Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton

2020 ◽  
Vol 47 (2) ◽  
pp. 1275-1282
Author(s):  
Juanjuan Feng ◽  
Haiyong Zhu ◽  
Meng Zhang ◽  
Xuexian Zhang ◽  
Liping Guo ◽  
...  
Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1383-1394
Author(s):  
Roger P Wise ◽  
Carren L Dill ◽  
Patrick S Schnable

Abstract Dominant alleles of the rf1 and rf2 nuclear-encoded fertility restorer genes are necessary for restoration of pollen fertility in T-cytoplasm maize. To further characterize fertility restoration mediated by the Rf1 allele, 123,500 gametes derived from plants carrying the Mutator transposable element family were screened for rf1-mutant alleles (rf1-m) Four heritable rf1-m alleles were recovered from these populations. Three rf1-m alleles were derived from the progenitor allele Rf1-IAl53 and one was derived from Rf1-Ky21. Cosegregation analysis revealed 5.5- and 2.4kb Mu1-hybridizing EcoRI restriction fragments in all of the male-sterile and none of the male-fertile plants in families segregating for rf1-m3207 and rf1-m3310, respectively. Mitochondrial RNA gel blot analyses indicated that all four rf1-m alleles in male-sterile plants cosegregated with the altered steady-state accumulation of 1.6 and O.6-kb T-urf13 transcripts, demonstrating that these transcripts are Rf1 dependent. Plants carrying a leaky mutant, rf1-m7323, revealed variable levels of Rf1-associated, T-urf13 transcripts and the degree of pollen fertility. The ability to obtain rf1-m derivatives from Rf1 indicates that Rf1 alleles produce a functional gene product necessary for the accumulation of specific T-urf13 transcripts in T-cytoplasm maize.


2017 ◽  
Vol 136 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Thomas Miedaner ◽  
Cathérine Pauline Herter ◽  
Heike Goßlau ◽  
Peer Wilde ◽  
Bernd Hackauf

2009 ◽  
Vol 35 (6) ◽  
pp. 367-370 ◽  
Author(s):  
I. N. Anisimova ◽  
V. A. Gavrilova ◽  
V. T. Rozhkova ◽  
G. I. Timofeeva ◽  
M. A. Tikhonova

1985 ◽  
Vol 27 (5) ◽  
pp. 487-490 ◽  
Author(s):  
Surinder S. Banga ◽  
K. S. Labana

Male sterile plants of Indian mustard (Brassica juncea (L.) Coss.) were observed in the F2 generation of the cross RLM-198 × EJ-33. The genetic analysis revealed that male sterility occurred when the cytoplasm of RLM-198 interacted with recessive nuclear genes of EJ-33. The genetic constitution of RLM-198 was postulated to be (S) RF RF, EJ-33 as (F) rf rf, and the male sterile plants as (S) rf rf. Varieties of Indian mustard from India mostly contained dominant fertility restorer genes, while European varieties had a greater frequency of the recessive maintainer genes. None of these varieties, however, was capable of complete maintenance of male sterility. Heterosis for yield up to 56% over the national check was observed in field trials. The use of this cytoplasmic male sterile plant in hybrid mustard production will not be economical, until a complete maintainer for male sterility is identified.Key words: Brassica juncea, Indian mustard, male sterility, hybrids.


Euphytica ◽  
2006 ◽  
Vol 153 (1-2) ◽  
pp. 35-42 ◽  
Author(s):  
Majid Sattari ◽  
Arumugam Kathiresan ◽  
Glenn B. Gregorio ◽  
Jose E. Hernandez ◽  
Tamerlane M. Nas ◽  
...  

Author(s):  
Anirban Nath ◽  
Disharee Nath ◽  
Chand Kumar Santra ◽  
Tapash Dasgupta

The current study extensively evaluates 51 genotypes for their fertility restoration potential using test crosses with five WA (wild abortive) Cytoplasmic Male Sterile lines namely IR58025A, IR6897A, IR79156A, IR80559A and APMS6A. Also the genotypes were screened using SSR markers RM6100 and RM10313, tightly linked with the fertility restorer genes Rf4 and Rf3 respectively. The two way approach helped in identifying potential restorers for five WA-CMS lines and also detected the presence of dominant Rf genes in their genetic background. The R-lines identified can be safely presumed to be strong restorers for consecutive A-lines they were crossed with. The study also identified a potential maintainer CN1039-9 for the A-line IR58025A. The maintainer line identified can be later exploited for developing new CMS lines.


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