With the increase of new energy power generation, the requirement of smart grid and the popularity of electric vehicles, the research focus on V2G. With Electric vehicles being distributed energy storage or distributed generation, peak regulation in power system is involved in important functions of V2G. In order to achieve peak regulation function, the paper has analyzed the control relationship between the electric vehicles, V2G station and electric vehicle charge\ discharge control center, presented charge and discharge control strategy based on the two levels of electric vehicle charge\discharging control center and V2G station control layer and introduced algorithms and examples to achieve these strategies.