Diversity in ploidy levels and nuclear DNA amounts in Korean Miscanthus species

Euphytica ◽  
2013 ◽  
Vol 193 (3) ◽  
pp. 317-326 ◽  
Author(s):  
Youn-Ho Moon ◽  
Young-Lok Cha ◽  
Yong-Hwan Choi ◽  
Young-Mi Yoon ◽  
Bon-Cheol Koo ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Ploidy Levels ◽  
Dna Amounts

Nuclear DNA amount in pure species and hybrid willows (Salix): a flow cytometric investigation

1998 ◽  
Vol 76 (1) ◽  
pp. 157-165 ◽  
Author(s):  
Jérôme Thibault
Keyword(s):  
Flow Cytometry ◽  
Ploidy Level ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Natural Hybridization ◽  
Ploidy Levels ◽  
Key Words Flow Cytometry ◽  
Dna Values ◽  
Dna Amounts

Flow cytometry (FCM) has been used to estimate the nuclear DNA content of 11 Salix species and 5 hybrids. One hundred and sixty nine individuals were studied including 159 individuals from a sequence of 32 communities along a stretch of river in France and 10 individuals from French and English collections for comparison. Isolated nuclei were stained with propidium iodide. FCM was a significantly more practical and rapid technique than that of establishing the karyotype to survey many samples of Salix for variation in ploidy. The 2C DNA amounts for diploid species ranged from 0.76 to 0.98 pg, and tetraploid values ranged from 1.62 to 1.80 pg. The DNA values were consistent with the known ploidy levels. With the exception of a doubtful Salix xquercifolia, ploidy levels and DNA amounts of hybrids were intermediate compared with those of their parents. Intraspecific variation of nuclear DNA values including instrumental variation was low (i.e., 6-11% at the same ploidy level). FCM appeared to be an accurate tool for determination of Salix triploid hybrids. However, it remains limited concerning hybrids from crosses between species of the same ploidy level. Results suggest that natural hybridization might not be frequent in the communities studied, although they have been subject to disturbance. Previous overestimates of hybridization frequency in willows were probably due to misinterpretation of the effects of the environment on Salix spp. morphology; however, the extent and mechanisms of introgression in the genus remain to be further investigated. Key words: flow cytometry, Salix, hybridization, nuclear DNA content, riparian vegetation, disturbance.

scholarly journals Chromosome Number, Ploidy Level, and Nuclear DNA Content in 23 Species of Echeveria (Crassulaceae)

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1950
Author(s):  
Guadalupe Palomino ◽  
Javier Martínez-Ramón ◽  
Verónica Cepeda-Cornejo ◽  
Miriam Ladd-Otero ◽  
Patricia Romero ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Ploidy Level ◽  
Dna Content ◽  
Chromosome Numbers ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Root Tips ◽  
Ploidy Levels ◽  
Dna Amounts

Echeveria is a polyploid genus with a wide diversity of species and morphologies. The number of species registered for Echeveria is approximately 170; many of them are native to Mexico. This genus is of special interest in cytogenetic research because it has a variety of chromosome numbers and ploidy levels. Additionally, there are no studies concerning nuclear DNA content and the extent of endopolyploidy. This work aims to investigate the cytogenetic characteristics of 23 species of Echeveria collected in 9 states of Mexico, analyzing 2n chromosome numbers, ploidy level, nuclear DNA content, and endopolyploidy levels. Chromosome numbers were obtained from root tips. DNA content was obtained from the leaf parenchyma, which was processed according to the two-step protocol with Otto solutions and propidium iodide as fluorochrome, and then analyzed by flow cytometry. From the 23 species of Echeveria analyzed, 16 species lacked previous reports of 2n chromosome numbers. The 2n chromosome numbers found and analyzed in this research for Echeveria species ranged from 24 to 270. The range of 2C nuclear DNA amounts ranged from 1.26 pg in E. catorce to 7.70 pg in E. roseiflora, while the 1C values were 616 Mbp and 753 Mbp, respectively, for the same species. However, differences in the level of endopolyploidy nuclei were found, corresponding to 4 endocycles (8C, 16C, 32C and 64C) in E. olivacea, E. catorce, E. juarezensis and E. perezcalixii. In contrast, E. longiflora presented 3 endocycles (8C, 16C and 32C) and E. roseiflora presented 2 endocycles (8C and 16C). It has been suggested that polyploidization and diploidization processes, together with the presence of endopolyploidy, allowed Echeveria species to adapt and colonize new adverse environments.

scholarly journals Nuclear DNA Amounts in Gymnosperms

1998 ◽  
Vol 82 ◽  
pp. 3-15 ◽  
Author(s):  
B MURRAY
Keyword(s):  
Nuclear Dna ◽  
Dna Amounts

Specific nuclear DNA amounts in toads of the genus Bufo

Chromosoma ◽  
1970 ◽  
Vol 29 (3) ◽  
pp. 365-374 ◽  
Author(s):  
Konrad Bachmann
Keyword(s):  
Nuclear Dna ◽  
Dna Amounts

scholarly journals Nuclear DNA Amounts in Macaronesian Angiosperms

2003 ◽  
Vol 92 (1) ◽  
pp. 153-164 ◽  
Author(s):  
J. SUDA
Keyword(s):  
Nuclear Dna ◽  
Dna Amounts

DNA content in Tradescantia

1985 ◽  
Vol 27 (6) ◽  
pp. 766-775 ◽  
Author(s):  
Arturo Martínez ◽  
Héctor D. Ginzo
Keyword(s):  
North America ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Life Forms ◽  
Chromosome Size ◽  
Polyploid Complex ◽  
Ploidy Levels ◽  
Vegetative Adaptation ◽  
The Relationship

There is a wide variation in the nuclear DNA content and chromosome size between the species belonging to the T. crassifolia and T. virginiana alliances (all the species but one are native to Central and North America). Also the DNA content per genome decreases when the ploidy level increases within the same specific polyploid complex with three ploidy levels (2x, 4x, and 6x). In contrast, no variation was found in the DNA content per genome between different ploidy levels in the T. fluminensis alliance (all the species are native to South America) where they range from 6x to 22x. Since all the species described here are perennials with various life forms, it was possible to analyze the relationship between the DNA content and their vegetative adaptation to the environment. The more specialized species (geophytes and hemicryptophytes) have a higher amount of DNA than the chamaephytes adapted to live in relatively more mesic regions. In the species living in Central and North America there is a positive correlation between the increase in DNA content and the latitude of their native regions.Key words: Tradescantia, DNA content, geographical distribution, life forms, polyploidy.

scholarly journals Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

2011 ◽  
Vol 83 (3) ◽  
pp. 993-1006 ◽  
Author(s):  
Patrícia M.O. Pierre ◽  
Saulo M. Sousa ◽  
Lisete C. Davide ◽  
Marco A. Machado ◽  
Lyderson F. Viccini
Keyword(s):  
Genetic Variation ◽  
Dna Content ◽  
Chromosome Numbers ◽  
Rapd Markers ◽  
Nuclear Dna ◽  
Pollen Viability ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Ploidy Levels ◽  
Lippia Alba

Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of ixoploidy. ur data indicates that sexual reproduction aong those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.

Variation in nuclear DNA amount between wild and cultivated populations of Milium effusum (2n = 28)

Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 1050-1053 ◽  
Author(s):  
Simon T. Bennett ◽  
Michael D. Bennett
Keyword(s):  
Intraspecific Variation ◽  
Nuclear Dna ◽  
Environmental Changes ◽  
Natural Habitat ◽  
Practical Importance ◽  
Plant Conservation ◽  
Plant Responses ◽  
Dna Amount ◽  
Nuclear Dna Amount ◽  
Dna Amounts

Mean 2C DNA amounts varied by 35.6%, ranging from 7.52 to 10.20 pg, between 10 populations of the grass Milium effusum L. Such intraspecific variation occurred despite a constant chromosome number (2n = 28) and no obvious differences in karyotype. Plants originating from botanic garden populations growing in cultivation had significantly (P < 0.001) larger DNA amounts than plants collected from wild populations. Moreover, variation in DNA amount within either the "cultivated" or the "wild" groups was not significant. As the environment in which plants are kept in botanic gardens is clearly different to the natural habitat for M. effusum, it seems likely that the difference in nuclear DNA amount is causally related, perhaps through its nucleotypic effects, to microclimate adaptation. These results suggest that at least some genotypes of M. effusum are fluid and sensitive to environmental change. Such data may have broad practical importance regarding plant responses to various environmental changes such as a nuclear winter and global warming, and implications for plant conservation and reintroduction. Milium effusum is a potentially useful plant material for studying the nature of intraspecific variation in DNA amount.Key words: Milium effusum, nuclear DNA amounts, nucleotype, environmental adaptation.

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