Monitoring of the Cytoskeleton-Dependent Intracellular Trafficking of Fluorescent Iron Oxide Nanoparticles by Nanoparticle Pulse-Chase Experiments in C6 Glioma Cells

2018 ◽  
Vol 43 (11) ◽  
pp. 2055-2071 ◽  
Author(s):  
Wiebke Willmann ◽  
Ralf Dringen
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Intracellular Trafficking ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Oxide Nanoparticles

scholarly journals Evaluation of Toxicity and Neural Uptake In Vitro and In Vivo of Superparamagnetic Iron Oxide Nanoparticles

2018 ◽  
Vol 19 (9) ◽  
pp. 2613 ◽  
Author(s):  
Muhammad Khalid ◽  
Muhammad Asad ◽  
Petra Henrich-Noack ◽  
Maxim Sokolov ◽  
Werner Hintz ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Glioma Cells ◽  
Superparamagnetic Iron Oxide ◽  
C6 Glioma ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
C6 Glioma Cells ◽  
Oxide Nanoparticles

Superparamagnetic iron oxide nanoparticles (SPIO-NPs) have great potential to be used in different pharmaceutical applications, due to their unique and versatile physical and chemical properties. The aim of this study was to quantify in vitro cytotoxicity of dextran 70,000-coated SPIO-NPs labelled/unlabelled with rhodamine 123, in C6 glioma cells and primary hippocampal neural cells. In addition, we analyzed the in vitro and in vivo cellular uptake of labelled SPIO-NPs. The nanoparticles, with average size of 10–50 nm and polydispersity index of 0.37, were synthesized using Massart’s co-precipitation method. The concentration-dependent cytotoxicity was quantified by using tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Intracellular localization of SPIO-NPs was detected by confocal laser microscopy. In vivo confocal neuroimaging (ICON) was performed on male Wistar rats after intravitreal injection followed by ex vivo retina whole mount analysis. When used for in vitro testing concentrations in the range of diagnostic and therapeutic dosages, SPIO-NPs proved to be non-cytotoxic on C6 glioma cells for up to 24 h incubation time. The hippocampal cell culture also did not show impaired viability at low doses after 24 h incubation. Our results indicate that our dextran-coated SPIO-NPs have the potential for in vivo drug delivery applications.

Superparamagnetic iron oxide nanoparticles enhance glioma radiosensitivity via inducing cell cycle arrest and apoptosis

2020 ◽  
Author(s):  
Jinning Mao ◽  
Meng Jiang ◽  
Xingliang Dai ◽  
Guodong Liu ◽  
Zhixiang Zhuang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Glioma Cells ◽  
Superparamagnetic Iron Oxide ◽  
Spherical Shape ◽  
Treated Group ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
X Ray

Abstract Aim: Superparamagnetic iron oxide nanoparticles (SPIONs) is a widely used biomedical material for imaging and targeting drug delivery. We synthesized SPIONs and tested their effects on the radiosensitization of glioma.Methods: Acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (Fe3O4 NPs) were synthesized via a one-step hydrothermal approach and the surface was chemically modified with acetic anhydride to generate surface charge-neutralized NPs. NPs were characterized by TEM and ICP-AES. Radiosensitivity of U87MG glioma cells was evaluated by MTT assay. Cell cycle and apoptosis in glioma cells were examined by flow cytometry. Results: APTS-coated Fe3O4 NPs had a spherical or quasi-spherical shape with average size of 10.5±1.1 nm. NPs had excellent biocompatibility and intracellular uptake of NPs reached the peak 24 hours after treatment. U87 cell viability decreased significantly after treatment with both X-ray and NPs compared to X-ray treatment alone. Compared to X-ray treatment alone, the percentage of cells in G2/M phase (31.83%) significantly increased in APTS-coated Fe3O4 NPs plus X-ray treated group (P<0.05). In addition, the percentage of apoptotic cells was significant higher in APTS-coated Fe3O4 NPs plus X-ray treated group than in X-ray treatment alone group (P<0.05). Conclusion: APTS-coated Fe3O4 NPs achieved excellent biocompatibility and increased radiosensitivity for glioma cells.

Assembly of polyethylenimine-functionalized iron oxide nanoparticles as agents for DNA transfection with magnetofection technique

2014 ◽  
Vol 2 (45) ◽  
pp. 7936-7944 ◽  
Author(s):  
Lei Zhang ◽  
Yecheng Li ◽  
Jimmy C. Yu ◽  
Ying Ying Chen ◽  
King Ming Chan
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Intracellular Trafficking ◽  
Oxide Nanoparticles ◽  
Dna Transfection ◽  
Dna Carriers

In this work two PEI-functionalized magnetic DNA carriers were prepared for DNA transfection, and the intracellular trafficking of magnetofectins was studied.

In Vitro Cytotoxicity of Transparent Yellow Iron Oxide Nanoparticles on Human Glioma Cells

2010 ◽  
Vol 10 (12) ◽  
pp. 8550-8555 ◽  
Author(s):  
Yun Wang ◽  
Mo-Tao Zhu ◽  
Bing Wang ◽  
Meng Wang ◽  
Hua-Jian Wang ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Glioma Cells ◽  
Oxide Nanoparticles ◽  
Human Glioma ◽  
Human Glioma Cells

scholarly journals Lactoferrin conjugated iron oxide nanoparticles for targeting brain glioma cells in magnetic particle imaging

Nanoscale ◽  
2015 ◽  
Vol 7 (40) ◽  
pp. 16890-16898 ◽  
Author(s):  
Asahi Tomitaka ◽  
Hamed Arami ◽  
Sonu Gandhi ◽  
Kannan M. Krishnan
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Particle ◽  
Glioma Cells ◽  
Oxide Nanoparticles ◽  
Brain Glioma ◽  
Magnetic Particle Imaging ◽  
Particle Imaging

Monodisperse lactoferrin conjugated iron oxide nanoparticles were synthesized and evaluated forin vitroglioma imaging using Magnetic Particle Imaging (MPI).

scholarly journals Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma

2021 ◽  
Vol 22 (9) ◽  
pp. 4556
Author(s):  
Didem Ag Seleci ◽  
Viktor Maurer ◽  
Firat Baris Barlas ◽  
Julian Cedric Porsiel ◽  
Bilal Temel ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Glioma Cells ◽  
Oxide Nanoparticles ◽  
Enhancement Effect ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Nanoscale Systems ◽  
Magnetic Iron Oxide

The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEGNIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.

scholarly journals Iron-Doping of Copper Oxide Nanoparticles Lowers Their Toxic Potential on C6 Glioma Cells

2020 ◽  
Vol 45 (4) ◽  
pp. 809-824 ◽  
Author(s):  
Arundhati Joshi ◽  
Hendrik Naatz ◽  
Kathrin Faber ◽  
Suman Pokhrel ◽  
Ralf Dringen
Keyword(s):  
Copper Oxide ◽  
Glioma Cells ◽  
Copper Oxide Nanoparticles ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Oxide Nanoparticles ◽  
Iron Doping ◽  
Toxic Potential
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