Immobilized Kallikrein Microreactor Based on Capillary Electrophoresis for Online Enzyme Kinetics Analysis and Inhibitor Screening

A novel online α-glucosidase-immobilized microreactor was developed by immobilizing α-glucosidase on capillary inner wall. The microreactor combination with capillary electrophoresis was applied in studying enzyme kinetics and inhibition kinetics.

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Application of robotics to steady state enzyme kinetics: analysis of tight-binding inhibitors of dipeptidyl peptidase IV

Analytical Biochemistry ◽

10.1016/s0003-2697(03)00434-2 ◽

2003 ◽

Vol 321 (2) ◽

pp. 157-166 ◽

Cited By ~ 5

Author(s):

Aiying Wang ◽

Yanting Huang ◽

Prakash Taunk ◽

David R Magnin ◽

Krishnendu Ghosh ◽

...

Keyword(s):

Steady State ◽

Enzyme Kinetics ◽

Tight Binding ◽

Dipeptidyl Peptidase ◽

Dipeptidyl Peptidase Iv ◽

Kinetics Analysis ◽

Binding Inhibitors

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Capillary Electrophoresis with Laser-Induced Fluorescence Detection as a Tool for Enzyme Characterization and Inhibitor Screening

Analytical Sciences ◽

10.2116/analsci.24.333 ◽

2008 ◽

Vol 24 (3) ◽

pp. 333-337 ◽

Cited By ~ 4

Author(s):

Dan-Ning LIU ◽

Li LI ◽

Wei-Ping LU ◽

Yao-Qing D. LIU ◽

Ken R. WEHMEYER ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Fluorescence Detection ◽

Enzyme Characterization ◽

Laser Induced Fluorescence ◽

Inhibitor Screening ◽

Laser Induced Fluorescence Detection

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Inhibitor screening and selectivity assessment against multiple cellular protein kinases by capillary electrophoresis with laser-induced fluorescence detection

Chinese Journal of Chromatography ◽

10.3724/sp.j.1123.2013.04032 ◽

2013 ◽

Vol 31 (7) ◽

pp. 646 ◽

Cited By ~ 1

Author(s):

Qianqian ZHANG ◽

Xuepei ZHANG ◽

Hanzhi ZHANG ◽

Jingwu KANG

Keyword(s):

Capillary Electrophoresis ◽

Protein Kinases ◽

Fluorescence Detection ◽

Laser Induced Fluorescence ◽

Cellular Protein ◽

Inhibitor Screening ◽

Laser Induced Fluorescence Detection

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Immobilized capillary tyrosinase microreactor for inhibitor screening in natural extracts by capillary electrophoresis

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2013.05.023 ◽

2013 ◽

Vol 84 ◽

pp. 36-40 ◽

Cited By ~ 26

Author(s):

Ting-Fu Jiang ◽

Ting-Ting Liang ◽

Yuan-Hong Wang ◽

Wen-Hui Zhang ◽

Zhi-Hua Lv

Keyword(s):

Capillary Electrophoresis ◽

Inhibitor Screening ◽

Natural Extracts

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Preparation of Magnetic Microsphere-Gold Nanoparticle-Immobilized Enzyme Batch Reactor and Its Application to Enzyme Inhibitor Screening in Natural Extracts by Capillary Electrophoresis

Chinese Journal of Chemistry ◽

10.1002/cjoc.201600800 ◽

2017 ◽

Vol 35 (6) ◽

pp. 943-948 ◽

Cited By ~ 3

Author(s):

Hengshan Wang ◽

Pingtan Lin ◽

Shulin Zhao ◽

Shuting Li ◽

Xin Lu ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Gold Nanoparticle ◽

Immobilized Enzyme ◽

Enzyme Inhibitor ◽

Batch Reactor ◽

Magnetic Microsphere ◽

Inhibitor Screening ◽

Natural Extracts

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Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles

RSC Advances ◽

10.1039/d0ra09222c ◽

2021 ◽

Vol 11 (6) ◽

pp. 3596-3602

Author(s):

Zhen Lin ◽

Xiaohui Tan ◽

Fangping Li ◽

Yu Zhang ◽

Ping Luo ◽

...

Keyword(s):

Enzyme Activity ◽

Magnetic Nanoparticles ◽

Enzyme Kinetics ◽

Binding Protein ◽

Aldehyde Oxidase ◽

Kinetics Analysis

Superparamagnetic nanometer beads bonding SIP was employed to pull down the binding protein from the liver of mouse, which was identified as aldehyde oxidase 3. By means of enzyme kinetics analysis, SIP was found to activate AOX3 enzyme activity.

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