Conditioned medium enhances the fusion capability of rat bone marrow mesenchymal stem cells and cardiomyocytes

2014 ◽  
Vol 41 (5) ◽  
pp. 3099-3112 ◽  
Author(s):  
Kanwal Haneef ◽  
Nadia Naeem ◽  
Irfan Khan ◽  
Hana’a Iqbal ◽  
Nurul Kabir ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

scholarly journals Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation

2018 ◽  
Vol 22 (1) ◽  
pp. 20
Author(s):  
Ekayanti Mulyawati Kaiin ◽  
Wahono Esthi Prasetyaningtyas ◽  
Kusdiantoro Mohamad ◽  
Ita Djuwita ◽  
Tuty Laswardi Yusuf ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Positive Reaction ◽  
Leydig Cells ◽  
Bioactive Materials ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.

scholarly journals Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation

2018 ◽  
Vol 22 (1) ◽  
pp. 20
Author(s):  
Ekayanti Mulyawati Kaiin ◽  
Wahono Esthi Prasetyaningtyas ◽  
Kusdiantoro Mohamad ◽  
Ita Djuwita ◽  
Tuty Laswardi Yusuf ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Positive Reaction ◽  
Leydig Cells ◽  
Bioactive Materials ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.

Dosage and Passage Dependent Neuroprotective Effects of Exosomes Derived from Rat Bone Marrow Mesenchymal Stem Cells: An In Vitro Analysis

2018 ◽  
Vol 18 ◽  
Author(s):  
Chaitra Venugopal ◽  
Christopher Shamir ◽  
Sivapriya Senthilkumar ◽  
Janitri Venkatachala Babu ◽  
Peedikayil Kurien Sonu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuroprotective Effects ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Rat Bone

Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration

2004 ◽  
Vol 362 (3) ◽  
pp. 200-203 ◽  
Author(s):  
Mel Tohill ◽  
Cristina Mantovani ◽  
Mikael Wiberg ◽  
Giorgio Terenghi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Nerve Regeneration ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone
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