Improved frequency of transformation in rice and maize by treatment of immature embryos with centrifugation and heat prior to infection with Agrobacterium tumefaciens

2006 ◽  
Vol 87 (3) ◽  
pp. 233-243 ◽  
Author(s):  
Yukoh Hiei ◽  
Yuji Ishida ◽  
Keisuke Kasaoka ◽  
Toshihiko Komari
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Immature Embryos

scholarly journals LaCl3 Treatment Improves Agrobacterium-mediated Immature Embryo Genetic Transformation Frequency of Maize

2021 ◽  
Author(s):  
Shengnan Liu ◽  
Yunlu Shi ◽  
Fang Liu ◽  
Yan Guo ◽  
minhui Lu
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Molecular Breeding ◽  
Channel Blocker ◽  
Immature Embryo ◽  
Transformation Frequency ◽  
Immature Embryos ◽  
Infection Frequency ◽  
Novel Method ◽  
Regenerated Plantlets

Abstract Agrobacterium-mediated genetic transformation of immature embryos is important for gene-function studies and molecular breeding of maize. However, the relatively low genetic transformation frequency remains a bottleneck for applicability of this method, especially on commercial scale. We report that pretreatment of immature embryos with LaCl3 (a Ca2+ channel blocker) improves the infection frequency of Agrobacterium tumefaciens, increases the proportion of positive calluses, yields more positive regenerated plantlets, and increases the transformation frequency from 8.40% to 17.60% for maize. This optimization is a novel method for improving the frequency of plant genetic transformations mediated by Agrobacterium tumefaciens.

Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens

2011 ◽  
Vol 10 (3) ◽  
pp. 317-326 ◽  
Author(s):  
Li-li TAO ◽  
Gui-xiang YIN ◽  
Li-pu DU ◽  
Zheng-yuan SHI ◽  
Mao-yun SHE ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Regeneration ◽  
Immature Embryos

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba
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