Competitive adsorption of bovine serum albumin and lysozyme on characterized calcium phosphates by polyacrylamide gel electrophoresis method

2007 ◽  
Vol 18 (11) ◽  
pp. 2243-2249 ◽  
Author(s):  
X. D. Zhu ◽  
H. S. Fan ◽  
C. Y. Zhao ◽  
J. Lu ◽  
T. Ikoma ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Gel Electrophoresis ◽  
Bovine Serum ◽  
Competitive Adsorption ◽  
Calcium Phosphates ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Electrophoresis Method

scholarly journals Development of Sheep Embryos in vitro in a Medium Supplemented with Different Batches of Serum Albumin

1988 ◽  
Vol 41 (3) ◽  
pp. 371 ◽  
Author(s):  
PA Batt ◽  
BG Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Basal Medium ◽  
Mammalian Embryo ◽  
Molecular Weights ◽  
Mean Molecular Weight

Variability in different lots of commercial serum albumin affects mammalian embryo development in culture. The composition of commercial preparations of ovine, bovine and defatted bovine serum albumin and a fraction of ovine serum containing proteins with a mean molecular weight of 65 kDa (fraction 3) was examined by polyacrylamide gel electrophoresis. All preparations were heavily contaminated with serum proteins other than albumin. Day-6 sheep morulae were cultured for 48 h in a basal bicarbonate-buffered salt solution supplemented with the commercial preparations of ovine, bovine or defatted bovine serum albumin. These three albumin preparations differed in their abilities to support the development of morulae into expanded blastocysts, but these differences disappeared when the basal medium was also supplemented with a component of ovine serum containing substances with molecular weights of less than 10 kDa. In the latter case, the three commercial albumin preparations and fraction 3 of ovine serum all supported full development in about 40-60% of morulae.

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