An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii

2010 ◽  
Vol 105 (2) ◽  
pp. 271-283 ◽  
Author(s):  
S. Paul ◽  
A. Dam ◽  
A. Bhattacharyya ◽  
T. K. Bandyopadhyay
2015 ◽  
Vol 25 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Asmaa M Abdel Salam ◽  
Kamal Chowdhury ◽  
Ahmed A El Bakry

The effect of sugar types, age of culture, and concentrations of 2,4?D and sucrose on somatic embryogenesis was investigated in Cymbopogon schoenanthus subsp. proximus. Six different sugar types: sucrose, fructose, maltose, glucose, galactose and lactose at 3% concentration were used for each of three 2,4?D concentration treatments (1.0, 2.0, 4.0 mg/l). ANOVA showed a significant difference at the 1% level for culture age, sugar types and interactions between 2,4?D and sugar types when mean embryo numbers of individual sugars across the four culture ages are compared. Maltose gave highest mean value, followed by sucrose and the lowest mean number was recorded with galactose. Cultures induced by galactose although low in embryo induction gave the highest mean number of shoots across the different media and culture age. Effect of different concentrations of sucrose and BA on production of number of immature somatic embryos, mature germinating somatic embryos and shoots was examined by culturing 2.5 month?old seed?derived callus onto MS medium containing 4 different sucrose concentrations (1.0, 2.0, 3.0, 4.0 %) in combination with different BA concentrations (0.0, 0.025, 0.05, 0.1 mg/l). ANOVA showed significant differences at 1% level for culture age, and interactions between culture age and sucrose concentrations. Mean number of shoots on 1% sucrose for 7 months old culture was 9.4 significantly higher than number produced on all of sucrose concentrations. The efficient regeneration system will be useful for the future production of high yielding genotypes and for the conservation of the species germplasm.Plant Tissue Cult. & Biotech. 25(1): 51-62, 2015 (June)


2012 ◽  
Vol 112 (3) ◽  
pp. 387-393 ◽  
Author(s):  
Chellappan Soundar Raju ◽  
Krishnan Kathiravan ◽  
Abubakker Aslam ◽  
Appakan Shajahan

Biologia ◽  
2010 ◽  
Vol 65 (3) ◽  
Author(s):  
Laura Solís-Ramos ◽  
Sara Nahuath-Dzib ◽  
Antonio Andrade-Torres ◽  
Felipe Barredo-Pool ◽  
Tomas González-Estrada ◽  
...  

AbstractCapsicum chinense is recalcitrant in in vitro morphogenesis. No efficient, reproducible somatic embryogenesis regeneration system exists for this species, impeding regeneration from transformed cells. An indirect somatic embryogenesis protocol is developed using mature C. chinense zygotic embryo segments (ZES). The ZES cultured in semi-solid Murashige-Skoog (MS) medium supplemented with 8.9 μM naphthaleneacetic acid, 11.4 μM indoleacetic acid and 8.9 μM 6-benzylaminopurine, developed an embryogenic callus and 8% of the calli developed somatic embryos. Torpedo-stage somatic embryos were detached from the callus and subcultured in semi-solid MS medium without growth regulators, producing a 75% conversion rate to plantlets with well-formed root tissue. Histological analysis showed the developed structures to have no vascular connection with the callus and to be bipolar, confirming that this protocol induced formation of viable somatic embryos from mature C. chinense ZES. All acclimated plantlets survived under greenhouse conditions. This protocol will facilitate regeneration of genetically transformed plants using either biolistics or Agrobacterium tumefaciens approach.


2011 ◽  
Vol 106 (2) ◽  
pp. 337-344 ◽  
Author(s):  
Sergio Cerezo ◽  
José A. Mercado ◽  
Fernando Pliego-Alfaro

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.


2017 ◽  
Vol 226 ◽  
pp. 184-190 ◽  
Author(s):  
Seyyed Javad Mousavizadeh ◽  
Kambiz Mashayekhi ◽  
Mohammad Reza Hassandokht

2018 ◽  
Vol 93 (4) ◽  
pp. 1285-1295 ◽  
Author(s):  
Afshan Naaz ◽  
Sheikh Altaf Hussain ◽  
Ruphi Naz ◽  
Mohammad Anis ◽  
Abdulrahman A. Alatar

2020 ◽  
Vol 25 (5) ◽  
pp. 1886-1891
Author(s):  
MARIA-MIHAELA ANTOFIE ◽  
◽  
MONICA ELENA MITOI

Author(s):  
Tammy Estabrooks ◽  
Zhongmin Dong

Somatic embryogenesis is the process by which somatic cells are induced into an embryogenic state, followed by differentiation into embryos. Somatic embryogenesis, in addition to being a method of propagation, can serve as an experimental tool for research into plant embryo development. This is a review of the current literature on in vitro plant somatic embryogenesis and the molecular advances made to identify genes expressed during the various stages of this process. Some factors hindering the elucidation of the molecular mechanisms underlying somatic embryogenesis are discussed.L’embryogenèse somatique est le processus par lequel les cellules somatiques passent à l’état embryogène et se différencient en embryons. En plus de constituer une méthode de propagation, elle peut servir d’outil expérimental de recherche pour développer des embryons de plantes. Le présent document est une revue de la documentation sur l’embryogenèse somatique végétale in vitro et sur les progrès réalisés à l’échelle moléculaire pour identifier les gènes exprimés au cours des divers stades du processus. On examine aussi certains facteurs qui rendent difficile l’élucidation des mécanismes moléculaires de l’embryogenèse somatique.


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