Effect of oxidation–reduction potential on performance of European sea bass (Dicentrarchus labrax) in recirculating aquaculture systems

European sea bass and Ulva sp. were co-cultured in different tanks of an indoor Recirculating Aquaculture System (Ulva-RAS) with bacterial biofilter, in an effort to optimize the efficiency of the system and to further decrease the waste effluent. A system with similar culture conditions, without Ulva, was used as a control-RAS to elucidate integration effects on growth performance and chemical composition of sea bass. The role of Ulva on N and P concentrations, gas (O2, CO2) and pH in water was also investigated. Fish were fed a diet of fish oil replacement (55%) with a mixture of rapeseed oil and palm oil (1:1). Our data showed that Ulva could uptake N and P nutrients, but could also enrich sea water with phosphates. Sea bass reared in Ulva-RAS exhibited isometric growth, while fish in control-RAS showed a positive allometric growth and an increased variance of body weight and length. In addition, sea bass in Ulva-RAS demonstrated significantly higher levels of condition factor (K), feed intake, protein, lipid, P, EPA and DHA content (% wet weight of total body) and lipid productive value, compared to fish in control-RAS. Ulva, after bi-weekly culture, showed increased protein content (60%) compared to wild seaweed collected nearshore. Cultivated Ulva obtained dark green color, doubled chlorophyll concentrations, and exhibited lower levels of saturated and higher levels of certain monounsaturated and n-3 polyunsaturated fatty acids, indicating increased photosynthetic activity. Present results revealed the beneficial effects of Ulva on sea bass growth and quality, which led to an improved response to the nutritional stress imposed by the fish oil replacement with vegetable oils, thus contributing to a sustainable aquaculture. Moreover, it was concluded that Ulva could improve water quality by increasing pH and O2, reducing CO2 and contribute to bioremediation of ammonia and nitrates from water in integrated aquaculture.

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High levels of seminal oxidation reduction potential (ORP) in infertile men with clinical varicocele

10.26226/morressier.5912d9e7d462b80292386967 ◽

2017 ◽

Author(s):

Ramadan Saleh

Keyword(s):

Reduction Potential ◽

Infertile Men ◽

Oxidation Reduction ◽

Oxidation Reduction Potential

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Vaccination of European sea bass Dicentrarchus labrax with avirulent Mycobacterium marinum (iipA::kan mutant)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2019.04.057 ◽

2019 ◽

Vol 90 ◽

pp. 317-327 ◽

Cited By ~ 1

Author(s):

Shay Ravid-Peretz ◽

Angelo Colorni ◽

Galit Sharon ◽

Michal Ucko

Keyword(s):

Dicentrarchus Labrax ◽

Sea Bass ◽

Mycobacterium Marinum ◽

European Sea Bass

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On the Elucidation of the pH Dependence of the Oxidation-Reduction Potential of Cytochrome c at Alkaline pH

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)99768-1 ◽

1966 ◽

Vol 241 (18) ◽

pp. 4180-4185

Author(s):

Karl G. Brandt ◽

Paul C. Parks ◽

Georg H. Czerlinski ◽

George P. Hess

Keyword(s):

Cytochrome C ◽

Reduction Potential ◽

Ph Dependence ◽

Alkaline Ph ◽

Oxidation Reduction ◽

Oxidation Reduction Potential

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In situ measurements of oxidation–reduction potential and hydrogen peroxide concentration as tools for revealing LPMO inactivation during enzymatic saccharification of cellulose

Biotechnology for Biofuels ◽

10.1186/s13068-021-01894-1 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Adnan Kadić ◽

Anikó Várnai ◽

Vincent G. H. Eijsink ◽

Svein Jarle Horn ◽

Gunnar Lidén

Keyword(s):

Reduction Potential ◽

Enzymatic Saccharification ◽

The State ◽

Enzyme Inactivation ◽

Ex Situ ◽

Process Economics ◽

Complete Inactivation ◽

Oxidation Reduction ◽

Oxidation Reduction Potential

Abstract Background Biochemical conversion of lignocellulosic biomass to simple sugars at commercial scale is hampered by the high cost of saccharifying enzymes. Lytic polysaccharide monooxygenases (LPMOs) may hold the key to overcome economic barriers. Recent studies have shown that controlled activation of LPMOs by a continuous H2O2 supply can boost saccharification yields, while overdosing H2O2 may lead to enzyme inactivation and reduce overall sugar yields. While following LPMO action by ex situ analysis of LPMO products confirms enzyme inactivation, currently no preventive measures are available to intervene before complete inactivation. Results Here, we carried out enzymatic saccharification of the model cellulose Avicel with an LPMO-containing enzyme preparation (Cellic CTec3) and H2O2 feed at 1 L bioreactor scale and followed the oxidation–reduction potential and H2O2 concentration in situ with corresponding electrode probes. The rate of oxidation of the reductant as well as the estimation of the amount of H2O2 consumed by LPMOs indicate that, in addition to oxidative depolymerization of cellulose, LPMOs consume H2O2 in a futile non-catalytic cycle, and that inactivation of LPMOs happens gradually and starts long before the accumulation of LPMO-generated oxidative products comes to a halt. Conclusion Our results indicate that, in this model system, the collapse of the LPMO-catalyzed reaction may be predicted by the rate of oxidation of the reductant, the accumulation of H2O2 in the reactor or, indirectly, by a clear increase in the oxidation–reduction potential. Being able to monitor the state of the LPMO activity in situ may help maximizing the benefit of LPMO action during saccharification. Overcoming enzyme inactivation could allow improving overall saccharification yields beyond the state of the art while lowering LPMO and, potentially, cellulase loads, both of which would have beneficial consequences on process economics.

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The relation of pH and oxidation-reduction potential to the association state of the ferredoxin . ferredoxin:NADP+ reductase complex.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)43341-8 ◽

1981 ◽

Vol 256 (15) ◽

pp. 7756-7763 ◽

Cited By ~ 5

Author(s):

C.J. Batie ◽

H. Kamin

Keyword(s):

Reduction Potential ◽

Oxidation Reduction ◽

Oxidation Reduction Potential ◽

Association State

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The Negative Impact of Varicocele on Basic Semen Parameters, Sperm Nuclear DNA Dispersion and Oxidation-Reduction Potential in Semen

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18115977 ◽

2021 ◽

Vol 18 (11) ◽

pp. 5977

Author(s):

Kamil Gill ◽

Michal Kups ◽

Patryk Harasny ◽

Tomasz Machalowski ◽

Marta Grabowska ◽

...

Keyword(s):

Reduction Potential ◽

Nuclear Dna ◽

Study Groups ◽

Semen Parameters ◽

Sperm Dna Fragmentation ◽

Control Groups ◽

Infertile Men ◽

Oxidation Reduction ◽

Oxidation Reduction Potential ◽

Sperm Dna

Since varicocele is so common in infertile men, this study intends to analyse the relationships between varicocele and conventional semen characteristics, sperm nuclear DNA dispersion and oxidation-reduction potential (ORP) in semen. Varicocele-positive and varicocele-negative infertile men (study groups) showed significantly lower standard sperm parameters and higher sperm DNA fragmentation (SDF) and ORP in semen than healthy volunteers and subjects with proven fertility (control groups). A lower proportion of low SDF levels (0–15% SDF) and higher incidence of high SDF levels (>30% SDF), as well as a higher prevalence of high ORP values (>1.37 mV/106 sperm/mL), were found in the study groups vs. the control groups. Moreover, infertile men had significantly lower odds ratios (ORs) for low SDF levels and significantly higher ORs for high SDF levels and high ORP. SDF and ORP were negatively correlated with sperm number, morphology, motility and vitality. Furthermore, a significant positive correlation was found between SDF and ORP. The obtained results suggest that disorders of spermatogenesis may occur in varicocele-related infertility. These abnormalities are manifested not only by reduced standard semen parameters but also by decreased sperm DNA integrity and simultaneously increased oxidative stress in semen.

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