AbstractOxygen-isotope values of invertebrate cuticle preserved in lake sediments have been used in palaeoenvironmental reconstructions, generally with the assumption that fractionation of oxygen isotopes between cuticle and water ($$\upalpha_{\text{cuticle}-\text{H}_{2}\text{O}}$$
α
cuticle
-
H
2
O
) is independent of temperature. We cultured chironomid larvae in the laboratory with labelled oxygen-isotope water and across a range of closely controlled temperatures from 5 to 25 °C in order to test the hypothesis that fractionation of oxygen isotopes between chironomid head capsules and water ($$\upalpha_{\text{chironomid}-\text{H}_{2}\text{O}}$$
α
chironomid
-
H
2
O
) is independent of temperature. Results indicate that the hypothesis can be rejected, and that $$\upalpha_{\text{chironomid}-\text{H}_{2}\text{O}}$$
α
chironomid
-
H
2
O
decreases with increasing temperature. The scatter in the data suggests that further experiments are needed to verify the relationship. However, these results indicate that temperature-dependence of $$\upalpha_{\text{chironomid}-\text{H}_{2}\text{O}}$$
α
chironomid
-
H
2
O
should be considered when chironomid δ18O is used as a paleoenvironmental proxy, especially in cases where data from chironomids are combined with oxygen-isotope values from other materials for which fractionation is temperature dependent, such as calcite, in order to derive reconstructions of past water temperature.
Correction to: Experimental determination of the temperature dependence of oxygen-isotope fractionation between water and chitinous head capsules of chironomid larvae
