Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea

A novel marine actinomycete, designated LHW63021T, was isolated from a marine sponge, genus Craniella, collected in the South China Sea. A polyphasic approach was applied to characterize the taxonomic position of this strain. The strain was found to have scarce aerial mycelia that differentiated into spore chains. The cell-wall hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid. Glucose, galactose, mannose and madurose were found in the whole-cell hydrolysates. The dominant polar lipids were phosphatidylinositol and diphosphatidylglycerol. MK-9(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were iso-C16 : 0, iso-C18 : 0, 10-methyl C17 : 0 and C18 : 1 ω9c. The DNA G+C content based on the draft genome sequence was 72.0 mol%. 16S rRNA gene sequence analysis indicated that strain LHW63021T was a member of the genus Actinomadura and had the highest similarity to Actinomadura echinospora DSM 43163T (97.3 %). Phylogenetic trees supported their close relationship. The average nucleotide identity and digital DNA–DNA hybridization values between the whole genome sequences of strain LHW63021T and A. echinospora DSM 43163T were 79.13 and 23.20 %, respectively. The evidence from the polyphasic study shows that strain LHW63021T represents a novel species of the genus Actinomadura , for which the name Actinomadura craniellae sp. nov. is proposed. The type strain is LHW63021T (=DSM 106125T=CCTCC AA 2018015T).

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Antioxidant Alkaloid from the South China Sea Marine Sponge Iotrochota sp.

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-9-1003 ◽

2008 ◽

Vol 63 (9-10) ◽

pp. 636-638 ◽

Cited By ~ 11

Author(s):

Yonghong Liu ◽

Hong Ji ◽

Junde Dong ◽

Si Zhang ◽

Kyung Jin Lee ◽

...

Keyword(s):

Antioxidant Activity ◽

South China Sea ◽

South China ◽

Marine Sponge ◽

The South China Sea ◽

Dpph Assay ◽

The South ◽

China Sea ◽

Nmr Data

Purpurone was isolated from the sponge Iotrochota sp. by bioactivity-guided fractionation. The compound showed antioxidant activity using the DPPH assay. The structure was established on the basis of NMR data and comparison with data reported.

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Dibromotyrosine and Histamine Derivatives from the Tropical Marine Sponge Aplysina sp

Natural Product Communications ◽

10.1177/1934578x1000500307 ◽

2010 ◽

Vol 5 (3) ◽

pp. 1934578X1000500 ◽

Cited By ~ 2

Author(s):

Elena A. Santalova ◽

Vladimir A. Denisenko ◽

Valentin A. Stonik

Keyword(s):

South China Sea ◽

Chemical Defense ◽

South China ◽

Marine Sponge ◽

The South China Sea ◽

The South ◽

China Sea ◽

New Compounds ◽

C Nmr

Two new compounds, 3-amino-7,8-dihydroimidazo-[1,5-c]-pyrimidin-5(6H)-one (1) and ethyl 3-(2-amino-1H-imidazol-4-yl)propylcarbamate (2), along with the previously known 7,8-dihydroimidazo-[1,5-c]-pyrimidin-5(6H)-one (3), aeroplysinin-1 (4), dibromoverongiaquinol (5), bisoxazolidinone derivative (6), aerophobins-1 (7) and −2 (8), purealidins J (9) and L, have been isolated from Aplysina sp. from the South China Sea. The structures were elucidated on the basis of 1H, 13C NMR, MS and IR analyses. The histamine-derived alkaloids 1-3 may be unknown bioconversion products of purealidin J (9), aerophobin-2 (8) and aerophobin-1 (7), respectively, when 7-9 are cleaved at C-8–C-9 in reactions of activated chemical defense in Aplysina sponge.

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