Genetic variability of Italian southern Scots pine (Pinus sylvestris L.) populations: the rear edge of the range

Abstract Scots pine (Pinus sylvestris L.) is the most common species in Poland’s forest stands. The mode of pine stands renovation requires that silviculture practitioners have continuous access to seed banks. Orchard-grown seeds are predicted to constitute an increasingly larger part of the average demand for pine seeds in Poland. Seed orchards, due to a limited number of maternal trees as well as the irregularity of their blooming and pollination, enhance the risk of genetic diversity reduction in planted forest stands. This is of particular importance in the context of dynamic climate change. Markers based on microsatellite DNA fragments are effective tools for monitoring genetic variability. In the present study, three different microsatellite DNA fragments were used: SPAC 12.5, SPAG 7.14 and SPAC 11.4. The main objective of this research was to study genetic variability in one of the biggest seed orchards in Poland, located in the Forest District Susz. The obtained results indicated heterozygosity loss within the orchard, proving the existence of specimen selection effects on genetic variability. Hence, it seems quite important to take account of molecular genetic variability of maternal trees in future breeding strategies.

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Genetic variability of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) natural regeneration compared with their maternal stands

Forest Research Papers ◽

10.2478/frp-2014-0005 ◽

2014 ◽

Vol 75 (1) ◽

pp. 47-54 ◽

Cited By ~ 5

Author(s):

Justyna Anna Nowakowska ◽

Tadeusz Zachara ◽

Agata Konecka

Keyword(s):

Genetic Variability ◽

Picea Abies ◽

Pinus Sylvestris ◽

Norway Spruce ◽

Scots Pine ◽

Natural Regeneration ◽

Gene Pool ◽

Inbreeding Coefficient ◽

Adult Trees ◽

Forest District

Abstract The genetic variability and biodiversity of tree populations ensure the stability and sustainability of forest ecosystems. New research tools based on molecular DNA markers enable precise characterisation of forest genetic resources, i.e. detection of different allele frequencies in mature trees and progeny populations. The paper describes the genetic structure of mature stands of Scots pine (Pinus sylvestris L.) in Oława Forest District and Norway spruce (Picea abies L. Karst.) in Jawor Forest District and stands of their respective progeny. In the Scots pine stand, there was a slight increase (0.6%) in heterozygosity level and a larger increase (4.9%) in the inbreeding coefficient of progeny trees. In the Norway spruce stand, despite a small reduction (0.9%) in heterozygosity, a similar increase (4.6%) in the inbreeding coefficient of progeny was revealed. In both stands, allele richness and the partition probability of basic clustering were high. Both pine and spruce adults and progeny trees were characterised by high levels of genetic similarity (96% and 79%, respectively). Gene flow between the mature and progeny populations was high (Nm > 1) for both Scots pine and Norway spruce. Conservation of the gene pool within forest tree stands requires an increase in the proportion of natural regeneration. To estimate the extent to which genes are transmitted between adult trees and their progeny, more studies are needed, especially taking into account the influence of silviculture measures, like selective tree cutting, on the genetic variability of the younger generation. These results confirm that the gene pool was conserved when transmitted between the stands studied, as well as highlight the usefulness of such a study for silvicultural purposes

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Genetic variability of Scots pine (Pinus sylvestris) provenances in Spain: growth traits and survival

Silva Fennica ◽

10.14214/sf.601 ◽

2001 ◽

Vol 35 (1) ◽

Cited By ~ 25

Author(s):

Ricardo Alía ◽

Javier Moro-Serrano ◽

Eduardo Notivol

Keyword(s):

Genetic Variability ◽

Pinus Sylvestris ◽

Scots Pine ◽

Growth Traits

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Analysis of inter - population variability Scots pine (Pinus sylvestris L.) using morphometric markers

Genetika ◽

10.2298/gensr1203689l ◽

2012 ◽

Vol 44 (3) ◽

pp. 689-699

Author(s):

Aleksandar Lucic ◽

Vasilije Isajev ◽

Ljubinko Rakonjac ◽

Milan Mataruga ◽

Vladan Popovic ◽

...

Keyword(s):

Genetic Variability ◽

Pinus Sylvestris ◽

Scots Pine ◽

Population Differentiation ◽

Morphometric Parameters ◽

Population Variability ◽

Biological Similarity ◽

Neck Diameter ◽

Significant Population Differentiation

The aim of this study is analysis of inter-population variability of Scots pine (Pinus sylvestris L.) using morphometric parameters of cones and seedlings originating from seven populations in Serbia.The analysis of 1960 cones and their seeds was performed as well as the analysis of morphometric parameters of seedlings (height and root neck diameter) that were produced from this seed. Based on the obtained results the significant differences were noticed in the values of analyzed characters for each population separately. The biological similarity or distance regarding the analyzed characters showed a significant population differentiation. By presenting the preliminary knowledge on the genetic variability of the study populations, the reported results will contribute to better understanding of the significance of the analyzed characters in the population differentiation.

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Genetic variability of Scots pine (Pinus sylvestris L.) in maternal regions of provenance

Folia Forestalia Polonica ◽

10.1515/ffp-2015-0011 ◽

2015 ◽

Vol 57 (2) ◽

pp. 112-119 ◽

Cited By ~ 1

Author(s):

Paweł Przybylski ◽

Jan Matras ◽

Małgorzata Sułkowska

Keyword(s):

Genetic Variability ◽

Pinus Sylvestris ◽

Scots Pine ◽

Genetic Parameters ◽

Fixation Index ◽

Effective Number ◽

Expected Number ◽

Expected Heterozygosity ◽

Rare Alleles ◽

Fixation Indices

Abstract The analysis of chosen selected Scots Pine (Pinus sylvestris L.), populations representing different seed regions: 107 (Międzyzdroje), 305 (Woziwoda), 206 (Strzałowo), 208 (Białowieża), 504 (Bolesławiec), 606 (Józefów) were performed using 10 isoensyme markers: Gdh (E.C.1.4.1.2), Sdh-A, Sdh-B (E.C.1.1.1.25), Pgd-B (E.C.1.1.1.44), Mdh-A, Mdh-C (E.C.1.1.1.37), Got-A, Got-B, Got-C (E.C.2.6.1.1), Dia-C (E.C.1.8.1.4). There were calculated following genetic parameters: allelic frequencies, observed and expected heterozygosities, and Wright’s fixation indexes. In populations, the results of analysis indicated presence of rare alleles. In all study populations, the average effective number of alleles per locus was 1.46 and was lower than expected number of alleles per locus of 1.93. The results for the effective number of alleles of the population per locus were as follows: Strzałowo and Białowieża 1.54, Bolesławiec 1.48, Jozefów 1.44, Świnoujście 1.42, Woziwoda 1.35. Average observed heterozygosity in the studied populations was calculated at the level of 0.26 and it was lower than the expected heterozygosity at 0.28. For populations, the level of heterozygosities were as follow: Strzałowo 0.35, Bolesławiec and Białowieża 0.25, Józefów 0.27, Świnoujście 0.23, Woziwoda 0.26. In particular loci level of heterozygosity was different, as the most heterozygous Mdh-C locus was estimated, while minimum Got-C. Significant differences in allele frequency of Hardy-Weinberg deviation equilibrium were found in 10 cases: Strzałowo (Got-C), Bolesławiec (Got-B), Białowieża (Got-B, Gdh), Józefów (Mdh-C), Międzyzdroje (Got-B, Got-C, Mdh-C), Woziwoda (Got-C, Gdh). All studied populations had a lower effective number of alleles per locus (Ne) comparing to the observed number of alleles (Na). Wright’s fixation indices were negative for populations: Strzałowo (-0.09), Józefów (-0.03), Międzyzdroje (-0.01) and positive for: Białowieża (0.12), Woziwoda and Bolesławiec (0.07). The extremely high Wright’s fixation index (0.12) was observed for Białowieża population.

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