The genetic characterization of Clarias gariepinus was conducted in this
study. Thirty (30) C. gariepinus specimens were collected, fifteen (15) each
from the wild and farms in northeastern Nigeria for their genetic
relatedness and diversity using the RAPD markers. DNA extraction from the
blood sample was performed using the Gene Jet Genomic DNA Purification Kit.
Five primers were used in employing PCR and amplified 402 RAPD bands from
the four strains of Clarias gariepinus. Three hundred and six (76.12%) bands
were polymorphic while 86 (21.39%) were monomorphic. The percentage of
polymorphism obtained from farmed and wild populations ranged from 58
(47.3%) to 69 (75.9%), respectively. The polymorphic bands per loci within
the populations ranged from 67.9% to 82.0%. The mean numbers of inbreeding
coefficient (FIS) were 0.083 and 0.053 in the farmed and wild populations.
Gene diversity values within farmed and wild populations (Ht) were 0.4522
and 0.4018. The mean genetic differentiation (FST) ranged between (0.203) in
the farmed and 0.129 in the wild populations. The analysis of molecular
variance revealed that there was 96% of genetic variance within the
population and 4% among the population. The genetic identity and distance of
four populations were 0.9490 and 0.1038, respectively. The phylogenetic
measure has shown that the four strains were divided into two clusters at
approximately 0.089 similarity levels. The result indicated a significant
level of genetic variation and minimal dendrogram separation in Clarias
gariepinus in northeastern Nigeria. Conclusively, this information will be a
useful tool for the genetic and breeding program of Clarias gariepinus.
Detection and Genetic Characterization of Enteroviruses Circulating among Wild Populations of Chimpanzees in Cameroon: Relationship with Human and Simian Enteroviruses
