Yeasts identification in microfluidic devices using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH)

2017 ◽  
Vol 19 (1) ◽  
Author(s):  
André M. Ferreira ◽  
Daniela Cruz-Moreira ◽  
Laura Cerqueira ◽  
João M. Miranda ◽  
Nuno F. Azevedo
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Peptide Nucleic Acid ◽  
Microfluidic Devices ◽  
Pna Fish

Response surface methodology to optimize peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) in Saccharomyces cerevisiae

LWT ◽  
2017 ◽  
Vol 80 ◽  
pp. 27-31 ◽  
Author(s):  
Rita S. Santos ◽  
Carolina C. Lima ◽  
Daniel Carvalho ◽  
Francisco Meireles ◽  
Nuno Guimarães ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Response Surface Methodology ◽  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Response Surface ◽  
Peptide Nucleic Acid ◽  
Pna Fish

scholarly journals Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures

1999 ◽  
Vol 37 (9) ◽  
pp. 2760-2765 ◽  
Author(s):  
Henrik Stender ◽  
Kaare Lund ◽  
Kenneth H. Petersen ◽  
Ole F. Rasmussen ◽  
Poonpilas Hongmanee ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Peptide Nucleic Acid ◽  
Nontuberculous Mycobacterium ◽  
Diagnostic Specificity ◽  
Valid Test ◽  
Test Result ◽  
Pna Fish

TB PNA FISH is a new fluorescence in situ hybridization (FISH) method using peptide nucleic acid (PNA) probes for differentiation between species of the Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) in acid-fast bacillus-positive (AFB+) cultures is described. The test is based on fluorescein-labelled PNA probes that target the rRNA of MTC or NTM species applied to smears of AFB+ cultures for microscopic examination. Parallel testing with the two probes serves as an internal control for each sample such that a valid test result is based on one positive and one negative reaction. TB PNA FISH was evaluated with 30 AFB+ cultures from Denmark and 42 AFB+ cultures from Thailand. The MTC-specific PNA probe showed diagnostic sensitivities of 84 and 97%, respectively, and a diagnostic specificity of 100% in both studies, whereas the NTM-specific PNA probe showed diagnostic sensitivities of 91 and 64%, respectively, and a diagnostic specificity of 100% in both studies. The low sensitivity of the NTM-specific PNA probe in the Thai study was due to a relatively high prevalence of Mycobacterium fortuitum, which is not identified by the probe. In total, 63 (87%) of the cultures were correctly identified as MTC (n = 46) or NTM (n = 17), whereas the remaining 9 were negative with both probes and thus the results were inconclusive. None of the samples were incorrectly identified as MTC or NTM; thus, the predictive value of a valid test result obtained with TB PNA FISH was 100%.

scholarly journals Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH)

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e14786 ◽  
Author(s):  
Carina Almeida ◽  
Nuno F. Azevedo ◽  
Sílvio Santos ◽  
Charles W. Keevil ◽  
Maria J. Vieira
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Peptide Nucleic Acid ◽  
Pna Fish
Sign in / Sign up

Export Citation Format

Share Document