Effect of Heterologous Expression of Chemotaxis Proteins from Genus Thermotoga on the Growth Kinetics of Escherichia coli Cells

2019 ◽  
Vol 167 (3) ◽  
pp. 375-379 ◽  
Author(s):  
D. V. Grishin ◽  
V. A. Samoilenko ◽  
Yu. A. Gladilina ◽  
D. D. Zhdanov ◽  
M. V. Pokrovskaya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Growth Kinetics ◽  
Escherichia Coli Cells ◽  
Chemotaxis Proteins ◽  
Kinetics Of

scholarly journals Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells

1991 ◽  
Vol 276 (3) ◽  
pp. 637-641 ◽  
Author(s):  
F F Craig ◽  
A C Simmonds ◽  
D Watmore ◽  
F McCapra ◽  
M R H White
Keyword(s):  
Escherichia Coli ◽  
Mammalian Cells ◽  
Firefly Luciferase ◽  
Luciferase Expression ◽  
Intact Cells ◽  
Cos Cells ◽  
Escherichia Coli Cells ◽  
Luminescent Signal ◽  
The Difference ◽  
Kinetics Of

Five esters of luciferin were synthesized and compared with native luciferin as substrates for firefly luciferase expressed in live intact mammalian cells. The esters themselves were not substrates for purified luciferase, but four were substrates for a purified esterase and all appeared to be hydrolysed to luciferin within mammalian cells. At a substrate concentration of 0.01 mM, the peak luminescence from the cos cells expressing luciferase was up to 6-fold greater with the esters than with unmodified luciferin. At 0.1 mM, the difference between luciferin and the esters was decreased. The kinetics of the luminescent signal with the different luciferin esters varied significantly, indicating possible differences in the rates of uptake, breakdown and enzyme inhibition. The esters did not support luminescence from Escherichia coli cells expressing firefly luciferase, suggesting a lack of appropriate esterase activity in this particular strain. The esters could be useful for the assay of luciferase expression in intact mammalian cells when luciferin levels are limiting, for example in tissues, and in plants. Alternative luciferin derivatives may allow further improvements in sensitivity.

Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells

1990 ◽  
Vol 12 (11) ◽  
pp. 854-859 ◽  
Author(s):  
George P. Philippidis ◽  
Janet L. Schottel ◽  
Wei-Shou Hu
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli Cells ◽  
Kinetics Of
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