Marker-assisted pyramiding of major blast resistance genes in Swarna-Sub1, an elite rice variety (Oryza sativa L.)

The brown planthopper (BPH: Nilaparvata lugens Stål.) is a major pest of rice, Oryza sativa, in Asia. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper. However, the effectiveness of resistance genes varies spatially and temporally according to BPH virulence. Understanding patterns in BPH virulence against resistance genes is necessary to efficiently and sustainably deploy resistant rice varieties. To survey BPH virulence patterns, seven near-isogenic lines (NILs), each with a single BPH resistance gene (BPH2-NIL, BPH3-NIL, BPH17-NIL, BPH20-NIL, BPH21-NIL, BPH32-NIL and BPH17-ptb-NIL) and fifteen pyramided lines (PYLs) carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, Taichung 65 (T65), and assessed for resistance levels against two BPH populations (Hadano-66 and Koshi-2013 collected in Japan in 1966 and 2013, respectively). Many of the NILs and PYLs were resistant against the Hadano-66 population but were less effective against the Koshi-2013 population. Among PYLs, BPH20+BPH32-PYL and BPH2+BPH3+BPH17-PYL granted relatively high BPH resistance against Koshi-2013. The NILs and PYLs developed in this research will be useful to monitor BPH virulence prior to deploying resistant rice varieties and improve rice’s resistance to BPH in the context of regionally increasing levels of virulence.

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Integrating marker-assisted selection and doubled haploidy for rapid introgression of semi-dwarfing and blast resistance genes into a Basmati rice variety ‘Ranbir Basmati’

Euphytica ◽

10.1007/s10681-019-2473-7 ◽

2019 ◽

Vol 215 (9) ◽

Cited By ~ 1

Author(s):

Prabhudutt Samal ◽

T. D. Pote ◽

S. Gopala Krishnan ◽

Ashok K. Singh ◽

R. K. Salgotra ◽

...

Keyword(s):

Resistance Genes ◽

Marker Assisted Selection ◽

Blast Resistance ◽

Rice Variety ◽

Basmati Rice ◽

Doubled Haploidy ◽

Blast Resistance Genes

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Rice Blast Disease in India: Present Status and Future Challenges

10.5772/intechopen.98847 ◽

2021 ◽

Author(s):

Deepak Chikkaballi Annegowda ◽

Mothukapalli Krishnareddy Prasannakumar ◽

Hirehally Basavarajegowda Mahesh ◽

Chethana Bangera Siddabasappa ◽

Pramesh Devanna ◽

...

Keyword(s):

Resistance Genes ◽

Oryza Sativa L ◽

Blast Resistance ◽

Gene Pyramiding ◽

Climatic Conditions ◽

Blast Disease ◽

Selection Marker ◽

Rice Varieties ◽

Future Challenges ◽

Blast Resistance Genes

Rice (Oryza sativa L.) is the staple food of the majority of Indians, and India is both the major producer and consumer of rice. Rice cultivation in India is confronted with diverse agro-climatic conditions, varying soil types, and several biotic and abiotic constraints. Among major fungal diseases of Rice in India, the blast caused by Magnaporthe oryzae is the most devastating disease, with the neck blast being the most destructive form. Most of the blast epidemic areas in India have been identified with a mixture of races blast fungus resulting in the resistance breakdown in a short period. At present, a more significant number of the rice varieties cultivated in India were bred by conventional breeding methods with blast resistance conferred by a single resistance gene. Therefore, the blast disease in India is predominantly addressed by the use of ecologically toxic fungicides. In line with the rest of the world, the Indian scientific community has proven its role by identifying several blast resistance genes and successfully pyramiding multiple blast resistance genes. Despite the wealth of information on resistance genes and the availability of biotechnology tools, not a great number of rice varieties in India harbor multiple resistance genes. In the recent past, a shift in the management of blast disease in India has been witnessed with a greater focus on basic research and modern breeding tools such as marker-assisted selection, marker-assisted backcross breeding, and gene pyramiding.

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