A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos

2009 ◽  
Vol 99 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Maurecilne Lemes da Silva ◽  
Daniela Lopes Paim Pinto ◽  
Miguel Pedro Guerra ◽  
Eny Iochevet Segal Floh ◽  
Cláudio Horst Bruckner ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Passion Fruit ◽  
Zygotic Embryos ◽  
Regeneration System ◽  
Fruit Species ◽  
Wild Passion Fruit

Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences

PROTOPLASMA ◽  
2011 ◽  
Vol 249 (3) ◽  
pp. 747-758 ◽  
Author(s):  
Diego Ismael Rocha ◽  
Lorena Melo Vieira ◽  
Francisco André Ossamu Tanaka ◽  
Luzimar Campos da Silva ◽  
Wagner Campos Otoni
Keyword(s):  
Somatic Embryogenesis ◽  
Passion Fruit ◽  
Fruit Species ◽  
Wild Passion Fruit

Repetitive somatic embryogenesis from wild passion fruit (Passiflora cincinnata Mast.) anthers

2021 ◽  
Author(s):  
Maurecilne Lemes da Silva ◽  
Daniela Lopes Paim Pinto ◽  
José Marcello Salabert de Campos ◽  
Ilio Fealho de Carvalho ◽  
Diego Ismael Rocha ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Passion Fruit ◽  
Repetitive Somatic Embryogenesis ◽  
Wild Passion Fruit

Somatic embryogenesis from zygotic embryos and shoot-tips of the Chilean tree Gomortega keule

2011 ◽  
Vol 109 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Diego Muñoz-Concha ◽  
Sean Mayes ◽  
Gracia Ribas ◽  
Michael R. Davey
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Tips ◽  
Zygotic Embryos ◽  
Gomortega Keule

scholarly journals Induction of Somatic Embryogenesis in Vulnerable Medicinal Plant Hedychium spicatum Buch-Ham ex Smith

2014 ◽  
Vol 23 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Dinesh Giri ◽  
Sushma Tamta
Keyword(s):  
Somatic Embryogenesis ◽  
Medicinal Plant ◽  
Somatic Embryos ◽  
Synthetic Seeds ◽  
Ex Situ ◽  
Secondary Embryogenesis ◽  
Zygotic Embryos ◽  
High Concentration ◽  
Cotyledon Explants ◽  
Short Period

This protocol has been developed for somatic embryogenesis in Hedychium spicatum. Simultaneously, a method has also been developed for the production of synthetic seeds by using somatic embryos. Direct somatic embryos were developed on cotyledon explants of zygotic embryos on MS supplemented with high concentration of NAA (20.0 µM). Induction of secondary embryogenesis was best in 2,4-D supplemented medium fortified with activated charcoal. Germination of somatic embryos was enhanced by using GA3. Besides this, round and semi-hard beads of somatic embryos (synthetic seeds) could be produced by using 2% Na-alginate and 100 mM calcium chloride and more than 30% germination of synthetic seeds was achieved in MS. Well acclimated plants produced via somatic embryogenesis and/or synthetic seeds were transferred to field where more than 60% survived. This simple study enabled us to obtain a number of plantlets throughout the year each cycle requiring a short period of time. Besides propagation, this study provided an ex situ method for conservation of this vulnerable Himalayan species.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17506Plant Tissue Cult. & Biotech. 23(2): 147-155, 2013  (December)

scholarly journals Can Ethylene Inhibitors Enhance the Success of Olive Somatic Embryogenesis?

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 168
Author(s):  
Muhammad Ajmal Bashir ◽  
Cristian Silvestri ◽  
Amelia Salimonti ◽  
Eddo Rugini ◽  
Valerio Cristofori ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Salicylic Acid ◽  
Silver Nitrate ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Cobalt Chloride ◽  
Zygotic Embryos ◽  
Ethylene Inhibitors ◽  
Embryogenic Calli ◽  
Stimulatory Action

An efficient in vitro morphogenesis, specifically through somatic embryogenesis, is considered to be a crucial step for the application of modern biotechnological tools for genetic improvement in olive (Olea europaea L.). The effects of different ethylene inhibitors, i.e., cobalt chloride (CoCl2), salicylic acid (SA), and silver nitrate (AgNO3), were reported in the cyclic somatic embryogenesis of olive. Embryogenic callus derived from the olive immature zygotic embryos of the cultivar Leccino, was transferred to the expression ECO medium, supplemented with the ethylene inhibitors at 20 and 40 µM concentrations. Among these, the maximum number of somatic embryos (18.6) was obtained in media containing silver nitrate (40 µM), followed by cobalt chloride (12.2 somatic embryos @ 40 µM) and salicylic acid (40 µM), which produced 8.5 somatic embryos. These compounds interfered on callus traits: white friable embryogenic calli were formed in a medium supplemented with 40 µM cobalt chloride and salicylic acid; in addition, a yellow-compact embryogenic callus appeared at 20 µM of all the tested ethylene inhibitors. The resulting stimulatory action of silver nitrate among all the tested ethylene inhibitors on somatic embryogenesis, clearly demonstrates that our approach can efficiently contribute to the improvement of the current SE protocols for olive.

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