NMR Based Metabonomics Study of DAG Treatment in a C2C12 Mouse Skeletal Muscle Cell Line Myotube Model of Burn-Injury

2011 ◽  
Vol 17 (4) ◽  
pp. 281-299 ◽  
Author(s):  
Miki Watanabe ◽  
Sulaiman Sheriff ◽  
Theresa A. Ramelot ◽  
Nijiati Kadeer ◽  
Junho Cho ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Line ◽  
Muscle Cell ◽  
Burn Injury ◽  
Skeletal Muscle Cell ◽  
Mouse Skeletal Muscle ◽  
Skeletal Muscle Cell Line ◽  
Muscle Cell Line

Activation by curare of acetylcholine receptor channels in a murine skeletal muscle cell line

1989 ◽  
Vol 67 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Catherine E. Morris ◽  
Madeleine R. Montpetit ◽  
Wade J. Sigurdson ◽  
Kuni Iwasa
Keyword(s):  
Skeletal Muscle ◽  
Cell Line ◽  
Acetylcholine Receptor ◽  
Muscle Cell ◽  
Skeletal Muscle Cell ◽  
Single Channels ◽  
Agonist Action ◽  
Murine Skeletal Muscle ◽  
Skeletal Muscle Cell Line ◽  
Muscle Cell Line

Curare action on nicotinic acetylcholine receptors has a number of facets, of which the best known is competitive antagonism. Here we describe the weak agonist action of 10−5 M curare on the murine skeletal muscle cell line, G8. Although curare induces no depolarization in G8 cells, single-channel recordings reveal short-lived curare-induced currents. A feature of these brief events is the multiplicity of conductance levels (of the four levels with conductances of 48, 37, 14, and 6 pS, none had a lifetime greater than 1.5 ms). Most well-resolved events (about 17% of which are to a subconductance) last less than 0.5 ms, with activation occurring predominantly as isolated events rather than in bursts. Agonism is not, however, a high probability action for curare: calculations based on the frequency of events at half-saturating conditions suggest that curare-induced channel openings occur during less than 1% of acetylcholine receptor – curare binding episodes. The outcome is (a) an agonist action too feeble to perturb the membrane voltage and (b) a powerful competitive antagonist action.Key words: curare, acetylcholine receptor, single channels, skeletal muscle.

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