AFLP and SCAR markers linked to the suppressor gene (Rf) of a dominant genetic male sterility in rapeseed (Brassica napus L.)

Euphytica ◽  
2006 ◽  
Vol 151 (3) ◽  
pp. 401-409 ◽  
Author(s):  
Dengfeng Hong ◽  
Lili Wan ◽  
Pingwu Liu ◽  
Guangsheng Yang ◽  
Qingbiao He
Genome ◽  
2009 ◽  
Vol 52 (9) ◽  
pp. 755-760 ◽  
Author(s):  
Zhenghua Xu ◽  
Yanzhou Xie ◽  
Dengfeng Hong ◽  
Pingwu Liu ◽  
Guangsheng Yang

9012AB, a recessive genic male sterility (RGMS) line derived from spontaneous mutation in Brassica napus , has been playing an important role in rapeseed hybrid production in China. The male sterility of 9012AB is controlled by two recessive genes (ms3 and ms4) interacting with one recessive epistatic suppressor gene (esp). The objective of this study was to develop PCR-based markers tightly linked to the esp gene and construct a high-resolution map surrounding the esp gene. From the survey of 512 AFLP primer combinations, 3 tightly linked AFLP markers were obtained and successfully converted to codominant or dominant SCAR markers. Furthermore, a codominant SSR marker (Ra2G08) associated with the esp gene was identified through genetic map integration. For fine mapping of the esp gene, these PCR-based markers were analyzed in a large BC1 population of 2545 plants. The esp gene was then genetically restricted to a region of 1.03 cM, 0.35 cM from SSR marker Ra2G08 and 0.68 cM from SCAR marker WSC6. The SCAR marker WSC5 co-segregated with the target gene. These results lay a solid foundation for map-based cloning of esp and will facilitate the selection of RGMS lines and their temporary maintainers.


1988 ◽  
Vol 68 (4) ◽  
pp. 1115-1118 ◽  
Author(s):  
LI ◽  
Y. QIAN ◽  
Z. WU ◽  
B. R. STEFANSSON

Genetic male sterility (GMS) discovered in the rape (Brassica napus L.) cultivar, No.23, is conditioned by genes at two loci. The genes were designated as Ms1 and Ms2. Plants homozygous or heterozygous for the dominant Ms1 allele (Ms1Ms1 or Ms1ms1) and homozygous for the recessive ms2 allele (ms2ms2) were sterile while plants homozygous or heterozygous for the dominant Ms2 allele were fertile regardless of the alleles present at the Ms1 locus. The double recessive was also fertile. This indicates dominance epistasis at the Ms2 locus over the Ms1 locus. This GMS system can be used to produce first generation hybrid rape if the required genotypes have been isolaed. Pollination of the homozygous male sterile, Ms1Ms1ms2ms2 with the pollen from the double recessive fertile, ms1ms1ms2 ms2 will produce a generation of sterile plants. These sterile plants (Ms1ms1ms2ms2) when pollinated with pollen from genotypes homozygous for the Ms2 allele (Ms2Ms2), will produce a generation of fertile plants. The various genotypes which can be isolated from this GMS system are being used to develop hybrid rape at the Research Institute of the Shanghai Academy of Sciences.Key words: Rape, genetic male sterility, hybrids, Brassica napus


2007 ◽  
Vol 21 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Xingguo Zhang ◽  
Chaozhi Ma ◽  
Tingdong Fu ◽  
Yuanyuan Li ◽  
Tonghua Wang ◽  
...  

2016 ◽  
Vol 6 ◽  
pp. 9-18
Author(s):  
Aliaa El-Mezawy ◽  
Mohammad Al-Forkan ◽  
Limin Wu ◽  
Randall J. Weselake ◽  
Saleh Shah ◽  
...  

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