Molecular cloning, recombinant expression, and antifungal functional characterization of the lipid transfer protein from Panax ginseng

2016 ◽  
Vol 38 (7) ◽  
pp. 1229-1235 ◽  
Author(s):  
Kexin Cai ◽  
Jiawen Wang ◽  
Min Wang ◽  
Hui Zhang ◽  
Siming Wang ◽  
...  
2018 ◽  
Vol 30 (3) ◽  
pp. 1149-1158
Author(s):  
Nataliya Hrunyk ◽  
Valentina Kovaleva ◽  
Hryhoriy Krynytskyy ◽  
Ivan Gout ◽  
Francisco Amil-Ruiz ◽  
...  

Acta Naturae ◽  
2015 ◽  
Vol 7 (3) ◽  
pp. 65-73 ◽  
Author(s):  
I. V. Bogdanov ◽  
E. I. Finkina ◽  
S. V. Balandin ◽  
D. N. Melnikova ◽  
E. A. Stukacheva ◽  
...  

The recombinant isoforms Lc-LTP1 and Lc-LTP3 of the lentil lipid transfer protein were overexpressed in E. coli cells. It was confirmed that both proteins are stabilized by four disulfide bonds and characterized by a high proportion of the -helical structure. It was found that Lc-LTP1 and Lc-LTP3 possess antimicrobial activity and can bind fatty acids. Both isoforms have the ability to bind specific IgE from sera of patients with food allergies, which recognize similar epitopes of the major peach allergen Pru p 3. Both isoforms were shown to have immunological properties similar to those of other plant allergenic LTPs, but Lc-LTP3 displayed a less pronounced immunoreactivity.


2003 ◽  
Vol 794 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Mariana S. Castro ◽  
Isabel R. Gerhardt ◽  
Stefania Orrù ◽  
Piero Pucci ◽  
Carlos Bloch

2010 ◽  
Vol 17 (3) ◽  
pp. 311-318 ◽  
Author(s):  
Luana Cruz ◽  
Suzanna Ribeiro ◽  
Andre Carvalho ◽  
Ilka Vasconcelos ◽  
Rosana Rodrigues ◽  
...  

Plant Science ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Anne K Kristensen ◽  
Janne Brunstedt ◽  
Klaus K Nielsen ◽  
Peter Roepstorff ◽  
Jorn D Mikkelsen

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