Expression and Purification of Biologically Active Rat Bone Morphogenetic Protein-4 produced as Inclusion Bodies in Recombinant Escherichia coli

2005 ◽  
Vol 27 (20) ◽  
pp. 1559-1564 ◽  
Author(s):  
Burkhard Klösch ◽  
Walter Fürst ◽  
Rudolf Kneidinger ◽  
Monika Schuller ◽  
Barbara Rupp ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bone Morphogenetic Protein ◽  
Inclusion Bodies ◽  
Biologically Active ◽  
Recombinant Escherichia Coli ◽  
Bone Morphogenetic Protein 4 ◽  
Expression And Purification ◽  
Morphogenetic Protein ◽  
Rat Bone

scholarly journals Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies

2018 ◽  
Vol 18 ◽  
pp. e00249
Author(s):  
Gesa-Maria Gieseler ◽  
Kimia Ekramzadeh ◽  
Volker Nölle ◽  
Svitlana Malysheva ◽  
Henning Kempf ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Inclusion Bodies ◽  
Human Bone ◽  
Biologically Active ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein

Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli

1999 ◽  
Vol 64 (8) ◽  
pp. 1348-1356 ◽  
Author(s):  
Michaela Rumlová-Kliková ◽  
Iva Pichová ◽  
Eric Hunter ◽  
Tomáš Ruml
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Amorphous Material ◽  
Bacterial Expression ◽  
Biologically Active ◽  
Capsid Assembly ◽  
Cultivation Medium ◽  
Viral Particles ◽  
Induction Temperature

It has been generally accepted that inclusion bodies (IBs) formed in Escherichia coli consist of non-biologically active aggregated proteins, which are stabilized by non-productive interactions. We show here that bacterial expression of a retroviral capsid polyprotein results in formation of insoluble IBs that contain fully assembled viral particles connected with amorphous material. The efficiency of IBs formation and capsid assembly was not significantly affected by changes in induction temperature, pH of cultivation medium or the level of expression.

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