Heterologous expression of mitochondrial nicotinamide adenine dinucleotide transporter (Ndt1) from Aspergillus fumigatus rescues impaired growth in Δndt1Δndt2 Saccharomyces cerevisiae strain

2017 ◽  
Vol 49 (6) ◽  
pp. 423-435
Author(s):  
Laís de Lourdes de Lima Balico ◽  
Emerson de Souza Santos ◽  
Silveli Suzuki-Hatano ◽  
Lucas Oliveira Sousa ◽  
Ana Elisa Caleiro Seixas Azzolini ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heterologous Expression ◽  
Aspergillus Fumigatus ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine

scholarly journals Identification of NAD+ capped mRNAs in Saccharomyces cerevisiae

2016 ◽  
Vol 114 (3) ◽  
pp. 480-485 ◽  
Author(s):  
Robert W. Walters ◽  
Tyler Matheny ◽  
Laura S. Mizoue ◽  
Bhalchandra S. Rao ◽  
Denise Muhlrad ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nicotinamide Adenine Dinucleotide ◽  
Rna Stability ◽  
Nicotinamide Adenine ◽  
Chemical Modifications ◽  
Mitochondrial Transcripts

RNAs besides tRNA and rRNA contain chemical modifications, including the recently described 5′ nicotinamide-adenine dinucleotide (NAD+) RNA in bacteria. Whether 5′ NAD-RNA exists in eukaryotes remains unknown. We demonstrate that 5′ NAD-RNA is found on subsets of nuclear and mitochondrial encoded mRNAs in Saccharomyces cerevisiae. NAD-mRNA appears to be produced cotranscriptionally because NAD-RNA is also found on pre-mRNAs, and only on mitochondrial transcripts that are not 5′ end processed. These results define an additional 5′ RNA cap structure in eukaryotes and raise the possibility that this 5′ NAD+ cap could modulate RNA stability and translation on specific subclasses of mRNAs.

scholarly journals CYTOCHEMICAL DEMONSTRATION OF GLUTATHIONE REDUCTASE (EC 1.6.4.2) IN SACCHAROMYCES CEREVISIAE

1967 ◽  
Vol 15 (5) ◽  
pp. 273-275 ◽  
Author(s):  
JÜRGEN REISS
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glutathione Reductase ◽  
Nicotinamide Adenine Dinucleotide ◽  
Reduced Glutathione ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Yeast Cells ◽  
Nicotinamide Adenine ◽  
Cytochemical Demonstration ◽  
Ph Level ◽  
Essential Prerequisite

A method for the cytochemical demonstration of glutathione reductase in unfixed yeast cells ( Saccharomyces cerevisiae) is described using reduced glutathione as substrate, nicotinamide adenine dinucleotide phosphate (nicotinamide adenine dinucleotide) as coenzyme and nitro blue tetrazolium as indicator. An essential prerequisite is an exactly controlled pH level (pH 6.5) of the complete incubation medium.

Effect of Niacin on Mitochondria of Allium Cepa Root Cells

1967 ◽  
Vol 25 ◽  
pp. 78-79
Author(s):  
M. Arif Hayat
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Hydrogen Transfer ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Root Tips ◽  
Root Cells ◽  
Transfer Processes ◽  
Standard Procedures ◽  
A Cell ◽  
Critical Interest

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.

Nicotinamide Adenine Dinucleotide and the Metabolism of Ethanol and Acetaldehyde

1967 ◽  
Vol 28 (2) ◽  
pp. 213-224 ◽  
Author(s):  
E. Majchrowicz ◽  
B. L. Bercaw ◽  
W. M. Cole ◽  
D. H. Gregory
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine
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