Genetic variation of Tarbinskiellus portentosus (Lichtenstein 1796) (Orthoptera: Gryllidae) in mainland Southeast Asia examined by mitochondrial DNA sequences

2021 ◽  
Author(s):  
Nakorn Pradit ◽  
Weerachai Saijuntha ◽  
Warayutt Pilap ◽  
Warong Suksavate ◽  
Takeshi Agatsuma ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Southeast Asia ◽  
Dna Sequences ◽  
Mainland Southeast Asia

Genetic variation of a freshwater snail Hydrobioides nassa (Gastropoda: Bithyniidae) in Thailand examined by mitochondrial DNA sequences

Hydrobiologia ◽  
2019 ◽  
Author(s):  
Naruemon Bunchom ◽  
Weerachai Saijuntha ◽  
Warayutt Pilap ◽  
Warong Suksavate ◽  
Kotchaphon Vaisusuk ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Freshwater Snail

scholarly journals Mitochondrial genetic variation is enriched in G-quadruplex regions that stall DNA synthesis in vitro

2020 ◽  
Vol 29 (8) ◽  
pp. 1292-1309 ◽  
Author(s):  
Thomas J Butler ◽  
Katrina N Estep ◽  
Joshua A Sommers ◽  
Robert W Maul ◽  
Ann Zenobia Moore ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Dna Sequences ◽  
Computational Analysis ◽  
Italian Population ◽  
Dna Structures ◽  
G Quadruplex

Abstract As the powerhouses of the eukaryotic cell, mitochondria must maintain their genomes which encode proteins essential for energy production. Mitochondria are characterized by guanine-rich DNA sequences that spontaneously form unusual three-dimensional structures known as G-quadruplexes (G4). G4 structures can be problematic for the essential processes of DNA replication and transcription because they deter normal progression of the enzymatic-driven processes. In this study, we addressed the hypothesis that mitochondrial G4 is a source of mutagenesis leading to base-pair substitutions. Our computational analysis of 2757 individual genomes from two Italian population cohorts (SardiNIA and InCHIANTI) revealed a statistically significant enrichment of mitochondrial mutations within sequences corresponding to stable G4 DNA structures. Guided by the computational analysis results, we designed biochemical reconstitution experiments and demonstrated that DNA synthesis by two known mitochondrial DNA polymerases (Pol γ, PrimPol) in vitro was strongly blocked by representative stable G4 mitochondrial DNA structures, which could be overcome in a specific manner by the ATP-dependent G4-resolving helicase Pif1. However, error-prone DNA synthesis by PrimPol using the G4 template sequence persisted even in the presence of Pif1. Altogether, our results suggest that genetic variation is enriched in G-quadruplex regions that impede mitochondrial DNA replication.

scholarly journals Geographic Patterns of Genetic Variation in a Broadly Distributed Marine Vertebrate: New Insights into Loggerhead Turtle Stock Structure from Expanded Mitochondrial DNA Sequences

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85956 ◽  
Author(s):  
Brian M. Shamblin ◽  
Alan B. Bolten ◽  
F. Alberto Abreu-Grobois ◽  
Karen A. Bjorndal ◽  
Luis Cardona ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Loggerhead Turtle ◽  
Stock Structure ◽  
Geographic Patterns

scholarly journals A new caruncle-bearing fanged frog (Limnonectes, Dicroglossidae) from Laos and Thailand

ZooKeys ◽  
2019 ◽  
Vol 846 ◽  
pp. 133-156
Author(s):  
Somphouthone Phimmachak ◽  
Stephen J. Richards ◽  
Niane Sivongxay ◽  
Sengvilay Seateun ◽  
Yodchaiy Chuaynkern ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Southeast Asia ◽  
Advertisement Call ◽  
Museum Specimens ◽  
Larval Morphology ◽  
Southern Vietnam ◽  
A New Species ◽  
Northeastern Thailand ◽  
Mainland Southeast Asia

A new species of the dicroglossid frog genus Limnonectes is described from recent and historical museum specimens collected in central and southern Laos and northeastern Thailand. Limnonectessavansp. nov. has males that bear a caruncle on top of the head, and most closely resembles L.dabanus from adjacent southern Vietnam and eastern Cambodia. However, the new species is readily distinguished from L.dabanus, and all other caruncle-bearing species of Limnonectes in mainland Southeast Asia, by its adult and larval morphology, mitochondrial DNA, and advertisement call. Its description brings the total number of caruncle-bearing species of Limnonectes to six.

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