scholarly journals Quantitative phenotyping of grey leaf spot disease in maize using real-time PCR

2011 ◽  
Vol 133 (2) ◽  
pp. 461-471 ◽  
Author(s):  
J. Korsman ◽  
B. Meisel ◽  
F. J. Kloppers ◽  
B. G. Crampton ◽  
D. K. Berger
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leaf Spot ◽  
Leaf Spot Disease ◽  
Grey Leaf Spot ◽  
Spot Disease

scholarly journals Identification of Transcripts Involved in Resistance Responses to Leaf Spot Disease Caused by Cercosporidium personatum in Peanut (Arachis hypogaea)

2005 ◽  
Vol 95 (4) ◽  
pp. 381-387 ◽  
Author(s):  
M. Luo ◽  
P. Dang ◽  
M. G. Bausher ◽  
C. C. Holbrook ◽  
R. D. Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
Leaf Spot ◽  
Leaf Spot Disease ◽  
Disease Resistance Gene ◽  
Cercosporidium Personatum ◽  
Spot Disease

Late leaf spot disease caused by Cercosporidium personatum is one of the most destructive foliar diseases of peanut (Arachis hypogaea) worldwide. The objective of this research was to identify resistance genes in response to leaf spot disease using microarray and real-time polymerase chain reaction (PCR). To identify transcripts involved in disease resistance, we studied the gene expression profiles in two peanut genotypes, resistant or susceptible to leaf spot disease, using cDNA microarray containing 384 unigenes selected from two expressed sequenced tag (EST) cDNA libraries challenged by abiotic and biotic stresses. A total of 112 spots representing 56 genes in several functional categories were detected as up-regulated genes (log2 ratio > 1). Seventeen of the top 20 genes, each matching gene with known function in GenBank, were selected for validation of their expression levels using real-time PCR. The two peanut genotypes were also used to study the functional analysis of these genes and the possible link of these genes to the disease resistance trait. Microarray technology and real-time PCR were used for comparison of gene expression. The selected genes identified by microarray analysis were validated by real-time PCR. These genes were more greatly expressed in the resistant genotype as a result of response to the challenge of C. personatum than in the susceptible genotype. Further investigations are needed to characterize each of these genes in disease resistance. Gene probes could then be developed for application in breeding programs for marker-assisted selection.

Cercospora zeina is the causal agent of grey leaf spot disease of maize in southern Africa

2009 ◽  
Vol 124 (4) ◽  
pp. 577-583 ◽  
Author(s):  
Barbara Meisel ◽  
Jeanne Korsman ◽  
Frederik J. Kloppers ◽  
Dave K. Berger
Keyword(s):  
Southern Africa ◽  
Leaf Spot ◽  
Causal Agent ◽  
Leaf Spot Disease ◽  
Grey Leaf Spot ◽  
Spot Disease ◽  
Cercospora Zeina

scholarly journals Characterization of Neopestalotiopsis Species Associated with Mango Grey Leaf Spot Disease in Sinaloa, Mexico

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 788
Author(s):  
Saida S. Gerardo-Lugo ◽  
Juan M. Tovar-Pedraza ◽  
Sajeewa S. N. Maharachchikumbura ◽  
Miguel A. Apodaca-Sánchez ◽  
Kamila C. Correia ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Leaf Spot ◽  
Elongation Factor ◽  
Its Region ◽  
Morphological Characterization ◽  
Leaf Spot Disease ◽  
Grey Leaf Spot ◽  
Spot Disease ◽  
Leaf Spots ◽  
Mango Leaves

Mango is one of the most popular and nutritious fruits in the world and Mexico is the world’s largest exporter. There are many diseases that directly affect fruit yield and quality. During the period 2016–2017, leaves with grey leaf spots were collected from 28 commercial mango orchards distributed in two main production areas in Sinaloa State of Mexico, and 50 Neopestalotiopsis isolates were obtained. Fungal identification of 20 representative isolates was performed using morphological characterization and phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA, part of the translation elongation factor 1-alpha (TEF) and the β-tubulin (TUB) genes. Phylogenetic analysis indicated that the 20 isolates from this study formed four consistent groups, however, overall tree topologies do not consistently provide a stable and sufficient resolution. Therefore, even though morphological and phylogenetic separation is evident, these isolates were not assigned to any new taxa and were tentatively placed into four clades (clades A–D). Pathogenicity tests on detached mango leaves of cv. Kent showed that the 20 isolates that belong to the four Neopestalotiopsis clades from this study and induce lesions on mango leaves. This is the first report of species of Neopestalotiopsis causing mango grey leaf spot disease in Mexico.

scholarly journals Status of grey leaf spot disease in Kenyan maize production ecosystems

2011 ◽  
Vol 18 (4) ◽  
Author(s):  
ZM Kinyua ◽  
JJ Smith ◽  
GN Kibata ◽  
SA Simons ◽  
BC Langat
Keyword(s):  
Leaf Spot ◽  
Leaf Spot Disease ◽  
Maize Production ◽  
Grey Leaf Spot ◽  
Spot Disease

Testing the capability of spectral resolution of the new multispectral sensors on detecting the severity of grey leaf spot disease in maize crop

2017 ◽  
Vol 33 (11) ◽  
pp. 1223-1236 ◽  
Author(s):  
Inos Dhau ◽  
Elhadi Adam ◽  
Onisimo Mutanga ◽  
Kwabena Ayisi ◽  
Elfatih Mohamed Abdel-Rahman ◽  
...  
Keyword(s):  
Spectral Resolution ◽  
Leaf Spot ◽  
Leaf Spot Disease ◽  
Maize Crop ◽  
Grey Leaf Spot ◽  
Spot Disease ◽  
Multispectral Sensors
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