Synergistic effect of copper and amino acid mixtures on the production of extracellular matrix proteins in skin fibroblasts

2021 ◽  
Author(s):  
Ki Wung Chung ◽  
Seh Hyon Song ◽  
Man-seok Kim
Keyword(s):  
Extracellular Matrix ◽  
Amino Acid ◽  
Synergistic Effect ◽  
Extracellular Matrix Proteins ◽  
Skin Fibroblasts ◽  
Matrix Proteins ◽  
Amino Acid Mixtures ◽  
Effect Of Copper

Comparative proteomic analysis of extracellular matrix proteins secreted by two types of skin fibroblasts

PROTEOMICS ◽  
2006 ◽  
Vol 6 (21) ◽  
pp. 5868-5879 ◽  
Author(s):  
Delphine Pflieger ◽  
Sandrine Chabane ◽  
Olivier Gaillard ◽  
Bruno Alain Bernard ◽  
Patrick Ducoroy ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Proteomic Analysis ◽  
Extracellular Matrix Proteins ◽  
Skin Fibroblasts ◽  
Matrix Proteins ◽  
Comparative Proteomic Analysis

scholarly journals Diversity of ace, a Gene Encoding a Microbial Surface Component Recognizing Adhesive Matrix Molecules, from Different Strains of Enterococcus faecalis and Evidence for Production of Ace during Human Infections

2000 ◽  
Vol 68 (9) ◽  
pp. 5210-5217 ◽  
Author(s):  
Sreedhar R. Nallapareddy ◽  
Kavindra V. Singh ◽  
Ruay-Wang Duh ◽  
George M. Weinstock ◽  
Barbara E. Murray
Keyword(s):  
Amino Acids ◽  
Extracellular Matrix ◽  
Amino Acid ◽  
Enterococcus Faecalis ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Rabbit Serum ◽  
Collagen Types ◽  
Collagen Binding ◽  
Different Strains

ABSTRACT Our previous work reported that most Enterococcus faecalis strains adhered to the extracellular matrix proteins collagen types I and IV and laminin after growth at 46°C, but not 37°C, and we subsequently identified an E. faecalissequence, ace, that encodes a bacterial adhesin similar to the collagen binding protein Cna of Staphylococcus aureus. In this study, we examined the diversity of E. faecalis-specific ace gene sequences among different isolates obtained from various geographic regions as well as from various clinical sources. A comparison of nucleotide and deduced amino acid sequences of Ace from nine E. faecalis strains identified a highly conserved N-terminal A domain, followed by a variable B domain which contains two to five repeats of 47 amino acids in tandem array, preceded by a 20-amino-acid partial repeat. Using 17 other strains collected worldwide, the 5′ region of acethat encodes the A domain was sequenced, and these sequences showed ≥97.5% identity. Among the previously reported five amino acids critical for collagen binding by Cna of S. aureus, four were found to be identical in Ace from all strains tested. Polyclonal immune rabbit serum prepared against recombinant Ace A derived fromE. faecalis strain OG1RF detected Ace in mutanolysin extracts of seven of nine E. faecalis strains after growth at 46°C; Ace was detected in four different molecular sizes that correspond to the variation in the B repeat region. To determine if there was any evidence to indicate that Ace might be produced under physiological conditions, we quantitatively assayed sera collected from patients with enterococcal infections for the presence of anti-Ace A antibodies. Ninety percent of sera (19 of 21) from patients withE. faecalis endocarditis showed reactivity with titers from 1:32 to >1:1,024; the only 2 sera which lacked antibodies to Ace A had considerably lower titers of antibodies to other E. faecalis antigens as well. Human-derived, anti-Ace A immunoglobulins G purified from an E. faecalis endocarditis patient serum inhibited adherence of 46°C-grown E. faecalis OG1RF to collagen types I and IV and laminin. In conclusion, these results show that ace is highly conserved among isolates of E. faecalis, with at least four variants related to the differences in the B domain, is expressed by different strains during infection in humans, and human-derived antibodies can block adherence to these extracellular matrix proteins.

Extracellular matrix proteins control the growth of cerebellar medulloblastoma cells

2004 ◽  
Vol 216 (03) ◽  
Author(s):  
U Schüller ◽  
W Hartmann ◽  
A Koch ◽  
K Schilling ◽  
OD Wiestler ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Cerebellar Medulloblastoma

ECMSRC: A Sparse Learning Approach for the Prediction of Extracellular Matrix Proteins

2017 ◽  
Vol 12 (4) ◽  
Author(s):  
Imran Naseem ◽  
Shujaat Khan ◽  
Roberto Togneri ◽  
Mohammed Bennamoun
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Sparse Learning ◽  
Learning Approach
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