Phenotyping and validation of molecular markers associated with rust resistance genes in wheat cultivars in Egypt

2021 ◽  
Author(s):  
Adel A. Elshafei ◽  
Waled M. El-Orabey ◽  
Fathallah B. Fathallah ◽  
Ramadan M. Esmail ◽  
Mohamed A. Abou-Zeid
Keyword(s):  
Molecular Markers ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Wheat Cultivars ◽  
Rust Resistance Genes

scholarly journals Identification of stem rust resistance genes in wheat cultivars in China using molecular markers

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4882 ◽  
Author(s):  
Xiaofeng Xu ◽  
Depeng Yuan ◽  
Dandan Li ◽  
Yue Gao ◽  
Ziyuan Wang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Genetic Material ◽  
Stem Rust Resistance ◽  
Wheat Cultivars ◽  
Wheat Stem Rust ◽  
Stem Rust Resistance Genes ◽  
Rust Resistance Genes

Wheat stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn. (Pgt), is a major disease that has been effectively controlled using resistance genes. The appearance and spread of Pgt races such as Ug99, TKTTF, and TTTTF, which are virulent to most stem rust-resistant genes currently deployed in wheat breeding programs, renewed the interest in breeding cultivars resistant to wheat stem rust. It is therefore important to investigate the levels of resistance or vulnerability of wheat cultivars to Pgt races. Resistance to Pgt races 21C3CTHQM, 34MKGQM, and 34C3RTGQM was evaluated in 136 Chinese wheat cultivars at the seedling stage. A total of 124 cultivars (91.2%) were resistant to the three races. Resistance genes Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38 were analyzed using molecular markers closely linked to them, and 63 of the 136 wheat cultivars carried at least one of these genes: 21, 25, and 28 wheat cultivars likely carried Sr2, Sr31, and Sr38, respectively. Cultivars “Kehan 3” and “Jimai 22” likely carried Sr25. None of the cultivars carried Sr24 or Sr26. These cultivars with known stem rust resistance genes provide valuable genetic material for breeding resistant wheat cultivars.

scholarly journals Molecular Markers Identification of Leaf Rust Resistant Genes Lr19, Lr21, Lr24, Lr47 and Lr51 in Selected Egyptian Wheat Cultivars

2015 ◽  
Vol 4 (2) ◽  
pp. 55-62
Author(s):  
Ashraf M.M. Abdelbacki ◽  
Reda I. Omara ◽  
Nor E.K. Soliman ◽  
Mohammed A. Najeeb
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Dynamic Change ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Rust Disease ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused by Puccinia triticina is a common and widespread disease of bread wheat (Triticum aestivum L.), in Egypt. Host resistance is the most economical, effective and ecologically sustainable method for controlling the disease. Molecular markers help to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. The objective of this study was to evaluate and detect leaf rust resistance genes in Egyptian wheat cultivars. Ten out of fifteen cultivars were resistance to leaf rust disease in four locations i.e., Dakahlia, Kafr el-Sheikh, Beheira and Sharqia during seasons 2011/2012 and 2012/2013. As for, using specific SSR primers proved that Lr19 was present in five cultivars i.e., Sakha-95, Gemmeiza-9, Gemmeiza-10, Misr-1 and Misr-2. Lr21. Lr24, Lr47, and Lr51 were detected in all tested cultivars. These genes should be taken into consideration in wheat breeding programs for successful rust resistance. Furthermore these materials can be used as a parent for plant breeders to add new effective resistance genes to their breeding materials because of the dynamic change of leaf rust races which can breakdown the resistance.

scholarly journals Identification of Leaf Rust Resistance Genes in Selected Egyptian Wheat Cultivars by Molecular Markers

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
I. A. Imbaby ◽  
M. A. Mahmoud ◽  
M. E. M. Hassan ◽  
A. R. M. Abd-El-Aziz
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Rust Disease ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused byPuccinia triticinaEriks., is a common and widespread disease of wheat (Triticum aestivumL.) in Egypt. Host resistance is the most economical, effective, and ecologically sustainable method of controlling the disease. Molecular markers help to determine leaf rust resistance genes (Lrgenes). The objective of this study was to identifyLrgenes in fifteen wheat cultivars from Egypt. Ten genes,Lr13,Lr19,Lr24,Lr26,Lr34,Lr35 Lr36,Lr37,Lr39, andLr46, were detected in fifteen wheat cultivars using various molecular markers. The most frequently occurring genes in fifteen Egyptian wheat cultivars wereLr13,Lr24,Lr34, andLr36identified in all the cultivars used, followed byLr26andLr35(93%),Lr39(66%),Lr37(53%), andLr46(26.6%) of the cultivars, and finallyLr19was present in 33.3% of cultivars. It is concluded that there was a good variation inLrgenes carried by wheat cultivars commercially grown in Egypt. Therefore, strategies for deploying resistance genes to prolong effective disease resistance are suggested to control wheat leaf rust disease.

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