Genetic variation of a freshwater snail Hydrobioides nassa (Gastropoda: Bithyniidae) in Thailand examined by mitochondrial DNA sequences

Hydrobiologia ◽  
2019 ◽  
Author(s):  
Naruemon Bunchom ◽  
Weerachai Saijuntha ◽  
Warayutt Pilap ◽  
Warong Suksavate ◽  
Kotchaphon Vaisusuk ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Freshwater Snail

scholarly journals Mitochondrial genetic variation is enriched in G-quadruplex regions that stall DNA synthesis in vitro

2020 ◽  
Vol 29 (8) ◽  
pp. 1292-1309 ◽  
Author(s):  
Thomas J Butler ◽  
Katrina N Estep ◽  
Joshua A Sommers ◽  
Robert W Maul ◽  
Ann Zenobia Moore ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Dna Sequences ◽  
Computational Analysis ◽  
Italian Population ◽  
Dna Structures ◽  
G Quadruplex

Abstract As the powerhouses of the eukaryotic cell, mitochondria must maintain their genomes which encode proteins essential for energy production. Mitochondria are characterized by guanine-rich DNA sequences that spontaneously form unusual three-dimensional structures known as G-quadruplexes (G4). G4 structures can be problematic for the essential processes of DNA replication and transcription because they deter normal progression of the enzymatic-driven processes. In this study, we addressed the hypothesis that mitochondrial G4 is a source of mutagenesis leading to base-pair substitutions. Our computational analysis of 2757 individual genomes from two Italian population cohorts (SardiNIA and InCHIANTI) revealed a statistically significant enrichment of mitochondrial mutations within sequences corresponding to stable G4 DNA structures. Guided by the computational analysis results, we designed biochemical reconstitution experiments and demonstrated that DNA synthesis by two known mitochondrial DNA polymerases (Pol γ, PrimPol) in vitro was strongly blocked by representative stable G4 mitochondrial DNA structures, which could be overcome in a specific manner by the ATP-dependent G4-resolving helicase Pif1. However, error-prone DNA synthesis by PrimPol using the G4 template sequence persisted even in the presence of Pif1. Altogether, our results suggest that genetic variation is enriched in G-quadruplex regions that impede mitochondrial DNA replication.

scholarly journals Geographic Patterns of Genetic Variation in a Broadly Distributed Marine Vertebrate: New Insights into Loggerhead Turtle Stock Structure from Expanded Mitochondrial DNA Sequences

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85956 ◽  
Author(s):  
Brian M. Shamblin ◽  
Alan B. Bolten ◽  
F. Alberto Abreu-Grobois ◽  
Karen A. Bjorndal ◽  
Luis Cardona ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Loggerhead Turtle ◽  
Stock Structure ◽  
Geographic Patterns

Genetic variation within African spiny-tailed lizards (Agamidae: Uromastyx) estimated using mitochondrial DNA sequences

2007 ◽  
Vol 28 (1) ◽  
pp. 1-6 ◽  
Author(s):  
José Brito ◽  
Raquel Vaconcelos ◽  
D. James Harris
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Evolutionary History ◽  
Phylogenetic Analyses ◽  
North African ◽  
History Of ◽  
Current Species

AbstractAfrican spiny-tailed lizards (Uromastyx) are large, herbivorous lizards extensively traded locally for food and internationally as pets. Several species have recently been described, although some remain controversial. To determine relationships within North African forms, twenty individuals were analysed for over 1000 bases of mitochondrial DNA sequences. Phylogenetic analyses indicate four deeply divergent lineages that correspond to sampling areas, but not to current species designations. These results indicate that present taxonomy does not reflect the evolutionary history of these species.

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