In vitro manipulations in St. John’s wort (Hypericum perforatum L.) for incessant and scale up micropropagation using adventitious roots in liquid medium and assessment of clonal fidelity using RAPD analysis

2008 ◽  
Vol 96 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Manoj K. Goel ◽  
A. K. Kukreja ◽  
N. S. Bisht
Keyword(s):  
Liquid Medium ◽  
Adventitious Roots ◽  
Hypericum Perforatum ◽  
Rapd Analysis ◽  
Scale Up ◽  
Clonal Fidelity ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L

scholarly journals Development of a Micropropagation Protocol for St. John's Wort (Hypericum perforatum L.)

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 978-980 ◽  
Author(s):  
Joe-Ann McCoy ◽  
N.D. Camper
Keyword(s):  
Depressive Disorders ◽  
Hypericum Perforatum ◽  
Leaf Disc ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
St John’S Wort ◽  
St John's Wort ◽  
Explant Source ◽  
Hypericum Perforatum L

Hypericum perforatum L. (St. John's Wort) has an extensive history as an important medicinal herb used for the treatment of neurological and depressive disorders (Linde et al., 1996). The objective of this study was to establish an in vitro tissue culture protocol for St. John's Wort. Nodal segments, axillary buds, and leaf disc explants produced multiple shoots and callus on Murashige and Skoog minimal organics medium supplemented with combinations of indoleacetic acid (IAA; 0.57, 2.85, 5.71 μm) and benzylaminopurine (BA; 2.22, 4.44, 8.88 μm). Shoot production occurred on all combinations of IAA/BA tested and was significantly less in treatments without hormones. Callus production was higher on treatments containing 2.85 μm IAA + 4.44 μm BA, or 5.71 μm IAA + 8.88 μm BA. Shoots transferred to hormone-free medium at 8 weeks formed roots by 12 weeks. A micropropagation protocol was established for St. John's Wort using mature plants as the explant source.

scholarly journals In Vitro Conservation of Medicinal Plant St. John’s Wort (Hypericum perforatum L.) Through Dilution of Basic Medium

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Sitti Fatimah Syahid
Keyword(s):  
Hypericum Perforatum ◽  
In Vitro Conservation ◽  
Basic Medium ◽  
Minimal Growth ◽  
St John’S Wort ◽  
St John's Wort ◽  
Necrotic Symptom ◽  
Hypericum Perforatum L ◽  
Culture Growth

In vitro techniques could be applied for plant conservation through minimal growth. Growth reduction is generally attained by modifying the culture medium and/or the environmental condition. Conservation of St. John’s wort (Hypericum perforatum L.) in vitro through dilution of basic medium was conducted at the Tissue Culture Laboratory of Indonesian Spice and Medicinal Crops Research Institute (ISMCRI) in 2018. Sterile shoots were cultured into Murashige and Skoog (MS) medium by reducing macronutrients. The treatments used were full-MS + 0.1 mg L-1 N6 - benzyladenine (BA) as control; ¾ MS + 0.1 mg L-1 BA; ½ MS + 0.1 mgL-1 BA and ¼ MS + 0.1 mg L-1 BA. Observation of the culture growth was conducted three months after the treatments. The experiment was arranged in Completely Randomized Design with ten replications. The result showed that the use of a dilution of basic medium affected the growth of St. John’s wort. The use of ¼ MS + 0.1 mg L-1 BA composition could suppress culture growth (number of shoots, shoots length and number of leaves), without showing necrotic symptom until three months of conservation. This treatment could be used as an alternative to minimize the culture of St. John’s wort for in vitro conservation.

Sign in / Sign up

Export Citation Format

Share Document