A perfusable microfluidic device with on-chip total internal reflection fluorescence microscopy (TIRFM) for in situ and real-time monitoring of live cells

2012 ◽  
Vol 14 (4) ◽  
pp. 791-797 ◽  
Author(s):  
Ryuji Yokokawa ◽  
Yuko Kitazawa ◽  
Kyohei Terao ◽  
Atsuhito Okonogi ◽  
Isaku Kanno ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Microfluidic Device ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Real Time Monitoring ◽  
On Chip

Visualizing membrane trafficking using total internal reflection fluorescence microscopy

2003 ◽  
Vol 31 (4) ◽  
pp. 819-823 ◽  
Author(s):  
V. Beaumont
Keyword(s):  
Fluorescence Microscopy ◽  
Membrane Trafficking ◽  
Cell Biology ◽  
Total Internal Reflection ◽  
Optical Resolution ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Live Cells ◽  
Excitable Cells ◽  
Cellular Processes

There is a dizzying array of fluorescent probes now commercially available to monitor cellular processes, and advances in molecular biology have highlighted the ease with which proteins can now be labelled with fluorophores without loss of functionality. This has led to an explosion in the popularity of fluorescence microscopy techniques. One such specialized technique, total internal reflection fluorescence microscopy (TIR-FM), is ideally suited to gaining insight into events occurring at, or close to, the plasma membrane of live cells with excellent optical resolution. In the last few years, the application of TIR-FM to membrane trafficking events in both non-excitable and excitable cells has been an area of notable expansion and fruition. This review gives a brief overview of that literature, with emphasis on the study of the regulation of exocytosis and endocytosis in excitable cells using TIR-FM. Finally, recent applications of TIR-FM to the study of cellular processes at the molecular level are discussed briefly, providing promise that the future of TIR-FM in cell biology will only get brighter.

scholarly journals Monitoring the Real-Time Binding of Tropomyosin to Actin using Total Internal Reflection Fluorescence Microscopy

2011 ◽  
Vol 100 (3) ◽  
pp. 302a
Author(s):  
William M. Schmidt ◽  
Paul Leavis ◽  
William Lehman ◽  
Jeffrey Moore
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
The Real
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