Characterisation of turnip mosaic virus isolates reveals high genetic variability and occurrence of pathotype 1 in Brazil

2021 ◽  
Author(s):  
Leilane Karam Rodrigues ◽  
Alexandre Levi Rodrigues Chaves ◽  
Elliot Watanabe Kitajima ◽  
Renata Faier Calegario ◽  
Katia Regiane Brunelli ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
High Genetic Variability ◽  
Virus Isolates

scholarly journals Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 430 ◽  
Author(s):  
Miroslav Glasa ◽  
Katarína Šoltys ◽  
Lukáš Predajňa ◽  
Nina Sihelská ◽  
Slavomíra Nováková ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Turnip Mosaic Virus ◽  
Biological Assays ◽  
Genetic Complexity ◽  
The World ◽  
History Of ◽  
Virus Isolates

In recent years, the accumulated molecular data of Turnip mosaic virus (TuMV) isolates from various hosts originating from different parts of the world considerably helped to understand the genetic complexity and evolutionary history of the virus. In this work, four complete TuMV genomes (HC9, PK1, MS04, MS15) were characterised from naturally infected cultivated and wild-growing Papaver spp., hosts from which only very scarce data were available previously. Phylogenetic analyses showed the affiliation of Slovak Papaver isolates to the world-B and basal-B groups. The PK1 isolate showed a novel intra-lineage recombination pattern, further confirming the important role of recombination in the shaping of TuMV genetic diversity. Biological assays indicated that the intensity of symptoms in experimentally inoculated oilseed poppy are correlated to TuMV accumulation level in leaves. This is the first report of TuMV in poppy plants in Slovakia.

Molecular Characterization of the 3'-Terminal Region of Turnip mosaic virus Isolates from Eastern China

2007 ◽  
Vol 155 (6) ◽  
pp. 333-341 ◽  
Author(s):  
Y.-P. Tian ◽  
X.-P. Zhu ◽  
J.-L. Liu ◽  
X.-Q. Yu ◽  
J. Du ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Eastern China ◽  
Turnip Mosaic Virus ◽  
Terminal Region ◽  
Virus Isolates

Molecular characterisation of Turnip mosaic virus isolates from Brassicaceae weeds

2008 ◽  
Vol 124 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Shirin Farzadfar ◽  
Yasuhiro Tomitaka ◽  
Mutsumi Ikematsu ◽  
Ali Reza Golnaraghi ◽  
Reza Pourrahim ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Molecular Characterisation ◽  
Virus Isolates

Genetic variability of watermelon mosaic virus isolates infecting cucurbit crops in Italy

2020 ◽  
Vol 165 (4) ◽  
pp. 937-946
Author(s):  
Sabrina Bertin ◽  
Ariana Manglli ◽  
Michael McLeish ◽  
Laura Tomassoli
Keyword(s):  
Genetic Variability ◽  
Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Virus Isolates

scholarly journals Identification of Turnip mosaic virus Isolates Causing Yellow Stripe and Spot on Calla Lily

Plant Disease ◽  
2003 ◽  
Vol 87 (8) ◽  
pp. 901-905 ◽  
Author(s):  
C. C. Chen ◽  
C. H. Chao ◽  
C. C. Chen ◽  
S. D. Yeh ◽  
H. T. Tsai ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Natural Infection ◽  
Sodium Dodecyl ◽  
First Record ◽  
Turnip Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Calla Lily ◽  
Black Magic ◽  
Yellow Stripe ◽  
Virus Isolates

Two virus cultures, RC4 and YC5, were isolated in Taiwan from calla lily (Zantedeschia spp.) cv. Black magic displaying yellow spot and stripe on leaves. Both isolates were mechanically transmitted to various hybrids of Zantedeschia and induced systemic symptoms similar to those observed on diseased Black magic. In addition to Zantedeschia spp., the two virus isolates also infected several cruciferous species and induced mosaic symptoms. Electron microscopy revealed the presence of flexuous virus particles about 750 nm in length. The two isolates were propagated in and purified from mustard plants and were used as immunogens for production of antisera in rabbits. In enzyme-linked immunosorbent assay and sodium dodecyl sulfate-immunodiffusion tests, both antisera reacted strongly with their homologous antigens and with antigens of two Turnip mosaic virus (TuMV) isolates from radish (TuMV-R) and lisianthus (TuMV-L), but not with 21 other different potyviruses tested. In reciprocal tests, antisera against TuMV-R and TuMV-L also reacted strongly with RC4 and YC5 antigens, indicating that these two calla lily isolates are serologically indistinguishable from other known TuMV strains. Cloning and sequence analyses confirmed that both isolates shared 95 to 99% of deduced amino acid sequence identities in the coat protein genes with those of various known TuMV strains. This investigation represents the first record of the natural infection of TuMV in calla lily.

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