Identification and mapping of two adult plant leaf rust resistance genes in durum wheat

Leaf rust, caused by Puccinia triticina Eriks., is the most common disease of wheat (Triticum aestivum L.) in the United States and worldwide. The objective of this study was to characterize seedling and adult plant leaf rust resistance in hard red spring wheat cultivars grown in Minnesota, North Dakota, and South Dakota, and postulate the identity of the seedling leaf rust resistance genes in the cultivars. Twenty-six cultivars, near-isogenic lines of Thatcher wheat that differ for single leaf rust resistance genes, and three wheat cultivars with known leaf rust resistance genes, were tested with 11 different isolates of leaf rust collected from the United States and Canada. The leaf rust infection types produced on seedling plants of the cultivars in greenhouse tests were compared with the infection types produced by the same isolates on the Thatcher near-isogenic lines to postulate which seedling leaf rust resistance genes were present. Seedling leaf rust resistance genes Lr1, Lr2a, Lr10, Lr16, Lr21, and Lr24 were postulated to be present in spring wheat cultivars. Seedling genes Lr3, Lr14a, and Lr23 likely were present in some cultivars but could not be clearly identified in this study. Most of the cultivars had some level of adult plant leaf rust resistance, most likely due to Lr34. Cultivars that had seedling resistance genes Lr1, Lr2a, Lr10, or Lr16 had poor to intermediate levels of leaf rust resistance in field plots. Cultivars with combinations of seedling resistance genes Lr16 and Lr24 with additional adult plant resistance were highly resistant to leaf rust.

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Physiologic Specialization of Puccinia triticina in Canada in 1998

Plant Disease ◽

10.1094/pdis.2001.85.2.155 ◽

2001 ◽

Vol 85 (2) ◽

pp. 155-158 ◽

Cited By ~ 35

Author(s):

J. A. Kolmer

Keyword(s):

Leaf Rust ◽

Bread Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Wheat Cultivars ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

In 1998, leaf rust of wheat (Triticum aestivum), caused by Puccinia triticina, was widespread throughout the prairies of western Canada. Warm summer temperatures with frequent dew periods favored spread of the disease in wheat fields in Manitoba and Saskatchewan. The Canada Prairie Spring wheat cultivars (AC Vista, AC Foremost, AC Crystal) were susceptible to leaf rust, while the bread wheat cultivars with leaf rust resistance genes Lr16 and Lr13 or Lr34 (AC Majestic, AC Domain, AC Barrie) had high to moderate levels of leaf rust infections. Bread wheat cultivars AC Cora, AC Minto, Pasqua, and McKenzie had trace to low levels of leaf rust infection. Thirty-four virulence phenotypes of P. triticina were identified on 16 Thatcher lines, which are near-isogenic for leaf rust resistance genes. Phenotypes with virulence to Lr16 increased to 25% of isolates in Manitoba and Saskatchewan in 1998. Forty-three isolates were also tested for virulence to plants with the adult plant resistance genes Lr12, Lr13, Lr34, and Lr13,34. Most isolates had virulence to Lr12 and Lr13. All isolates had lower infection type on adult plants with Lr34 compared with Thatcher.

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Inheritance of Leaf Rust Resistance in Wheat Cultivars Grandin and CDC Teal

Plant Disease ◽

10.1094/pdis.1997.81.5.505 ◽

1997 ◽

Vol 81 (5) ◽

pp. 505-508 ◽

Cited By ~ 17

Author(s):

J. Q. Liu ◽

J. A. Kolmer

Keyword(s):

Leaf Rust ◽

Spring Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Wheat Cultivars ◽

Hard Red Spring Wheat ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

The hard red spring wheat cultivars Grandin and CDC Teal were genetically examined to determine the number and identity of the leaf rust resistance genes present in both wheats. The two cultivars were crossed with the leaf rust susceptible cultivar Thatcher, and the F1 plants were backcrossed to Thatcher. Fifty-four and 80 BC1F1 plants derived respectively from Grandin and CDC Teal were selfed to produce BC1F2 families. The BC1F2 families were tested as seedlings with isolates of Puccinia recondita f. sp. tritici that differed for virulence to specific leaf rust resistance genes. The BC1F2 families were also tested in the adult-plant stage in field rust nursery tests. Segregation of BC1F2 families in the seedling tests indicated that Grandin had resistance genes Lr2a, Lr3, and Lr10, and was heterogeneous for Lr16. CDC Teal was shown to have the seedling leaf rust gene Lr1. In field rust nursery tests, both Grandin and CDC Teal were shown to have adult-plant resistance genes Lr13 and Lr34. Additional leaf rust resistance genes that condition effective field resistance should be incorporated into hard red spring wheat cultivars to diversify the leaf rust resistance in this wheat class.

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Quantitative Trait Loci Conferring Leaf Rust Resistance in Hexaploid Wheat

Phytopathology ◽

10.1094/phyto-06-18-0208-rvw ◽

2018 ◽

Vol 108 (12) ◽

pp. 1344-1354 ◽

Cited By ~ 16

Author(s):

Gerarda Beatriz Pinto da Silva ◽

Camila Martini Zanella ◽

José Antônio Martinelli ◽

Márcia Soares Chaves ◽

Colin W. Hiebert ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Resistance Genes ◽

Quantitative Trait ◽

Hexaploid Wheat ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

Leaf rust, caused by the fungal pathogen Puccinia triticina, is a major threat to wheat production in many wheat-growing regions of the world. The introduction of leaf rust resistance genes into elite wheat germplasm is the preferred method of disease control, being environmentally friendly and crucial to sustained wheat production. Consequently, there is considerable value in identifying and characterizing new sources of leaf rust resistance. While many major, qualitative leaf rust resistance genes have been identified in wheat, a growing number of valuable sources of quantitative resistance have been reported. Here we review the progress made in the genetic identification of quantitative trait loci (QTL) for leaf rust resistance detected primarily in field analyses, i.e., adult plant resistance. Over the past 50 years, leaf rust resistance loci have been assigned to genomic locations through chromosome analyses and genetic mapping in biparental mapping populations, studies that represent 79 different wheat leaf rust resistance donor lines. In addition, seven association mapping studies have identified adult plant and seedling leaf rust resistance marker trait associations in over 4,000 wheat genotypes. Adult plant leaf rust resistance QTL have been found on all 21 chromosomes of hexaploid wheat, with the B genome carrying the greatest number of QTL. The group 2 chromosomes are also particularly rich in leaf rust resistance QTL. The A genome has the lowest number of QTL for leaf rust resistance. [Formula: see text] Copyright © 2018 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

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Genetics of Leaf Rust Resistance in Spring Wheat Cultivars Alsen and Norm

Phytopathology ◽

10.1094/phyto-95-0773 ◽

2005 ◽

Vol 95 (7) ◽

pp. 773-778 ◽

Cited By ~ 35

Author(s):

L. M. Oelke ◽

J. A. Kolmer

Keyword(s):

Leaf Rust ◽

Spring Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Wheat Cultivar ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Plant Genes ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

Alsen is a recently released spring wheat cultivar that has been widely grown in the United States because it has resistance to Fusarium head blight and leaf rust caused by Puccinia triticina. Norm is a high yielding wheat cultivar that has been very resistant to leaf rust since it was released. Alsen and Norm were genetically examined to determine the number and identity of the leaf rust resistance genes present in both wheats. The two cultivars were crossed with leaf rust susceptible cv. Thatcher and F1 plants were backcrossed to Thatcher. Eighty one and seventy three BCF1 of Thatcher times; Alsen and Thatcher × Norm respectively, were selfed to obtain BCF2 families. The BCF2 families were tested as seedlings with different isolates of P. triticina that differed for virulence to specific leaf rust resistance genes. The BCF2 families that lacked seedling resistance were also tested as adult plants in greenhouse tests and in a field rust nursery plot. Segregation of BCF2 families indicated that Alsen had seedling genes Lr2a, Lr10, and Lr23 and adult plant genes Lr13 and Lr34. Norm was determined to have seedling genes Lr1, Lr10, Lr16, and Lr23 and adult plant genes Lr13 and Lr34. The characterization of Lr23 in the segregating populations was complicated by the presence of a suppressor gene in Thatcher and the high temperature sensitivity of resistance expression for this gene. The effective leaf rust resistance in Alsen is due to the interaction of Lr13 and Lr23, with Lr34; and the effective leaf rust resistance in Norm is due to the interaction of Lr13, Lr16, and Lr23, with Lr34.

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Genetic Analysis of Leaf Rust Resistance in Six Durum Wheat Genotypes

Phytopathology ◽

10.1094/phyto-03-14-0065-r ◽

2014 ◽

Vol 104 (12) ◽

pp. 1322-1328 ◽

Cited By ~ 8

Author(s):

Alexander Loladze ◽

Dhouha Kthiri ◽

Curtis Pozniak ◽

Karim Ammar

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Durable Resistance ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Seedling Resistance ◽

Allelism Tests

Leaf rust, caused by Puccinia triticina, is one of the main fungal diseases limiting durum wheat production. This study aimed to characterize previously undescribed genes for leaf rust resistance in durum wheat. Six different resistant durum genotypes were crossed to two susceptible International Maize and Wheat Improvement Center (CIMMYT) lines and the resulting F1, F2, and F3 progenies were evaluated for leaf rust reactions in the field and under greenhouse conditions. In addition, allelism tests were conducted. The results of the study indicated that most genotypes carried single effective dominant or recessive seedling resistance genes; the only exception to this was genotype Gaza, which carried one adult plant and one seedling resistance gene. In addition, it was concluded that the resistance genes identified in the current study were neither allelic to LrCamayo or Lr61, nor were they related to Lr3 or Lr14a, the genes that already are either ineffective or are considered to be vulnerable for breeding purposes. A complicated allelic or linkage relationship between the identified genes is discussed. The results of the study will be useful for breeding for durable resistance by creating polygenic complexes.

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Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Crop Science ◽

10.1002/csc2.20057 ◽

2020 ◽

Vol 60 (1) ◽

pp. 282-302 ◽

Cited By ~ 1

Author(s):

Matthew J. Martin ◽

Oswaldo Chicaiza ◽

Juan C. Caffarel ◽

Ahmad H. Sallam ◽

Arnis Druka ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Introgression Lines ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

Open Life Sciences ◽

10.1515/biol-2019-0036 ◽

2019 ◽

Vol 14 (1) ◽

pp. 327-334 ◽

Cited By ~ 2

Author(s):

Agnieszka Tomkowiak ◽

Roksana Skowrońska ◽

Alicja Buda ◽

Danuta Kurasiak-Popowska ◽

Jerzy Nawracała ◽

...

Keyword(s):

Multiplex Pcr ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Field Resistance ◽

Wheat Cultivars ◽

Pcr Conditions ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

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