scholarly journals Correction to: First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
John A. Ohiolei ◽  
Joshua Luka ◽  
Guo-Qiang Zhu ◽  
Hong-Bin Yan ◽  
Li Li ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Mitochondrial Genes ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Molecular Description

Following publication of the original article [1], the have authors flagged that the information in the legend of Fig. 1 is detailed in the wrong order.

scholarly journals First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
John A. Ohiolei ◽  
Joshua Luka ◽  
Guo-Qiang Zhu ◽  
Hong-Bin Yan ◽  
Li Li ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Markers ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Genes ◽  
Full Length ◽  
Nadh Dehydrogenase Subunit ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Geographical Regions

Abstract Background Cysticercosis caused by the metacestode larval stage of Taenia hydatigena is a disease of veterinary and economic importance. A considerable level of genetic variation among isolates of different intermediate hosts and locations has been documented. Generally, data on the genetic population structure of T. hydatigena is scanty and lacking in Nigeria. Meanwhile, similar findings in other cestodes like Echinococcus spp. have been found to be of epidemiological importance. Our aim, therefore, was to characterize and compare the genetic diversity of T. hydatigena population in Nigeria based on three mitochondrial DNA markers as well as to assess the phylogenetic relationship with populations from other geographical regions. Methods In the present study, we described the genetic variation and diversity of T. hydatigena isolates from Nigerian sheep and goats using three full-length mitochondrial genes: the cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunit 1 (nad1), and NADH dehydrogenase subunit 5 (nad5). Results The median-joining network of concatenated cox1-nad1-nad5 sequences indicated that T. hydatigena metacestodes of sheep origin were genetically distinct from those obtained in goats and this was supported by high FST values of nad1, cox1, and concatenated cox1-nad1-nad5 sequences. Genetic variation was also found to be higher in isolates from goats than from sheep. Conclusions To the best of our knowledge, the present study described the genetic variation of T. hydatigena population for the first time in Nigeria using full-length mitochondrial genes and suggests the existence of host-specific variants. The population indices of the different DNA markers suggest that analysis of long mitochondrial DNA fragments may provide more information on the molecular ecology of T. hydatigena. We recommend that future studies employ long mitochondrial DNA sequence in order to provide reliable data that would explain the extent of genetic variation in different hosts/locations and the biological and epidemiological significance.

scholarly journals Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan

2020 ◽  
Vol 58 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Rosline James Muku ◽  
Hong-Bin Yan ◽  
John Asekhaen Ohiolei ◽  
Abubakar Ahmed Saaid ◽  
Sara Ahmed ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Larval Stage ◽  
Nadh Dehydrogenase ◽  
Diversity Indices ◽  
Domestic Animals ◽  
Mitochondrial Genes ◽  
Intermediate Hosts ◽  
Nadh Dehydrogenase Subunit ◽  
Taenia Hydatigena ◽  
Low Diversity

The cestode <i>Taenia hydatigena</i> uses canids, primarily dogs, as definitive hosts, while the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep, and pigs. Cysticercosis due to <i>T. hydatigena</i> has large veterinary and economic drawbacks. Like other taeniids, e.g., <i>Echinococcus</i>, intraspecific variation is found among the members of the genus Taenia. In Africa, few studies are available on the epidemiology and distribution of <i>T. hydatigena</i>, and even fewer studies are available on its genetic variation. In this study, we molecularly identified 11 cysticerci from sheep in Sudan and demonstrated the genetic variation based on the NADH dehydrogenase subunit 1 (<i>nad</i>1) and cytochrome c oxidase subunit 1 (<i>cox</i>1) mitochondrial genes. The isolates were correctly identified as <i>T. hydatigena</i> with more than 99% similarity to those in the GenBank database. Low diversity indices and insignificant neutrality indices were observed, with 3 and 2 haplotypes for the <i>nad</i>1 and <i>cox</i>1 genes, respectively. The results suggest the presence of unique <i>T. hydatigena</i> haplotypes in Sudan, as haplotypes with 100% similarity were not found in the GenBank database. With few available studies on the genetic variation of <i>T. hydatigena</i> in Africa, this report represents the first insights into the genetic variation of <i>T. hydatigena</i> in Sudan and constitutes useful data.

The Conglutinating Complement Absorption Test for the Sero-diagnosis of Cysticercus tenuicollis Infection in Sheep and Goats

1973 ◽  
Vol 47 (2) ◽  
pp. 191-197 ◽  
Author(s):  
T. L. Varma ◽  
S. B. Kulshrespha ◽  
B. V. Rao ◽  
S. Kumar
Keyword(s):  
Antibody Response ◽  
Infected Sheep ◽  
Absorption Test ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Cysticercus Tenuicollis

The conglutinating complement absorption test (CCAT) has been used to detect Cysticercus tenuicollis (Taenia hydatigena cysticercus) infection in sheep and goats. Sera from naturally infected sheep and goats showing a titre above 1:40 were considered positive for cysticercosis. Experimentally infected lambs gave a higher antibody response than sheep and goats.

scholarly journals Unveiling the mystery: assessing the evolutionary trajectory of the Apaporis caiman population (Caiman crocodilus apaporiensis, Medem 1955) via mitochondrial molecular makers

2020 ◽  
Vol 131 (1) ◽  
pp. 163-171
Author(s):  
Sergio A Balaguera-Reina ◽  
Mario Vargas-Ramírez ◽  
Nicté Ordóñez-Garza ◽  
Felipe Hernández-González ◽  
Llewellyn D Densmore
Keyword(s):  
Genetic Variation ◽  
Molecular Markers ◽  
Elevated Level ◽  
Morphological Differentiation ◽  
Mitochondrial Genes ◽  
Homologous Gene ◽  
Evolutionary Trajectory ◽  
Caiman Crocodilus ◽  
Phylogenetic Reconstructions ◽  
Molecular Makers

Abstract The Apaporis caiman (Caiman crocodilus apaporiensis) has been of particular interest due to its highly differentiated morphology. However, no molecular research has been done to clarify its taxonomy. We characterized the genetic variation within C. crocodilus by assessing the evolutionary trajectory of Apaporis caiman populations using mitochondrial molecular markers. We collected ten Apaporis caiman samples from the middle basin of the Apaporis River, Colombia, sequenced two mitochondrial genes [cytochrome oxidase I (COI) and cytochrome B (CytB)], and analysed them together with all available sequences from homologous gene fragments at GenBank for the species. Phylogenetic reconstructions revealed three main clades clearly differentiated across the C. crocodilus complex. These clades matched genetically and geographically with three of the four subspecies currently recognized (C. c. chiapasius, C. c. fuscus and C. c. crocodilus). However, we found low to almost non-existent genetic differentiation between C. c. crocodilus and the until-now morphologically recognized C. c. apaporiensis, suggesting that the latter is part of the genetic spectrum present within C. c. crocodilus. We reject the hypothesis of an expected elevated level of genetic variation due to isolation (supported by morphological differentiation) and support the idea of Apaporis caiman populations as a C. crocodilus ecomorph.

Analysis of Genetic Variation in Ribosomal DNA Internal Transcribed Spacer and the NADH Dehydrogenase Subunit 4 Mitochondrial Genes of the Onchocerciasis VectorSimulium ochraceum

2006 ◽  
Vol 43 (4) ◽  
pp. 701-706 ◽  
Author(s):  
Mario A. Rodríguez-pérez ◽  
Claudia A. Núñez-gonzález ◽  
Cristian Lizarazo-ortega ◽  
Alejandro Sánchez-varela ◽  
Michael C. Wooten ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Genes ◽  
Nadh Dehydrogenase Subunit

Genetic variation in mitochondrial genes of the tick Haemaphysalis flava collected from wild hedgehogs in China

2017 ◽  
Vol 71 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Zhong-Bo Li ◽  
Tian-Yin Cheng ◽  
Xing-Li Xu ◽  
Lu-Lin Song ◽  
Guo-Hua Liu
Keyword(s):  
Genetic Variation ◽  
Mitochondrial Genes ◽  
Haemaphysalis Flava

Genetic Variation and Origin of Parthenogenesis in theAspidoscelis cozumelaComplex: Evidence from Mitochondrial Genes

2014 ◽  
Vol 31 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Norma L. Manríquez-Morán ◽  
Fausto R. Méndez-de la Cruz ◽  
Robert W. Murphy
Keyword(s):  
Genetic Variation ◽  
Mitochondrial Genes

scholarly journals Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq

2018 ◽  
pp. 177-182
Author(s):  
Abdullah Ahmed Hama ◽  
Rostam Hama Zorab ◽  
Fatima Mohammed Ali ◽  
Awaz M. Salih ◽  
Amer Abdullah Hassan
Keyword(s):  
Hydatid Cyst ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Prevalence Rate ◽  
Accession Number ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Cysticercus Tenuicollis ◽  
Nucleotide Sequence Alignment

Cysticercus tenuicollis (C. tenuicollis) is the larval stage infection of Taenia hydatigena, a common tapeworm of dogs and other Canidae, which has a wide range of intermediate hosts including sheep, goat, cattle, deer, camel, horse, human and other wild ruminants, the disease spreads through a contaminated water, soil and food with feces of infected dogs or other carnivores, T. hydatigena lives in the intestinal of the definitive host (carnivores) and excretes a huge number of eggs with feces daily. The present study conducted to determine the prevalence rate and molecular characterization of C. tenuicollis among sheep and goats in Sulaimani province. A total of 14088 slaughtered animals were inspected postmortem from the new Sulaimani abattoir comprise, which involves 13395 sheep and 693 goats. The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study,  The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. The prevalence rate of C. tenuicollis was 2.63% in sheep and 2.58% in goats. This result shows no significant differences of C. tenuicollis between sheep and goats (p>0.05). The nucleotide sequence alignment of cytochrome oxidase subunit 1 (CO 1) gene revealed that the amplified DNA fragment is belong to Taenia hydatigena and Echinococcus granulosus and the nucleotide sequences of T. hydatigena deposited in GenBank under accession number (MH638348). This finding concludes that the amplification of mt-CO1 gene cannot be depended on discriminate hydatid cyst and C. tenuicollis while the partial DNA sequences of mt-CO1 gene are significantly valuable to differentiate C. tenuicollis from hydatid cyst, which is completely different in the pathology and control.

scholarly journals A Great Difference Between Sedentary Subspecies of Laniarius atroflavus Shelley, 1887 Suggests on the Base of Molecular Data that Laniarius atroflavus and Laniarius craterum are Two Separate Species

2014 ◽  
Vol 7 (1) ◽  
pp. 30-39
Author(s):  
Billy Nguembock
Keyword(s):  
Genetic Variation ◽  
West Africa ◽  
Size Variation ◽  
Molecular Data ◽  
Genetic Distances ◽  
Mitochondrial Genes ◽  
Secondary Contact ◽  
Separate Species ◽  
Quaternary Period ◽  
Molecular Characters

Laniarius atroflavus is a sedentary member of the bush-shrike radiation (family Malaconotidae) and in our first paper, it appeared with strong divergence between its two subspecies. To confirm this divergence and leaning partially on our first obtained results, we investigated a genetic variation of the individuals of Laniarius atroflavus atroflavus (West Africa) and Laniarius atroflavus craterum (West Africa). For the genetic variation, we use two mitochondrial genes (ATPase6 and ND2) to calculate their genetic distances within the Laniarius ingroup and to explore their mutational differentiation. With our ATPase6 and ND2, a genetic distance of 1.66% and 2.14% has been respectively estimated between individuals of Laniarius atroflavus atroflavus (Cameroon Mountain) and Laniarius atroflavus craterum (Manenguba Mountain) whereas it was, for the same markers, of 0% and 0.57% respectively between specimens of Laniarius atroflavus craterum only caught in diverse parts of the Manenguba Mountain. For the mutational differentiation, a total of 34 different molecular characters have been observed with the two markers investigated between these two subspecies. Leaning on some dating results, it appears that Laniarius atroflavus atroflavus diverged from Laniarius atroflavus craterum during the Quaternary period and these dates correspond remarkably with those suggested for several polyphased volcanic activities noted in the Cameroon Volcanic Line. For these separate sedentary birds which are marked by their clinal size variation, measures of their wings as well as the variation of the color of their breast and belly, a possible secondary contact will certainly lead to reinforcement. Thus, we suggest resurrecting names Laniarius atroflavus Shelley, 1887 and Laniarius craterum Bates, 1926 for individuals of the populations hitherto referred as Laniarius atroflavus atroflavus and Laniarius atroflavus craterum respectively.

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