scholarly journals High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of In vitro Derived ‘Nadia’ Ginger Microrhizome

2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Dikash Singh THINGBAIJAM ◽  
Devi Sunitibala HUIDROM
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Regeneration System ◽  
Transverse Thin Cell Layer

scholarly journals High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of In vitro Derived ‘Nadia’ Ginger Microrhizome

2014 ◽  
Vol 6 (1) ◽  
pp. 85-91
Author(s):  
Dikash Singh THINGBAIJAM ◽  
Devi Sunitibala HUIDROM
Keyword(s):  
High Frequency ◽  
Somatic Embryos ◽  
Cell Layer ◽  
Sucrose Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Thin Cell Layer ◽  
Regeneration System ◽  
Transverse Thin Cell Layer ◽  
2,4 Dichlorophenoxyacetic Acid

An efficient and reproducible procedure is outlined for rapid in vitro multiplication of Zingiber officinale var. ‘Nadia’ through high frequency shoot proliferation from transverse thin cell layer (tTCL) sections of in vitro derived microrhizome. In vitro derived microrhizome of size 500 μm in thickness was used as initial explants for induction of somatic embryos. Among the different phytohormones tested, tTCL explants shows maximum calli proliferation in medium containing 2 mg/L 2,4-Dichlorophenoxyacetic acid (88.30±0.11%). Reduced concentration of 2,4 Dichlorophenoxyacetic acid was supplemented with different cytokinins for regeneration of callus. Among the different medium tested, optimum redifferentiation of somatic embryos were observed in medium containing 0.2 mg/L 2,4 Dichlorophenoxyacetic acid and 6.0 mg/L BAP (141.08±0.25). Clump of regenerated plantlets were further subculture and transfer into microrhizome inducing medium containing high sucrose concentration (8%). Plantlets with well developed microrhizome were successfully acclimatized and eventually transferred to the field. The application of studying embryo section for regeneration of plants might be useful alternative to ginger improvement programme. Histological analysis showed formation of somatic embryos and regenerated adventitious shoot.

Micropropagation of Rosa damascena Mill. through transverse thin cell layer culture

2012 ◽  
Vol 2 (4) ◽  
pp. 184-189
Author(s):  
Kavita Kshirsagar ◽  
V. J. Braganza
Keyword(s):  
Shoot Regeneration ◽  
Large Scale ◽  
Cell Layer ◽  
In Vitro Regeneration ◽  
Rosa Damascena ◽  
Ms Medium ◽  
Thin Cell Layer ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture

A basic factor underlying the success of large‐scale micropropagation and genetic transformation of any plant species is regeneration. In order to regenerate propagules of Rosa damascena Mill. on a large scale, an efficient and improved in vitro propagation system has been established using transverse thin cell layer culture (tTCL). By optimizing the position of the tissue and applying an improved selection procedure, in vitro shoots were elongated in 8 weeks of culture. Modified Murashige and Skoog (1962)(MS) medium fortified with 4.0 mg l‐1 6‐benzylaminopurine (BAP) and 0.4mg l‐1 anaphthalene acetic acid (NAA) gave optimal shoot regeneration. The explant was inoculated on this medium in the upright position and exhibited a high frequency of shoot regeneration (~96.66%), and it also gave the highest number of shoots (22.33/explant). The horizontally placed explant on an average 7.66 shoots/explant. Our experiments indicate that explant orientation strongly influences the organogenesis response. The frequency of shoot initiation and the number of multiple shoots produced from each explant were significantly dependent on the plant source, concentration of plant growth regulators and the orientation of the explants and contributed significantly to in vitro regeneration. Rooting of well developed shoots was achieved on hormone free ¼ strength MS medium with 4% sucrose.

scholarly journals In vitro micropropagation of Anthurium andraeanum through thin cell layer culture

2018 ◽  
Vol 15 (2) ◽  
pp. 319-326
Author(s):  
Trần Thị Ngọc Lan ◽  
Trần Thị Hoàn Anh
Keyword(s):  
Callus Culture ◽  
Cell Layer ◽  
Callus Formation ◽  
Casein Hydrolysate ◽  
Thin Cell Layer ◽  
Regeneration System ◽  
Regeneration Rate ◽  
Embryogenic Cells ◽  
Anthurium Andreanum

Anthurium is a kind of major cut flower species and economically important genera in the family Araceae. A regeneration system of Anthurium andreanum from callus culture is studied along with improving propagation process. Optimal medium for callus formation from the longitudinal thin cell layer leaf culture was ½ MS (Murashige, Skoog, 1962) supplemented with 30 g/l sucrose, 1 g/l casein hydrolysate (CH), 8 g/l agar, 1.5 mg/1 BA and 0.2 mg/1 2,4-D (the induction callus rate of 77.33%). Calli multiplied with 21.45-fold fresh mass increase when they were subcultured once 60 days on the ½ MS supplemented with 1 g/l CH, 30 g/l sucrose, 1,5 mg/l BA and 8 g/l agar. The highest ratio of shoot induced from callus culture was also on this medium (18.54 shoots per explants). The medium ½ MS supplemented with 20 g/l sucrose, 1 g/l AC, 8 g/l agar and without plant growth regulators is the most suitable one for root formation of these multiple-shoots. The highest survival rate is observed with substrate mixture of fern moss and rice husk ash in the ratio of 1:1 (gave the best regeneration rate of 100% in the ex vitro culture) and no morphological variations were observed in these plantlets after about thirty days,acclimatized in the greenhouse. Histological observation showed that these calli contained embryogenic cells with their fast growth.It could be an alternative tool for propagation and preservation of Anthurium andreanum, a valuable cultivar.

scholarly journals High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

2015 ◽  
Vol 3 (2) ◽  
pp. 202-209 ◽  
Author(s):  
R. Shashi Kumar ◽  
V. Krishna ◽  
. Venkatesh
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Synergetic Effect ◽  
Multiple Shoot ◽  
Coconut Water ◽  
Morphological Variations ◽  
Musa Paradisiaca ◽  
Shoot Initiation

High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 %) was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l), TDZ (0.1 to 1.2 mg/l) and coconut water (0.1 to 0.9 ml/l) induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg), number of hands in a bunch, number of fingers in a hand, length of the finger (cm), girth of the finger (cm) and girth of the pseudostem (cm) exhibited by in vitro plants were higher than the in vivo plants.Int J Appl Sci Biotechnol, Vol 3(2): 202-209 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12536 

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