The cytotoxicity of zinc oxide nanoparticles to 3D brain organoids results from excessive intracellular zinc ions and defective autophagy

Abstract The work presents the method for preparation of zinc oxide nanoparticles with galactose coating. Bare zinc oxide nanoparticles are well-known and popular drug delivers. However, the fact that zinc ions may be released from their structure easily, they may pose a threat to the living organism. Thus the modification of such a product has been performed. The physicochemical properties of the products have been analysed. XRD technique revealed crystallographic structure of the products. Based on ATR-FTIR analysis it was confirmed that galactose has been successfully attached to the zinc oxide nanoparticles. TEM-EDS microscopy was applied in order to assess the shape of nanoparticles and also for the confirmation of galactose on the particles surface. The releasing of zinc ions from the modified products was compared to their releasing from basic, non-modified sample. Also, cytotoxicity and proliferation of obtained products have been analysed with using Chinese hamster ovary cells. It was found out that the proposed technology may lead to obtain stable forms of modified zinc oxide nanoparticles with limited toxicity.

Download Full-text

Disposition and measured toxicity of zinc oxide nanoparticles and zinc ions against keratinocytes in cell culture and viable human epidermis

Nanotoxicology ◽

10.1080/17435390.2019.1692382 ◽

2020 ◽

Vol 14 (2) ◽

pp. 263-274 ◽

Cited By ~ 4

Author(s):

Amy M. Holmes ◽

Lorraine Mackenzie ◽

Michael S. Roberts

Keyword(s):

Cell Culture ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Human Epidermis ◽

Oxide Nanoparticles ◽

Zinc Ions

Download Full-text

Absorption of zinc ions dissolved from zinc oxide nanoparticles in the tobacco callus improves plant productivity

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-019-01636-0 ◽

2019 ◽

Vol 138 (2) ◽

pp. 377-385 ◽

Cited By ~ 3

Author(s):

Shizue Yoshihara ◽

Kasumi Yamamoto ◽

Yoshino Nakajima ◽

Satomi Takeda ◽

Kensuke Kurahashi ◽

...

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Plant Productivity ◽

Oxide Nanoparticles ◽

Tobacco Callus ◽

Zinc Ions

Download Full-text

Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation

Particle and Fibre Toxicology ◽

10.1186/s12989-020-00379-7 ◽

2020 ◽

Vol 17 (1) ◽

Author(s):

Zixuan Liu ◽

Xuying Lv ◽

Lei Xu ◽

Xuting Liu ◽

Xiangyu Zhu ◽

...

Keyword(s):

Cell Death ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Autophagic Cell Death ◽

Oxide Nanoparticles ◽

Zinc Ions ◽

Zno Nps ◽

Toxicological Effects ◽

Major Mode ◽

Free Zinc

Abstract Background With the development of zinc oxide nanoparticles (ZnO NPs) in the field of nanotechnology, their toxicological effects are attracting increasing attention, and the mechanisms for ZnO NPs neurotoxicity remain obscure. In an attempt to address concerns regarding neurotoxicity of ZnO NPs, we explored the relationship between free zinc ions, reactive oxygen species (ROS) and neurotoxic mechanisms in ZnO NPs-exposed PC12 cells. Result This study demonstrated the requirement of free zinc ions shed by ZnO NPs to over generation of intracellular ROS. Next, we identified autophagic cell death was the major mode of cell death induced by ZnO NPs, and autophagosome accumulation resulted from not only induction of autophagy, but also blockade of autophagy flux. We concluded that autophagic cell death, resulting from zinc ions-ROS-c-Jun N-terminal kinase (JNK)-autophagy positive feedback loop and blockade of autophagosomal-lysosomal fusion, played a major role in the neurotoxicity of ZnO NPs. Conclusion Our study contributes to a better understanding of the neurotoxicity of ZnO NPs and might be useful for designing and developing new biosafety nanoparticles in the future.

Download Full-text