Genetic variation in mitochondrial genes of the tick Haemaphysalis flava collected from wild hedgehogs in China

Abstract The Apaporis caiman (Caiman crocodilus apaporiensis) has been of particular interest due to its highly differentiated morphology. However, no molecular research has been done to clarify its taxonomy. We characterized the genetic variation within C. crocodilus by assessing the evolutionary trajectory of Apaporis caiman populations using mitochondrial molecular markers. We collected ten Apaporis caiman samples from the middle basin of the Apaporis River, Colombia, sequenced two mitochondrial genes [cytochrome oxidase I (COI) and cytochrome B (CytB)], and analysed them together with all available sequences from homologous gene fragments at GenBank for the species. Phylogenetic reconstructions revealed three main clades clearly differentiated across the C. crocodilus complex. These clades matched genetically and geographically with three of the four subspecies currently recognized (C. c. chiapasius, C. c. fuscus and C. c. crocodilus). However, we found low to almost non-existent genetic differentiation between C. c. crocodilus and the until-now morphologically recognized C. c. apaporiensis, suggesting that the latter is part of the genetic spectrum present within C. c. crocodilus. We reject the hypothesis of an expected elevated level of genetic variation due to isolation (supported by morphological differentiation) and support the idea of Apaporis caiman populations as a C. crocodilus ecomorph.

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Analysis of Genetic Variation in Ribosomal DNA Internal Transcribed Spacer and the NADH Dehydrogenase Subunit 4 Mitochondrial Genes of the Onchocerciasis VectorSimulium ochraceum

Journal of Medical Entomology ◽

10.1093/jmedent/43.4.701 ◽

2006 ◽

Vol 43 (4) ◽

pp. 701-706 ◽

Cited By ~ 8

Author(s):

Mario A. Rodríguez-pérez ◽

Claudia A. Núñez-gonzález ◽

Cristian Lizarazo-ortega ◽

Alejandro Sánchez-varela ◽

Michael C. Wooten ◽

...

Keyword(s):

Genetic Variation ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Nadh Dehydrogenase ◽

Mitochondrial Genes ◽

Nadh Dehydrogenase Subunit

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Genetic Variation and Origin of Parthenogenesis in theAspidoscelis cozumelaComplex: Evidence from Mitochondrial Genes

ZOOLOGICAL SCIENCE ◽

10.2108/zsj.31.14 ◽

2014 ◽

Vol 31 (1) ◽

pp. 14-19 ◽

Cited By ~ 14

Author(s):

Norma L. Manríquez-Morán ◽

Fausto R. Méndez-de la Cruz ◽

Robert W. Murphy

Keyword(s):

Genetic Variation ◽

Mitochondrial Genes

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A Great Difference Between Sedentary Subspecies of Laniarius atroflavus Shelley, 1887 Suggests on the Base of Molecular Data that Laniarius atroflavus and Laniarius craterum are Two Separate Species

The Open Ornithology Journal ◽

10.2174/1874453201407010030 ◽

2014 ◽

Vol 7 (1) ◽

pp. 30-39

Author(s):

Billy Nguembock

Keyword(s):

Genetic Variation ◽

West Africa ◽

Size Variation ◽

Molecular Data ◽

Genetic Distances ◽

Mitochondrial Genes ◽

Secondary Contact ◽

Separate Species ◽

Quaternary Period ◽

Molecular Characters

Laniarius atroflavus is a sedentary member of the bush-shrike radiation (family Malaconotidae) and in our first paper, it appeared with strong divergence between its two subspecies. To confirm this divergence and leaning partially on our first obtained results, we investigated a genetic variation of the individuals of Laniarius atroflavus atroflavus (West Africa) and Laniarius atroflavus craterum (West Africa). For the genetic variation, we use two mitochondrial genes (ATPase6 and ND2) to calculate their genetic distances within the Laniarius ingroup and to explore their mutational differentiation. With our ATPase6 and ND2, a genetic distance of 1.66% and 2.14% has been respectively estimated between individuals of Laniarius atroflavus atroflavus (Cameroon Mountain) and Laniarius atroflavus craterum (Manenguba Mountain) whereas it was, for the same markers, of 0% and 0.57% respectively between specimens of Laniarius atroflavus craterum only caught in diverse parts of the Manenguba Mountain. For the mutational differentiation, a total of 34 different molecular characters have been observed with the two markers investigated between these two subspecies. Leaning on some dating results, it appears that Laniarius atroflavus atroflavus diverged from Laniarius atroflavus craterum during the Quaternary period and these dates correspond remarkably with those suggested for several polyphased volcanic activities noted in the Cameroon Volcanic Line. For these separate sedentary birds which are marked by their clinal size variation, measures of their wings as well as the variation of the color of their breast and belly, a possible secondary contact will certainly lead to reinforcement. Thus, we suggest resurrecting names Laniarius atroflavus Shelley, 1887 and Laniarius craterum Bates, 1926 for individuals of the populations hitherto referred as Laniarius atroflavus atroflavus and Laniarius atroflavus craterum respectively.

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Correction to: First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

Parasites & Vectors ◽

10.1186/s13071-019-3807-y ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

John A. Ohiolei ◽

Joshua Luka ◽

Guo-Qiang Zhu ◽

Hong-Bin Yan ◽

Li Li ◽

...

Keyword(s):

Genetic Variation ◽

Mitochondrial Genes ◽

Taenia Hydatigena ◽

Sheep And Goats ◽

Molecular Description

Following publication of the original article [1], the have authors flagged that the information in the legend of Fig. 1 is detailed in the wrong order.

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Genetic Variation of the Long-Legged FliesPhacaspis mitisComplex (Diptera: Dolichopodidae) in Peninsular Thailand Inferred From Three Mitochondrial Genes

Journal of Insect Science ◽

10.1093/jisesa/iey024 ◽

2018 ◽

Vol 18 (6) ◽

Cited By ~ 1

Author(s):

Natcha Kaewkrajang ◽

Patrick Grootaert ◽

Singtoe Boonrotpong

Keyword(s):

Genetic Variation ◽

Mitochondrial Genes

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First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

Parasites & Vectors ◽

10.1186/s13071-019-3780-5 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 4

Author(s):

John A. Ohiolei ◽

Joshua Luka ◽

Guo-Qiang Zhu ◽

Hong-Bin Yan ◽

Li Li ◽

...

Keyword(s):

Mitochondrial Dna ◽

Genetic Variation ◽

Dna Markers ◽

Nadh Dehydrogenase ◽

Mitochondrial Genes ◽

Full Length ◽

Nadh Dehydrogenase Subunit ◽

Taenia Hydatigena ◽

Sheep And Goats ◽

Geographical Regions

Abstract Background Cysticercosis caused by the metacestode larval stage of Taenia hydatigena is a disease of veterinary and economic importance. A considerable level of genetic variation among isolates of different intermediate hosts and locations has been documented. Generally, data on the genetic population structure of T. hydatigena is scanty and lacking in Nigeria. Meanwhile, similar findings in other cestodes like Echinococcus spp. have been found to be of epidemiological importance. Our aim, therefore, was to characterize and compare the genetic diversity of T. hydatigena population in Nigeria based on three mitochondrial DNA markers as well as to assess the phylogenetic relationship with populations from other geographical regions. Methods In the present study, we described the genetic variation and diversity of T. hydatigena isolates from Nigerian sheep and goats using three full-length mitochondrial genes: the cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunit 1 (nad1), and NADH dehydrogenase subunit 5 (nad5). Results The median-joining network of concatenated cox1-nad1-nad5 sequences indicated that T. hydatigena metacestodes of sheep origin were genetically distinct from those obtained in goats and this was supported by high FST values of nad1, cox1, and concatenated cox1-nad1-nad5 sequences. Genetic variation was also found to be higher in isolates from goats than from sheep. Conclusions To the best of our knowledge, the present study described the genetic variation of T. hydatigena population for the first time in Nigeria using full-length mitochondrial genes and suggests the existence of host-specific variants. The population indices of the different DNA markers suggest that analysis of long mitochondrial DNA fragments may provide more information on the molecular ecology of T. hydatigena. We recommend that future studies employ long mitochondrial DNA sequence in order to provide reliable data that would explain the extent of genetic variation in different hosts/locations and the biological and epidemiological significance.

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