 ◽

1969 ◽

Vol 84 (1-2) ◽

pp. 95-97

Author(s):

Manuel de J. Bautista-Pérez ◽

Rodrigo Echávez-Badel ◽

Raúl E. Macchiavelli

Keyword(s):

Rhizoctonia Solani ◽

Common Bean ◽

Web Blight

VIRULENCE OF RHIZOCTONIA SOLANI ISOLATES ASSOCIATED WITH WEB BLIGHT OF COMMON BEAN

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The Use of Trichoderma koningii in the Control of Web Blight Disease Caused by Rhizoctonia solani in the Foliage of Cowpea (Vigna unguiculata)

Journal of Phytopathology ◽

10.1111/j.1439-0434.1991.tb00159.x ◽

1991 ◽

Vol 133 (3) ◽

pp. 247-254 ◽

Cited By ~ 5

Author(s):

A. O. Latunde-Dada

Keyword(s):

Rhizoctonia Solani ◽

Vigna Unguiculata ◽

Trichoderma Koningii ◽

Blight Disease ◽

Web Blight

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Reactions of Some Common Bean Cultivars to Rhizoctonia Web Blight

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi ◽

10.24180/ijaws.578054 ◽

2019 ◽

Vol 5 (2) ◽

pp. 273-279

Author(s):

Gülsüm Palacıoğlu ◽

Harun Bayraktar ◽

Göksel Özer

Keyword(s):

Common Bean ◽

Web Blight

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Rhizoctonia solani AG-1 IA infects both rice and signalgrass in the Colombian Llanos

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632016v4638696 ◽

2016 ◽

Vol 46 (1) ◽

pp. 65-71 ◽

Cited By ~ 3

Author(s):

Lina Maria Ramos-Molina ◽

Edisson Chavarro-Mesa ◽

Danilo Augusto dos Santos Pereira ◽

María del Rosario Silva-Herrera ◽

Paulo Cezar Ceresini

Keyword(s):

Rhizoctonia Solani ◽

Species Complex ◽

Molecular Detection ◽

Sheath Blight ◽

Pathogenicity Test ◽

Rice Sheath Blight ◽

Specific Primers ◽

5.8S Rdna ◽

Foliar Blight ◽

Rhizoctonia Oryzae

ABSTRACT Foliar blight and death of signalgrass (Urochloa spp.) pastures are caused by the Rhizoctonia solani fungus. This study aimed at determining which pathogens from the Rhizoctonia species complex are associated with leaf and sheath blight in Urochloa and rice, in the Colombian Llanos. Sympatric areas of Urochloa pastures adjacent to rice cropping areas were sampled using a linear transect system. The pathogens were identified using morphological traits, molecular detection based on specific primers and sequencing of the ITS-5.8S rDNA region. R. solani AG-1 IA predominated as the pathogen associated with foliar blight in all samples from U. brizantha cv. 'Toledo' and hybrid Urochloa cv. 'Mulato'. Besides R. solani AG-1 IA (18 % of the samples), Rhizoctonia oryzae-sativae (71 %) and Sclerotium hydrophilum (11 %) were also detected. In the cross-pathogenicity test, the R. solani AG-1 IA fungus was the most aggressive to Urochloa, while R. oryzae-sativae produced very mild infection symptoms. This is the first report of R. oryzae-sativae and S. hydrophilum associated with the complex of rice sheath blight diseases in Colombia.

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Root-rot resistance in common bean

Canadian Journal of Plant Science ◽

10.4141/cjps93-054 ◽

1993 ◽

Vol 73 (1) ◽

pp. 365-367 ◽

Cited By ~ 11

Author(s):

J. C. Tu ◽

S. J. Park

Keyword(s):

Phaseolus Vulgaris ◽

Rhizoctonia Solani ◽

Common Bean ◽

Fusarium Oxysporum ◽

Key Words ◽

Fusarium Solani ◽

Root Rot ◽

Pythium Ultimum ◽

Growth Room

A bean (Phaseolus vulgaris) line, A - 300, resistant to Rhizoctonia solani and Fusarium oxysporum was introduced into Ontario from Colombia. The results of tests conducted in a root-rot nursery, in a greenhouse and in a growth room showed that this bean line is resistant to Fusarium solani f. sp. phaseoli and Pythium ultimum. Key words: Bean, Phaseolus vulgaris, root rot resistance

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