Abstract
Background: Both NH4+ and NO3– are capable of greatly influencing plants’ growth and biomass. However, the belowground responses of subtropical trees to either NH4+ or NO3– deposition remain poorly understood. Here, we discuss how these two forms of N deposition can affect root development, and experimentally analyzed how they could impact nitrogen and phosphorus absorption in two types (broadleaved with a fibrous root system vs. conifer with a tap root system) of subtropical tree species. Results: In a greenhouse in southern China, 1-year-old S. superba and P. massoniana seedlings grown on P-limited and P-normal soil were treated with NaNO3 and NH4Cl solutions of 0, 80, and 200 kg N ha–1 year–1, corresponding to the control, N80, and N200 groups, respectively. Root phenotype characteristics and metabolism ability were measured after 8 months of growth. The results showed that the root morphology and physiology variables differed significantly between the two species under different N and P treatments. Although S. superba had a larger quantity of roots than P. massoniana, both its root growth rate and root absorption were respectively lower and weaker. N addition differentially affected root growth and activity as follows: (1) NO3–-N80 and NH4+-N80 increased root growth and activity of the two species, but NH4+-N80 led to thicker roots in S. superba; (2) NO3–-N200 and NH4+-N200 had inhibitory effects on the roots of P. massoniana, for which NH4+-N200 led to thinner and longer roots and even the death of some roots; and (3) NH4+-N could promote metabolic activity in thicker roots (> 1.5 mm) and the NO3–-N was found to stimulate activity in thinner roots (0.5–1.5 mm) in the fibrous root system having a larger quantity of roots, namely S. superba. By contrast, NO3–-N and NH4+-N had an opposite influence upon functioning in the tap root system with a slender root, namely P. massoniana. Conclusion: We conclude P. massoniana has a much higher root absorption efficiency; however, nitrogen deposition is more beneficial to the root growth of S. superba.
Adaptation Mechanism of Roots to Low and High Nitrogen Revealed by Proteomic Analysis
