A high-resolution genetic linkage map of soybean based on 357 recombinant inbred lines genotyped with BARCSoySNP6K

A genetic linkage map of lily was constructed using RILs (recombinant inbred lines) population of 180 individuals. This mapping population was developed by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars through single-seed descent (SSD). SRAPs were generated by using restriction enzymes EcoRI in combination with either MseI. The resulting products were separated by electrophoresis on 6% denaturing polyacrylamide gel and visualized by silver staining. The segregation of each marker and linkage analysis was done using the program Mapmaker3.0. With 50 primer pairs, a total of 189 parental polymorphic bands were detected and 78 were used for mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups. The number of markers in the linkage groups varied from 1 to 12. The length of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval distance range from 9.4 cM to 345.4 cM individually. The mean marker interval distance between markers was 27.4 cM. The map developed in the present study was the first sequence-related amplified polymorphism markers map of lily constructed with recombinant inbred lines, it could be used for genetic mapping and molecular marker assisted breeding and quantitative trait locus mapping of Lilium.

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A Genetic Linkage Map for Watermelon Derived from Recombinant Inbred Lines

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.129.2.0237 ◽

2004 ◽

Vol 129 (2) ◽

pp. 237-243 ◽

Cited By ~ 16

Author(s):

Renbing Zhang ◽

Yong Xu ◽

Ke Yi ◽

Haiying Zhang ◽

Ligong Liu ◽

...

Keyword(s):

Linkage Map ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Citrullus Lanatus ◽

Average Distance ◽

Recombinant Inbred Lines ◽

Plant Introduction ◽

Inter Simple Sequence Repeat ◽

Inbred Lines

A genetic linkage map was constructed for watermelon using 117 recombinant inbred lines (RILs) (F2S7) descended from a cross between the high quality inbred line 97103 [Citrullus lanatus var. lanatus (Thunb.) Matsum. & Nakai] and the Fusarium wilt (races 0, 1, and 2) resistant U.S. Plant Introduction (PI) 296341 (C. lanatus var. citroides). The linkage map contains 87 randomly amplified polymorphic DNA (RAPD) markers, 13 inter simple sequence repeat (ISSR) markers, and four sequenced characterized amplified region (SCAR) markers. The map consists of 15 linkage groups. Among them are a large linkage group of 31 markers covering a mapping distance of 277.5 cM, six groups each with 4 to 12 markers covering a mapping distance of 51.7 to 172.2 cM, and eight small groups each with 2-5 markers covering a mapping distance of 7.9 to 46.4 cM. The map covers a total distance of 1027.5 cM with an average distance of 11.7 cM between two markers. The map is useful for the further development of quantitative trait loci (QTLs) affecting fruit qualities and for identification of genes conferring resistance to Fusarium wilt (races 0, 1 and 2). The present map can be used for further construction of a reference linkage map for watermelon based on an immortalized mapping population with progenies homozygous for most gene loci.

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High-density genetic linkage map based on arbitrary and microsatellite markers using inter-specific recombinant inbred lines in eggplant (Solanum melongena L.)

Journal of Plant Biochemistry and Biotechnology ◽

10.1007/s13562-020-00549-w ◽

2020 ◽

Vol 29 (3) ◽

pp. 427-438

Author(s):

Pallavi Mishra ◽

Shailesh K. Tiwari ◽

Sarvesh P. Kashyap ◽

Kavindra N. Tiwari ◽

Major Singh ◽

...

Keyword(s):

Microsatellite Markers ◽

Linkage Map ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Recombinant Inbred Lines ◽

Solanum Melongena ◽

Inbred Lines ◽

High Density

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A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines

Theoretical and Applied Genetics ◽

10.1007/s001220050869 ◽

1998 ◽

Vol 97 (1-2) ◽

pp. 83-89 ◽

Cited By ~ 83

Author(s):

I. Eujayl ◽

M. Baum ◽

W. Powell ◽

W. Erskine ◽

E. Pehu

Keyword(s):

Linkage Map ◽

Recombinant Inbred ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Aflp Markers

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An improved genetic linkage map for cowpea (Vigna unguiculata L.) Combining AFLP, RFLP, RAPD, biochemical markers, and biological resistance traits

Genome ◽

10.1139/g01-102 ◽

2002 ◽

Vol 45 (1) ◽

pp. 175-188 ◽

Cited By ~ 83

Author(s):

J T Ouédraogo ◽

B S Gowda ◽

M Jean ◽

T J Close ◽

J D Ehlers ◽

...

Keyword(s):

Molecular Markers ◽

Linkage Map ◽

Genetic Linkage ◽

Genetic Linkage Map ◽

Fragment Length Polymorphism ◽

Recombinant Inbred Lines ◽

Inbred Lines ◽

Resistance Gene Analogs ◽

Biological Resistance ◽

Resistance Traits

An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population of 94 recombinant inbred lines (RILs) derived from the cross between 'IT84S-2049' and '524B'. A set of 242 molecular markers, mostly amplified fragment length polymorphism (AFLP), linked to 17 biological resistance traits, resistance genes, and resistance gene analogs (RGAs) were scored for segregation within the parental and recombinant inbred lines. These data were used in conjunction with the 181 random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), AFLP, and biochemical markers previously mapped to construct an integrated linkage map for cowpea. The new genetic map of cowpea consists of 11 linkage groups (LGs) spanning a total of 2670 cM, with an average distance of 6.43 cM between markers. Astonishingly, a large, contiguous portion of LG1 that had been undetected in previous mapping work was discovered. This region, spanning about 580 cM, is composed entirely of AFLP markers (54 in total). In addition to the construction of a new map, molecular markers associated with various biological resistance and (or) tolerance traits, resistance genes, and RGAs were also placed on the map, including markers for resistance to Striga gesnerioides races 1 and 3, CPMV, CPSMV, B1CMV, SBMV, Fusarium wilt, and root-knot nematodes. These markers will be useful for the development of tools for marker-assisted selection in cowpea breeding, as well as for subsequent map-based cloning of the various resistance genes.Key words: Cowpea, AFLP, RAPD, resistance gene analogs, genetic linkage map.

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