Amino acid differences in the N-terminal half of the polyprotein of Chinese turnip mosaic virus isolates affect symptom expression in Nicotiana benthamiana and radish

Infectious clones were generated from 17 new Korean radish isolates of Turnip mosaic virus (TuMV). Phylogenetic analysis indicated that all new isolates, and three previously characterized Korean radish isolates, belong to the basal-BR group (indicating that the pathotype can infect both Brassica and Raphanus spp.). Pairwise analysis revealed genomic nucleotide and polyprotein amino acid identities of >87.9 and >95.7%, respectively. Five clones (HJY1, HJY2, KIH2, BE, and prior isolate R007) had lower sequence identities than other isolates and produced mild symptoms in Nicotiana benthamiana. These isolates formed three distinct sequence classes (HJY1/HJY2/R007, KIH2, and BE), and several differential amino acid residues (in P1, P3, 6K2, and VPg) were present only in mild isolates HJY1, HJY2, and R007. The remaining isolates all induced systemic necrosis in N. benthamiana. Four mild isolates formed a phylogenetic subclade separate from another subclade including all of the necrosis-inducing isolates plus mild isolate KIH2. Symptom severity in radish and Chinese cabbage genotypes was not correlated with pathogenicity in N. benthamiana; indeed, Chinese cabbage cultivar Norang was not infected by any isolate, whereas Chinese cabbage cultivar Chusarang was uniformly susceptible. Four isolates were unable to infect radish cultivar Iljin, but no specific amino acid residues were correlated with avirulence. These results may lead to the identification of new resistance genes against TuMV.

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Nucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance geneTuRB01

Physiological and Molecular Plant Pathology ◽

10.1006/pmpp.1997.0116 ◽

1997 ◽

Vol 51 (3) ◽

pp. 195-208 ◽

Cited By ~ 25

Author(s):

P. Lehmann ◽

K. Petrzik ◽

C. Jenner ◽

A. Greenland ◽

J. Špak ◽

...

Keyword(s):

Amino Acid ◽

Coat Protein ◽

Mosaic Virus ◽

Turnip Mosaic Virus ◽

Coding Region ◽

Protein Coding ◽

Amino Acid Variation ◽

Virus Isolates

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A Turnip Mosaic Virus Determinant of Systemic Necrosis in Nicotiana benthamiana and a Novel Resistance-Breaking Determinant in Chinese Cabbage Identified from Chimeric Infectious Clones

Phytopathology ◽

10.1094/phyto-08-18-0323-r ◽

2019 ◽

Vol 109 (9) ◽

pp. 1638-1647

Author(s):

Ik-Hyun Kim ◽

Hye-Kyoung Ju ◽

Junsu Gong ◽

Jae-Yeong Han ◽

Eun-Young Seo ◽

...

Keyword(s):

Amino Acid ◽

Mosaic Virus ◽

Nicotiana Benthamiana ◽

Potato Virus X ◽

Amino Acid Identity ◽

Turnip Mosaic Virus ◽

Mild Mosaic ◽

Systemic Necrosis ◽

Breeding Lines ◽

Infectious Clones

Infectious clones of Korean turnip mosaic virus (TuMV) isolates KIH1 and HJY1 share 88.1% genomic nucleotides and 96.4% polyprotein amino acid identity, and they induce systemic necrosis or mild mosaic, respectively, in Nicotiana benthamiana. Chimeric constructs between these isolates exchanged the 5′, central, and 3′ domains of KIH1 (K) and HJY1 (H), where the order of the letters indicates the origin of these domains. KIH1 and chimeras KHH and KKH induced systemic necrosis, whereas HJY1 and chimeras HHK, HKK, and HKH induced mild symptoms, indicating the determinant of necrosis to be within the 5′ 3.9 kb of KIH1; amino acid identities of the included P1, Helper component protease, P3, 6K1, and cylindrical inclusion N-terminal domain were 90.06, 98.91, 93.80, 100, and 100%, respectively. Expression of P1 or P3 from a potato virus X vector yielded symptom differences only between P3 of KIH1 and HJY1, implicating a role for P3 in necrosis in N. benthamiana. Chimera KKH infected Brassica rapa var. pekinensis ‘Norang’, which was resistant to both KIH1 and HJY1, indicating that two separate TuMV determinants are required to overcome the resistance. Ability of diverse TuMV isolates, chimeras, and recombinants to overcome resistance in breeding lines may allow identification of novel resistance genes.

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Evaluation of the infectivity of selected turnip mosaic virus isolates towards white cabbage cultivars

Folia Horticulturae ◽

10.2478/fhort-2013-0132 ◽

2009 ◽

Vol 21 (1) ◽

pp. 129-138 ◽

Cited By ~ 4

Author(s):

Karol Gładysz ◽

Ewa Hanus-Fajerska

Keyword(s):

Mosaic Virus ◽

Virus Detection ◽

Turnip Mosaic Virus ◽

Symptom Expression ◽

Mechanical Inoculation ◽

White Cabbage ◽

Resistance Trait ◽

Virus Isolates ◽

Das Elisa ◽

Selection Of

Abstract Experiments were carried out to evaluate the reaction of cabbage cultivars to mechanical inoculation with selected isolates of the turnip mosaic virus (TuMV). Simultaneously we aimed for the assessment of TuMV pathogenicity towards cultivars chosen to be transformed in order to obtain the resistance trait. The TuMV-CAR37A and TuMV-CAR39 isolates from horseradish proved to be infective towards ‘Amager’ and ‘Langedijker’ B. oleracea subsp. capitata f. alba. The course of symptom expression was assessed and the results of virus detection in symptomless leaves, using DAS-ELISA, were documented. Both tested cultivars showed a similar level of susceptibility. TuMV-CAR37A and TuMV-CAR39 can be useful in the selection of cabbage lines with resistance to the turnip mosaic virus.

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Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Viruses ◽

10.3390/v10080430 ◽

2018 ◽

Vol 10 (8) ◽

pp. 430 ◽

Cited By ~ 2

Author(s):

Miroslav Glasa ◽

Katarína Šoltys ◽

Lukáš Predajňa ◽

Nina Sihelská ◽

Slavomíra Nováková ◽

...

Keyword(s):

Mosaic Virus ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Turnip Mosaic Virus ◽

Biological Assays ◽

Genetic Complexity ◽

The World ◽

History Of ◽

Virus Isolates

In recent years, the accumulated molecular data of Turnip mosaic virus (TuMV) isolates from various hosts originating from different parts of the world considerably helped to understand the genetic complexity and evolutionary history of the virus. In this work, four complete TuMV genomes (HC9, PK1, MS04, MS15) were characterised from naturally infected cultivated and wild-growing Papaver spp., hosts from which only very scarce data were available previously. Phylogenetic analyses showed the affiliation of Slovak Papaver isolates to the world-B and basal-B groups. The PK1 isolate showed a novel intra-lineage recombination pattern, further confirming the important role of recombination in the shaping of TuMV genetic diversity. Biological assays indicated that the intensity of symptoms in experimentally inoculated oilseed poppy are correlated to TuMV accumulation level in leaves. This is the first report of TuMV in poppy plants in Slovakia.

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